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The analysis of fountain pen inks by capillary electrophoresis with ultraviolet/visible absorbance and laser-induced fluorescence detection.
Electrophoresis. 1998 Jan; 19(1):31-41.E

Abstract

Information on the identity of inks adds to the circumstantial evidence in legal cases involving fraudulent documents. In combination with optical methods, multiple thin-layer chromatography (TLC) is currently the analytical tool used by forensic chemists to separate, compare and distinguish inks based on their dye composition. In our studies, capillary electrophoresis (CE) was used for the analysis of water-soluble fountain pen inks. Inks are complex mixtures of synthetic organic and inorganic dyes, surfactants, resins and other components. The investigations included the development of an electrophoretic separation method, the optimization of an extraction procedure for inks from paper as well as the evaluation of ultraviolet/visible (UV/VIS) absorbance and laser-induced fluorescence (LIF) detection for the analysis of inks by CE. Good results for the separation of 17 blue and black inks of different manufacturers and countries of origin were obtained with 100 mM borate buffer, pH 8.0, containing 20% methanol. The electropherograms of the inks and their extracts from paper showed patterns that were in most cases distinctly different from each other. Ultraviolet/visible scans can be used to compare spectra of the separated main and trace components of inks. Fluorescence detection at different excitation and emission wavelengths was more sensitive, but added to the complexity of the electropherograms due to the excitation of coextracted fluorescing paper components. The resolution power of CE combined with the information content provided by the detection modes investigated prove CE to be a powerful tool for the identification of water-soluble inks used on paper documents.

Authors+Show Affiliations

Department of Chemistry, University of Cincinnati, OH 45221-0172, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

9511860

Citation

Rohde, E, et al. "The Analysis of Fountain Pen Inks By Capillary Electrophoresis With Ultraviolet/visible Absorbance and Laser-induced Fluorescence Detection." Electrophoresis, vol. 19, no. 1, 1998, pp. 31-41.
Rohde E, Vogt C, Heineman WR. The analysis of fountain pen inks by capillary electrophoresis with ultraviolet/visible absorbance and laser-induced fluorescence detection. Electrophoresis. 1998;19(1):31-41.
Rohde, E., Vogt, C., & Heineman, W. R. (1998). The analysis of fountain pen inks by capillary electrophoresis with ultraviolet/visible absorbance and laser-induced fluorescence detection. Electrophoresis, 19(1), 31-41.
Rohde E, Vogt C, Heineman WR. The Analysis of Fountain Pen Inks By Capillary Electrophoresis With Ultraviolet/visible Absorbance and Laser-induced Fluorescence Detection. Electrophoresis. 1998;19(1):31-41. PubMed PMID: 9511860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The analysis of fountain pen inks by capillary electrophoresis with ultraviolet/visible absorbance and laser-induced fluorescence detection. AU - Rohde,E, AU - Vogt,C, AU - Heineman,W R, PY - 1998/3/25/pubmed PY - 1998/3/25/medline PY - 1998/3/25/entrez SP - 31 EP - 41 JF - Electrophoresis JO - Electrophoresis VL - 19 IS - 1 N2 - Information on the identity of inks adds to the circumstantial evidence in legal cases involving fraudulent documents. In combination with optical methods, multiple thin-layer chromatography (TLC) is currently the analytical tool used by forensic chemists to separate, compare and distinguish inks based on their dye composition. In our studies, capillary electrophoresis (CE) was used for the analysis of water-soluble fountain pen inks. Inks are complex mixtures of synthetic organic and inorganic dyes, surfactants, resins and other components. The investigations included the development of an electrophoretic separation method, the optimization of an extraction procedure for inks from paper as well as the evaluation of ultraviolet/visible (UV/VIS) absorbance and laser-induced fluorescence (LIF) detection for the analysis of inks by CE. Good results for the separation of 17 blue and black inks of different manufacturers and countries of origin were obtained with 100 mM borate buffer, pH 8.0, containing 20% methanol. The electropherograms of the inks and their extracts from paper showed patterns that were in most cases distinctly different from each other. Ultraviolet/visible scans can be used to compare spectra of the separated main and trace components of inks. Fluorescence detection at different excitation and emission wavelengths was more sensitive, but added to the complexity of the electropherograms due to the excitation of coextracted fluorescing paper components. The resolution power of CE combined with the information content provided by the detection modes investigated prove CE to be a powerful tool for the identification of water-soluble inks used on paper documents. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/9511860/The_analysis_of_fountain_pen_inks_by_capillary_electrophoresis_with_ultraviolet/visible_absorbance_and_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.1150190108 DB - PRIME DP - Unbound Medicine ER -