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Improved protamine-sensitive membrane electrode for monitoring heparin concentrations in whole blood via protamine titration.
Clin Chem 1998; 44(3):606-13CC

Abstract

An improved protamine-sensitive electrode based on a polymeric membrane doped with the charged ion exchanger dinonylnaphthalenesulfonate (DNNS) is used for monitoring heparin concentrations in whole blood. The electrode exhibits significant nonequilibrium potentiometric response to polycationic protamine over the concentration range of 0.5-20 mg/L in undiluted whole-blood samples. The sensor can serve as a simple end point detector for the determination of heparin via potentiometric titrations with protamine. Whole-blood heparin concentrations determined by the electrode method (n > or = 157) correlate well with other protamine titration-based methods, including the commercial Hepcon HMS assay (r = 0.934) and a previously reported potentiometric heparin sensor-based method (r = 0.973). Reasonable correlation was also found with a commercial chromogenic anti-Xa heparin assay (r = 0.891) with corresponding plasma samples and appropriate correction for whole-blood hematocrit levels. Whereas a significant positive bias (0.62 kU/L; P < 0.001) is observed between the anti-Xa assay and the protamine sensor methods, insignificant bias is observed between the protamine sensor and the Hepcon HMS tests (0.08 kU/L; P = 0.02). The possibility of fully automating these titrations offers a potentially simple, inexpensive, and accurate method for monitoring heparin concentrations in whole blood.

Authors+Show Affiliations

Department of Chemistry, College of Pharmacy, University of Michigan, Ann Arbor 48109, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9510869

Citation

Ramamurthy, N, et al. "Improved Protamine-sensitive Membrane Electrode for Monitoring Heparin Concentrations in Whole Blood Via Protamine Titration." Clinical Chemistry, vol. 44, no. 3, 1998, pp. 606-13.
Ramamurthy N, Baliga N, Wahr JA, et al. Improved protamine-sensitive membrane electrode for monitoring heparin concentrations in whole blood via protamine titration. Clin Chem. 1998;44(3):606-13.
Ramamurthy, N., Baliga, N., Wahr, J. A., Schaller, U., Yang, V. C., & Meyerhoff, M. E. (1998). Improved protamine-sensitive membrane electrode for monitoring heparin concentrations in whole blood via protamine titration. Clinical Chemistry, 44(3), pp. 606-13.
Ramamurthy N, et al. Improved Protamine-sensitive Membrane Electrode for Monitoring Heparin Concentrations in Whole Blood Via Protamine Titration. Clin Chem. 1998;44(3):606-13. PubMed PMID: 9510869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved protamine-sensitive membrane electrode for monitoring heparin concentrations in whole blood via protamine titration. AU - Ramamurthy,N, AU - Baliga,N, AU - Wahr,J A, AU - Schaller,U, AU - Yang,V C, AU - Meyerhoff,M E, PY - 1998/3/25/pubmed PY - 1998/3/25/medline PY - 1998/3/25/entrez SP - 606 EP - 13 JF - Clinical chemistry JO - Clin. Chem. VL - 44 IS - 3 N2 - An improved protamine-sensitive electrode based on a polymeric membrane doped with the charged ion exchanger dinonylnaphthalenesulfonate (DNNS) is used for monitoring heparin concentrations in whole blood. The electrode exhibits significant nonequilibrium potentiometric response to polycationic protamine over the concentration range of 0.5-20 mg/L in undiluted whole-blood samples. The sensor can serve as a simple end point detector for the determination of heparin via potentiometric titrations with protamine. Whole-blood heparin concentrations determined by the electrode method (n > or = 157) correlate well with other protamine titration-based methods, including the commercial Hepcon HMS assay (r = 0.934) and a previously reported potentiometric heparin sensor-based method (r = 0.973). Reasonable correlation was also found with a commercial chromogenic anti-Xa heparin assay (r = 0.891) with corresponding plasma samples and appropriate correction for whole-blood hematocrit levels. Whereas a significant positive bias (0.62 kU/L; P < 0.001) is observed between the anti-Xa assay and the protamine sensor methods, insignificant bias is observed between the protamine sensor and the Hepcon HMS tests (0.08 kU/L; P = 0.02). The possibility of fully automating these titrations offers a potentially simple, inexpensive, and accurate method for monitoring heparin concentrations in whole blood. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/9510869/Improved_protamine_sensitive_membrane_electrode_for_monitoring_heparin_concentrations_in_whole_blood_via_protamine_titration_ L2 - http://www.clinchem.org/cgi/pmidlookup?view=long&amp;pmid=9510869 DB - PRIME DP - Unbound Medicine ER -