Tags

Type your tag names separated by a space and hit enter

Carnitine palmitoyltransferase II activity is decreased in liver mitochondria of cachectic rats bearing the Walker 256 carcinosarcoma: effect of indomethacin treatment.
Biochem Mol Biol Int. 1998 Jan; 44(1):185-93.BM

Abstract

The syndrome of cancer cachexia is accompanied by several alterations of lipid metabolism, especially that in the liver. In this study we have investigated a possible mechanism whereby the presence of the Walker 256 carcinosarcoma affects hepatic fatty acid oxidative capacity in tumour-bearing rats. Hepatic mitochondrial outer membrane carnitine palmitoyltransferase I (CPT I), generally accepted as the main site of regulation of fatty acid oxidation, was unaffected by the presence of the extra-hepatic tumour. However, mitochondrial inner-membrane carnitine palmitoyltransferase II (CPT II) activity was markedly decreased in mitochondria isolated from the liver of tumour-bearing rats. Immuno-detection by Western blotting using a CPT II-specific antibody identified two bands (corresponding to M(r) 69,000 and 54,000) in tumour-bearing rats whereas only the normal-sized CPT II was present (at the expected M(r) 69,000) in mitochondria from control rats. It is suggested that the emergence of the second, smaller protein may represent a catalytically less active protein that arises in vivo, since its appearance was not affected by the inclusion of proteolysis inhibitors in the mitochondrial preparation buffers. Treatment of the tumour-bearing rats with indomethacin, a prostaglandin (including PGE2) synthesis inhibitor, increased CPT II activity to levels even higher than those found in the control animals. It is suggested that PGE2 may play a role in the control of CPT II expression in the liver of tumour-bearing rats. Indomethacin treatment did not affect either of the two CPT activities of the mitochondria isolated from tumour tissue.

Authors+Show Affiliations

Departamento de Histologia e Embryologia, Cidade Universitária, São Paulo, Brasil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9503162

Citation

Seelaender, M C., et al. "Carnitine Palmitoyltransferase II Activity Is Decreased in Liver Mitochondria of Cachectic Rats Bearing the Walker 256 Carcinosarcoma: Effect of Indomethacin Treatment." Biochemistry and Molecular Biology International, vol. 44, no. 1, 1998, pp. 185-93.
Seelaender MC, Curi R, Colquhoun A, et al. Carnitine palmitoyltransferase II activity is decreased in liver mitochondria of cachectic rats bearing the Walker 256 carcinosarcoma: effect of indomethacin treatment. Biochem Mol Biol Int. 1998;44(1):185-93.
Seelaender, M. C., Curi, R., Colquhoun, A., Williams, J. F., & Zammitt, V. A. (1998). Carnitine palmitoyltransferase II activity is decreased in liver mitochondria of cachectic rats bearing the Walker 256 carcinosarcoma: effect of indomethacin treatment. Biochemistry and Molecular Biology International, 44(1), 185-93.
Seelaender MC, et al. Carnitine Palmitoyltransferase II Activity Is Decreased in Liver Mitochondria of Cachectic Rats Bearing the Walker 256 Carcinosarcoma: Effect of Indomethacin Treatment. Biochem Mol Biol Int. 1998;44(1):185-93. PubMed PMID: 9503162.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carnitine palmitoyltransferase II activity is decreased in liver mitochondria of cachectic rats bearing the Walker 256 carcinosarcoma: effect of indomethacin treatment. AU - Seelaender,M C, AU - Curi,R, AU - Colquhoun,A, AU - Williams,J F, AU - Zammitt,V A, PY - 1998/3/21/pubmed PY - 1998/3/21/medline PY - 1998/3/21/entrez SP - 185 EP - 93 JF - Biochemistry and molecular biology international JO - Biochem Mol Biol Int VL - 44 IS - 1 N2 - The syndrome of cancer cachexia is accompanied by several alterations of lipid metabolism, especially that in the liver. In this study we have investigated a possible mechanism whereby the presence of the Walker 256 carcinosarcoma affects hepatic fatty acid oxidative capacity in tumour-bearing rats. Hepatic mitochondrial outer membrane carnitine palmitoyltransferase I (CPT I), generally accepted as the main site of regulation of fatty acid oxidation, was unaffected by the presence of the extra-hepatic tumour. However, mitochondrial inner-membrane carnitine palmitoyltransferase II (CPT II) activity was markedly decreased in mitochondria isolated from the liver of tumour-bearing rats. Immuno-detection by Western blotting using a CPT II-specific antibody identified two bands (corresponding to M(r) 69,000 and 54,000) in tumour-bearing rats whereas only the normal-sized CPT II was present (at the expected M(r) 69,000) in mitochondria from control rats. It is suggested that the emergence of the second, smaller protein may represent a catalytically less active protein that arises in vivo, since its appearance was not affected by the inclusion of proteolysis inhibitors in the mitochondrial preparation buffers. Treatment of the tumour-bearing rats with indomethacin, a prostaglandin (including PGE2) synthesis inhibitor, increased CPT II activity to levels even higher than those found in the control animals. It is suggested that PGE2 may play a role in the control of CPT II expression in the liver of tumour-bearing rats. Indomethacin treatment did not affect either of the two CPT activities of the mitochondria isolated from tumour tissue. SN - 1039-9712 UR - https://www.unboundmedicine.com/medline/citation/9503162/Carnitine_palmitoyltransferase_II_activity_is_decreased_in_liver_mitochondria_of_cachectic_rats_bearing_the_Walker_256_carcinosarcoma:_effect_of_indomethacin_treatment_ DB - PRIME DP - Unbound Medicine ER -