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Comparison of the antioxidant activity of melatonin and pinoline in vitro.
J Pineal Res. 1998 Mar; 24(2):96-101.JP

Abstract

Several recent experiments have shown that melatonin is an efficient antioxidant and free radical scavenger. In the present study the antioxidative effect of melatonin was compared with that of pinoline. Pinoline (6-methoxy-tetrahydro-beta-carboline) can be formed in the mammalian body under physiological conditions from 5-hydroxytryptamine or as a tricyclic metabolite of melatonin. Both melatonin and pinoline inhibited lipid peroxidation and showed comparable activity in a total antioxidant status test. Melatonin and pinoline concentration-dependently scavenged hydroxyl radicals with IC50 11.4+/-1.0 microM for melatonin and 62.3+/-3.8 microM for pinoline. These results support the importance of the indolic part of the molecule and the 5-methoxy group common to both compounds in terms of the ability of these molecules to quench the hydroxyl radicals. As pinoline has been shown to exert an antidepressant-like effect in behavioral experiments and has been reported to have a low toxicity, this compound should be further studied as a potential antidepressant with pronounced antioxidative effects. These results further support the importance of pineal gland in antioxidative protection.

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Authors+Show Affiliations

Pähkla R
Department of Pharmacology, University of Tartu, Estonia. rein@sam.ee
Zilmer M
No affiliation info available
Kullisaar T
No affiliation info available
Rägo L
No affiliation info available

MeSH

AntioxidantsCarbolinesDose-Response Relationship, DrugFree Radical ScavengersHydroxyl RadicalIn Vitro TechniquesLipid PeroxidationMelatoninalpha-Linolenic Acid

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9510434

Citation

Pähkla, R, et al. "Comparison of the Antioxidant Activity of Melatonin and Pinoline in Vitro." Journal of Pineal Research, vol. 24, no. 2, 1998, pp. 96-101.
Pähkla R, Zilmer M, Kullisaar T, et al. Comparison of the antioxidant activity of melatonin and pinoline in vitro. J Pineal Res. 1998;24(2):96-101.
Pähkla, R., Zilmer, M., Kullisaar, T., & Rägo, L. (1998). Comparison of the antioxidant activity of melatonin and pinoline in vitro. Journal of Pineal Research, 24(2), 96-101.
Pähkla R, et al. Comparison of the Antioxidant Activity of Melatonin and Pinoline in Vitro. J Pineal Res. 1998;24(2):96-101. PubMed PMID: 9510434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the antioxidant activity of melatonin and pinoline in vitro. AU - Pähkla,R, AU - Zilmer,M, AU - Kullisaar,T, AU - Rägo,L, PY - 1998/3/24/pubmed PY - 1998/3/24/medline PY - 1998/3/24/entrez SP - 96 EP - 101 JF - Journal of pineal research JO - J Pineal Res VL - 24 IS - 2 N2 - Several recent experiments have shown that melatonin is an efficient antioxidant and free radical scavenger. In the present study the antioxidative effect of melatonin was compared with that of pinoline. Pinoline (6-methoxy-tetrahydro-beta-carboline) can be formed in the mammalian body under physiological conditions from 5-hydroxytryptamine or as a tricyclic metabolite of melatonin. Both melatonin and pinoline inhibited lipid peroxidation and showed comparable activity in a total antioxidant status test. Melatonin and pinoline concentration-dependently scavenged hydroxyl radicals with IC50 11.4+/-1.0 microM for melatonin and 62.3+/-3.8 microM for pinoline. These results support the importance of the indolic part of the molecule and the 5-methoxy group common to both compounds in terms of the ability of these molecules to quench the hydroxyl radicals. As pinoline has been shown to exert an antidepressant-like effect in behavioral experiments and has been reported to have a low toxicity, this compound should be further studied as a potential antidepressant with pronounced antioxidative effects. These results further support the importance of pineal gland in antioxidative protection. SN - 0742-3098 UR - https://www.unboundmedicine.com/medline/citation/9510434/Comparison_of_the_antioxidant_activity_of_melatonin_and_pinoline_in_vitro_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0742-3098&date=1998&volume=24&issue=2&spage=96 DB - PRIME DP - Unbound Medicine ER -