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IL-6 and IL-8 production by human bone marrow stromal cells.
Cytokine. 1998 Feb; 10(2):93-7.C

Abstract

By using a specific enzyme-linked immunosorbent assay, the authors demonstrated that human bone marrow stromal cells produce IL-6 and IL-8. Their synthesis is enhanced in a dose-dependent manner after stimulation with lipopolysaccharide (LPS) and phorbol myristate acetate (PMA). Interleukin 6 (IL-6) and IL-8 production in response to PMA were markedly diminished by the PKC inhibitor staurosporine. IL-6 (10 ng/ml) stimulated IL-8 production with 0% and 10% fetal calf serum (FCS) in the culture medium. In similar conditions, IL-8 (10 ng/ml) enhanced IL-6 production. IL-1 alpha, IL-1 beta, and IL-3, tumour necrosis factor alpha (TNF-alpha), Stem cell factor (SCF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) (at 10 ng/ml) stimulated IL-6 and IL-8 production in 0% and 10% FCS. G-CSF stimulated and IL-4 inhibited IL-8 production in 10% FCS. IL-2, IL-4 and bFGF stimulated IL-6 production in 0% FCS. These results suggest that bone marrow stromal cells might represent a major source for the cytokine-regulated local production of IL-6 and IL-8 inside human bone marrow.

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Authors+Show Affiliations

Rougier F
Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, Limoges, France.
Cornu E
No affiliation info available
Praloran V
No affiliation info available
Denizot Y
No affiliation info available

MeSH

Bone Marrow CellsHumansInterleukin-6Interleukin-8LipopolysaccharidesMitogensStromal CellsTetradecanoylphorbol Acetate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9512898

Citation

Rougier, F, et al. "IL-6 and IL-8 Production By Human Bone Marrow Stromal Cells." Cytokine, vol. 10, no. 2, 1998, pp. 93-7.
Rougier F, Cornu E, Praloran V, et al. IL-6 and IL-8 production by human bone marrow stromal cells. Cytokine. 1998;10(2):93-7.
Rougier, F., Cornu, E., Praloran, V., & Denizot, Y. (1998). IL-6 and IL-8 production by human bone marrow stromal cells. Cytokine, 10(2), 93-7.
Rougier F, et al. IL-6 and IL-8 Production By Human Bone Marrow Stromal Cells. Cytokine. 1998;10(2):93-7. PubMed PMID: 9512898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-6 and IL-8 production by human bone marrow stromal cells. AU - Rougier,F, AU - Cornu,E, AU - Praloran,V, AU - Denizot,Y, PY - 1998/3/26/pubmed PY - 1998/3/26/medline PY - 1998/3/26/entrez SP - 93 EP - 7 JF - Cytokine JO - Cytokine VL - 10 IS - 2 N2 - By using a specific enzyme-linked immunosorbent assay, the authors demonstrated that human bone marrow stromal cells produce IL-6 and IL-8. Their synthesis is enhanced in a dose-dependent manner after stimulation with lipopolysaccharide (LPS) and phorbol myristate acetate (PMA). Interleukin 6 (IL-6) and IL-8 production in response to PMA were markedly diminished by the PKC inhibitor staurosporine. IL-6 (10 ng/ml) stimulated IL-8 production with 0% and 10% fetal calf serum (FCS) in the culture medium. In similar conditions, IL-8 (10 ng/ml) enhanced IL-6 production. IL-1 alpha, IL-1 beta, and IL-3, tumour necrosis factor alpha (TNF-alpha), Stem cell factor (SCF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) (at 10 ng/ml) stimulated IL-6 and IL-8 production in 0% and 10% FCS. G-CSF stimulated and IL-4 inhibited IL-8 production in 10% FCS. IL-2, IL-4 and bFGF stimulated IL-6 production in 0% FCS. These results suggest that bone marrow stromal cells might represent a major source for the cytokine-regulated local production of IL-6 and IL-8 inside human bone marrow. SN - 1043-4666 UR - https://www.unboundmedicine.com/medline/citation/9512898/IL_6_and_IL_8_production_by_human_bone_marrow_stromal_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-4666(97)90262-3 DB - PRIME DP - Unbound Medicine ER -