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Comparative intraosseal growth of human prostate cancer cell lines LNCaP and PC-3 in the nude mouse.
Anticancer Res. 1997 Nov-Dec; 17(6D):4253-8.AR

Abstract

BACKGROUND

More than 75% of patients with advanced prostate carcinoma have skeletal involvement, which is the principal metastatic site and the major complication of this disease. The goal of this work was to compare the osseous metastasis of androgen-sensitive and insensitive prostate cancers in the nude mouse.

MATERIALS AND METHODS

Androgen-sensitive LNCaP or -insensitive PC-3 human prostate carcinoma cells were injected directly into the femur medullas of male nude Beige mice, the animals were then sacrificed at successive time intervals to study the gross and microscopic characteristics of the established tumors.

RESULTS

LNCaP and PC-3 both colonized in the bone marrow within a week, then gradually expanded to the entire bone medulla followed by osseous infiltration to produce obvious symptoms in the affected extremities. Based on the morphology, both osteoblastic and osteolytic changes occurred during the course of tumor progression. In addition, PC-3 tumors eventually broke through the bone cortex, invaded the surrounding tissues, and metastasized to the regional lymph nodes. In contrast, LNCaP remained localized within the bone, and appeared to eventually regress and die after displacing the normal bone marrow cells. Immunohistochemically, LNCaP tumors were consistently positive for prostate-specific antigen in bone metastasis, while PC-3 tumors were negative. Tumor cell nuclei of both PC-3 and LNCaP hybridized to a human repeated sequence DNA probe indicating that the proliferating malignant cells were of human origin.

CONCLUSIONS

These cancer cell lines produced a high incidence of growth in the bone that differed in histogenesis. The relative malignancy of these cell lines was demonstrated in this model.

Authors+Show Affiliations

Department of Urology, State University of New York Health Science Center, Syracuse 13210, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9494517

Citation

Soos, G, et al. "Comparative Intraosseal Growth of Human Prostate Cancer Cell Lines LNCaP and PC-3 in the Nude Mouse." Anticancer Research, vol. 17, no. 6D, 1997, pp. 4253-8.
Soos G, Jones RF, Haas GP, et al. Comparative intraosseal growth of human prostate cancer cell lines LNCaP and PC-3 in the nude mouse. Anticancer Res. 1997;17(6D):4253-8.
Soos, G., Jones, R. F., Haas, G. P., & Wang, C. Y. (1997). Comparative intraosseal growth of human prostate cancer cell lines LNCaP and PC-3 in the nude mouse. Anticancer Research, 17(6D), 4253-8.
Soos G, et al. Comparative Intraosseal Growth of Human Prostate Cancer Cell Lines LNCaP and PC-3 in the Nude Mouse. Anticancer Res. 1997 Nov-Dec;17(6D):4253-8. PubMed PMID: 9494517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative intraosseal growth of human prostate cancer cell lines LNCaP and PC-3 in the nude mouse. AU - Soos,G, AU - Jones,R F, AU - Haas,G P, AU - Wang,C Y, PY - 1998/3/12/pubmed PY - 1998/3/12/medline PY - 1998/3/12/entrez SP - 4253 EP - 8 JF - Anticancer research JO - Anticancer Res VL - 17 IS - 6D N2 - BACKGROUND: More than 75% of patients with advanced prostate carcinoma have skeletal involvement, which is the principal metastatic site and the major complication of this disease. The goal of this work was to compare the osseous metastasis of androgen-sensitive and insensitive prostate cancers in the nude mouse. MATERIALS AND METHODS: Androgen-sensitive LNCaP or -insensitive PC-3 human prostate carcinoma cells were injected directly into the femur medullas of male nude Beige mice, the animals were then sacrificed at successive time intervals to study the gross and microscopic characteristics of the established tumors. RESULTS: LNCaP and PC-3 both colonized in the bone marrow within a week, then gradually expanded to the entire bone medulla followed by osseous infiltration to produce obvious symptoms in the affected extremities. Based on the morphology, both osteoblastic and osteolytic changes occurred during the course of tumor progression. In addition, PC-3 tumors eventually broke through the bone cortex, invaded the surrounding tissues, and metastasized to the regional lymph nodes. In contrast, LNCaP remained localized within the bone, and appeared to eventually regress and die after displacing the normal bone marrow cells. Immunohistochemically, LNCaP tumors were consistently positive for prostate-specific antigen in bone metastasis, while PC-3 tumors were negative. Tumor cell nuclei of both PC-3 and LNCaP hybridized to a human repeated sequence DNA probe indicating that the proliferating malignant cells were of human origin. CONCLUSIONS: These cancer cell lines produced a high incidence of growth in the bone that differed in histogenesis. The relative malignancy of these cell lines was demonstrated in this model. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/9494517/Comparative_intraosseal_growth_of_human_prostate_cancer_cell_lines_LNCaP_and_PC_3_in_the_nude_mouse_ L2 - http://www.diseaseinfosearch.org/result/9175 DB - PRIME DP - Unbound Medicine ER -