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The reaction of S-nitrosoglutathione with superoxide.
Biochem Biophys Res Commun. 1998 Mar 17; 244(2):525-30.BB

Abstract

The nitric oxide (NO)-dependent S-nitrosation of thiols to generate S-nitrosothiols has been proposed as an important pathway for the metabolism of NO in vivo. Although it has been suggested that these S-nitrosated compounds are resistant to decomposition by reactive oxygen metabolites (ROMs), very little information is available regarding the interaction between S-nitrosothiols and ROMs. We found that S-nitrosoglutathione (GSNO) rapidly reacted with O2- to generate glutathione disulfide and equimolar quantities of nitrite and nitrate. The reaction was second order with respect of GSNO and first order with respect of O2- with a rate equation of -d[GSNO]/dt = 2k3[GSNO]2[O2-], where k3 = 3 - 6 x 10(8) M-2s-1. In addition, the reaction of GSNO with O2- generated a strong oxidant as an intermediate capable of oxidizing dihydrorhodamine in the absence of the apparent generation of NO. We conclude that O2- may act as a physiological modulator of S-nitrosation reactions by directly promoting the decomposition of S-nitrosothiols.

Authors+Show Affiliations

Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport 71130, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9514894

Citation

Jourd'heuil, D, et al. "The Reaction of S-nitrosoglutathione With Superoxide." Biochemical and Biophysical Research Communications, vol. 244, no. 2, 1998, pp. 525-30.
Jourd'heuil D, Mai CT, Laroux FS, et al. The reaction of S-nitrosoglutathione with superoxide. Biochem Biophys Res Commun. 1998;244(2):525-30.
Jourd'heuil, D., Mai, C. T., Laroux, F. S., Wink, D. A., & Grisham, M. B. (1998). The reaction of S-nitrosoglutathione with superoxide. Biochemical and Biophysical Research Communications, 244(2), 525-30.
Jourd'heuil D, et al. The Reaction of S-nitrosoglutathione With Superoxide. Biochem Biophys Res Commun. 1998 Mar 17;244(2):525-30. PubMed PMID: 9514894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The reaction of S-nitrosoglutathione with superoxide. AU - Jourd'heuil,D, AU - Mai,C T, AU - Laroux,F S, AU - Wink,D A, AU - Grisham,M B, PY - 1998/3/26/pubmed PY - 1998/3/26/medline PY - 1998/3/26/entrez SP - 525 EP - 30 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 244 IS - 2 N2 - The nitric oxide (NO)-dependent S-nitrosation of thiols to generate S-nitrosothiols has been proposed as an important pathway for the metabolism of NO in vivo. Although it has been suggested that these S-nitrosated compounds are resistant to decomposition by reactive oxygen metabolites (ROMs), very little information is available regarding the interaction between S-nitrosothiols and ROMs. We found that S-nitrosoglutathione (GSNO) rapidly reacted with O2- to generate glutathione disulfide and equimolar quantities of nitrite and nitrate. The reaction was second order with respect of GSNO and first order with respect of O2- with a rate equation of -d[GSNO]/dt = 2k3[GSNO]2[O2-], where k3 = 3 - 6 x 10(8) M-2s-1. In addition, the reaction of GSNO with O2- generated a strong oxidant as an intermediate capable of oxidizing dihydrorhodamine in the absence of the apparent generation of NO. We conclude that O2- may act as a physiological modulator of S-nitrosation reactions by directly promoting the decomposition of S-nitrosothiols. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/9514894/The_reaction_of_S_nitrosoglutathione_with_superoxide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(98)98227-4 DB - PRIME DP - Unbound Medicine ER -