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Identification of common allergenic structures in mugwort and ragweed pollen.
J Allergy Clin Immunol. 1998 Feb; 101(2 Pt 1):196-206.JA

Abstract

Identification of common allergenic structures in mugwort and ragweed pollen.

BACKGROUND

Despite the rare occurrence of ragweed in Middle Europe, a surprisingly high number of patients allergic to mugwort, a frequently encountered weed, display IgE reactivity against ragweed pollen allergens.

OBJECTIVE

The aim of this study was to investigate whether the high prevalence of IgE reactivity against ragweed in patients allergic to mugwort is caused by the presence of common allergenic determinants. We also sought to characterize any cross-reactive allergens.

METHODS

Common allergenic structures in mugwort and ragweed pollen were characterized by qualitative IgE immunoblot inhibition experiments performed with natural allergen extracts and recombinant allergens. The degree of cross-reactivity was estimated by quantitative CAP-FEIA competitions. The clinical significance of cross-reactive IgE antibodies was studied with histamine release experiments and nasal provocation tests.

RESULTS

Mugwort and ragweed RAST values were significantly correlated in a population of 82 Austrian patients allergic to mugwort. IgE antibodies cross-reacted with allergens of comparable molecular weight that were present in both extracts. By using recombinant birch profilin and specific antisera for IgE inhibition experiments, profilin was identified as one of the cross-reactive components in mugwort and ragweed pollen. Preincubation of sera from patients allergic to mugwort with mugwort extract inhibited IgE binding to ragweed pollen extract greater than 80%. Mugwort and ragweed pollen extract induced comparable histamine release and reduction of nasal air flow in a patient with IgE reactivity against the major mugwort allergen Art v 1.

CONCLUSION

In addition to profilin, mugwort and ragweed pollen contain a number of cross-reactive allergens, among them the major mugwort allergen Art v 1. Cross-reactive IgE antibodies can lead to clinically significant allergic reactions.

Authors+Show Affiliations

Institute of General and Experimental Pathology, AKH, University of Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9500752

Citation

Hirschwehr, R, et al. "Identification of Common Allergenic Structures in Mugwort and Ragweed Pollen." The Journal of Allergy and Clinical Immunology, vol. 101, no. 2 Pt 1, 1998, pp. 196-206.
Hirschwehr R, Heppner C, Spitzauer S, et al. Identification of common allergenic structures in mugwort and ragweed pollen. J Allergy Clin Immunol. 1998;101(2 Pt 1):196-206.
Hirschwehr, R., Heppner, C., Spitzauer, S., Sperr, W. R., Valent, P., Berger, U., Horak, F., Jäger, S., Kraft, D., & Valenta, R. (1998). Identification of common allergenic structures in mugwort and ragweed pollen. The Journal of Allergy and Clinical Immunology, 101(2 Pt 1), 196-206.
Hirschwehr R, et al. Identification of Common Allergenic Structures in Mugwort and Ragweed Pollen. J Allergy Clin Immunol. 1998;101(2 Pt 1):196-206. PubMed PMID: 9500752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of common allergenic structures in mugwort and ragweed pollen. AU - Hirschwehr,R, AU - Heppner,C, AU - Spitzauer,S, AU - Sperr,W R, AU - Valent,P, AU - Berger,U, AU - Horak,F, AU - Jäger,S, AU - Kraft,D, AU - Valenta,R, PY - 1998/3/21/pubmed PY - 1998/3/21/medline PY - 1998/3/21/entrez SP - 196 EP - 206 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 101 IS - 2 Pt 1 N2 - UNLABELLED: Identification of common allergenic structures in mugwort and ragweed pollen. BACKGROUND: Despite the rare occurrence of ragweed in Middle Europe, a surprisingly high number of patients allergic to mugwort, a frequently encountered weed, display IgE reactivity against ragweed pollen allergens. OBJECTIVE: The aim of this study was to investigate whether the high prevalence of IgE reactivity against ragweed in patients allergic to mugwort is caused by the presence of common allergenic determinants. We also sought to characterize any cross-reactive allergens. METHODS: Common allergenic structures in mugwort and ragweed pollen were characterized by qualitative IgE immunoblot inhibition experiments performed with natural allergen extracts and recombinant allergens. The degree of cross-reactivity was estimated by quantitative CAP-FEIA competitions. The clinical significance of cross-reactive IgE antibodies was studied with histamine release experiments and nasal provocation tests. RESULTS: Mugwort and ragweed RAST values were significantly correlated in a population of 82 Austrian patients allergic to mugwort. IgE antibodies cross-reacted with allergens of comparable molecular weight that were present in both extracts. By using recombinant birch profilin and specific antisera for IgE inhibition experiments, profilin was identified as one of the cross-reactive components in mugwort and ragweed pollen. Preincubation of sera from patients allergic to mugwort with mugwort extract inhibited IgE binding to ragweed pollen extract greater than 80%. Mugwort and ragweed pollen extract induced comparable histamine release and reduction of nasal air flow in a patient with IgE reactivity against the major mugwort allergen Art v 1. CONCLUSION: In addition to profilin, mugwort and ragweed pollen contain a number of cross-reactive allergens, among them the major mugwort allergen Art v 1. Cross-reactive IgE antibodies can lead to clinically significant allergic reactions. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/9500752/Identification_of_common_allergenic_structures_in_mugwort_and_ragweed_pollen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(98)70384-7 DB - PRIME DP - Unbound Medicine ER -