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Two-hybrid systematic screening of the yeast proteome.
Bioessays. 1998 Jan; 20(1):1-5.B

Abstract

The yeast two-hybrid system is a genetic method that detects protein-protein interactions. One application is the detection by library screening of new interactors of a protein of known function. In the August issue of Nature Genetics, Fromont-Racine et al. showed for the first time that the construction of the protein interaction map of a complex pathway, such as that of the mRNA splicing machinery, is now possible, because of the combination of recent technical improvements elaborated in several laboratories. With a yeast cell mating procedure that increases screen efficiency, they used their complex yeast genomic library of 5 x 10(6) clones to test 700 x 10(6) interactions against 15 proteins. They identified and classified 170 potential interactors, including approximately 70 proteins of previously unknown function. More than 25% of the interactors are probably biologically relevant. The achievements of Fromont-Racine et al. have opened the way to the systematic analysis of the protein interaction networks of the 6,000 open reading frames-yeast proteome. This task requires, however, automation of the library screens and creation of a two-hybrid library database.

Authors+Show Affiliations

Lecrenier N
Laboratoire de Biochimie Physiologique, Louvain-La-Neuve, Belgium.
Foury F
No affiliation info available
Goffeau A
No affiliation info available

MeSH

Fungal ProteinsGenetic TechniquesGenome, FungalGenomic LibraryHybridization, GeneticRNA PrecursorsRNA SplicingRNA, FungalSaccharomyces cerevisiae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9504041

Citation

Lecrenier, N, et al. "Two-hybrid Systematic Screening of the Yeast Proteome." BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology, vol. 20, no. 1, 1998, pp. 1-5.
Lecrenier N, Foury F, Goffeau A. Two-hybrid systematic screening of the yeast proteome. Bioessays. 1998;20(1):1-5.
Lecrenier, N., Foury, F., & Goffeau, A. (1998). Two-hybrid systematic screening of the yeast proteome. BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology, 20(1), 1-5.
Lecrenier N, Foury F, Goffeau A. Two-hybrid Systematic Screening of the Yeast Proteome. Bioessays. 1998;20(1):1-5. PubMed PMID: 9504041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-hybrid systematic screening of the yeast proteome. AU - Lecrenier,N, AU - Foury,F, AU - Goffeau,A, PY - 1998/3/21/pubmed PY - 2000/6/20/medline PY - 1998/3/21/entrez SP - 1 EP - 5 JF - BioEssays : news and reviews in molecular, cellular and developmental biology JO - Bioessays VL - 20 IS - 1 N2 - The yeast two-hybrid system is a genetic method that detects protein-protein interactions. One application is the detection by library screening of new interactors of a protein of known function. In the August issue of Nature Genetics, Fromont-Racine et al. showed for the first time that the construction of the protein interaction map of a complex pathway, such as that of the mRNA splicing machinery, is now possible, because of the combination of recent technical improvements elaborated in several laboratories. With a yeast cell mating procedure that increases screen efficiency, they used their complex yeast genomic library of 5 x 10(6) clones to test 700 x 10(6) interactions against 15 proteins. They identified and classified 170 potential interactors, including approximately 70 proteins of previously unknown function. More than 25% of the interactors are probably biologically relevant. The achievements of Fromont-Racine et al. have opened the way to the systematic analysis of the protein interaction networks of the 6,000 open reading frames-yeast proteome. This task requires, however, automation of the library screens and creation of a two-hybrid library database. SN - 0265-9247 UR - https://www.unboundmedicine.com/medline/citation/9504041/Two_hybrid_systematic_screening_of_the_yeast_proteome_ L2 - https://doi.org/10.1002/(SICI)1521-1878(199801)20:1<1::AID-BIES2>3.0.CO;2-Y DB - PRIME DP - Unbound Medicine ER -