Tags

Type your tag names separated by a space and hit enter

The hyperthermophile chromosomal protein Sac7d sharply kinks DNA.
Nature. 1998 Mar 12; 392(6672):202-5.Nat

Abstract

The proteins Sac7d and Sso7d belong to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius and S. solfactaricus, respectively. These proteins are extremely stable to heat, acid and chemical agents. Sac7d binds to DNA without any particular sequence preference and thereby increases its melting temperature by approximately 40 degrees C. We have now solved and refined the crystal structure of Sac7d in complex with two DNA sequences to high resolution. The structures are examples of a nonspecific DNA-binding protein bound to DNA, and reveal that Sac7d binds in the minor groove, causing a sharp kinking of the DNA helix that is more marked than that induced by any sequence-specific DNA-binding proteins. The kink results from the intercalation of specific hydrophobic side chains of Sac7d into the DNA structure, but without causing any significant distortion of the protein structure relative to the uncomplexed protein in solution.

Authors+Show Affiliations

Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana 61801, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9515968

Citation

Robinson, H, et al. "The Hyperthermophile Chromosomal Protein Sac7d Sharply Kinks DNA." Nature, vol. 392, no. 6672, 1998, pp. 202-5.
Robinson H, Gao YG, McCrary BS, et al. The hyperthermophile chromosomal protein Sac7d sharply kinks DNA. Nature. 1998;392(6672):202-5.
Robinson, H., Gao, Y. G., McCrary, B. S., Edmondson, S. P., Shriver, J. W., & Wang, A. H. (1998). The hyperthermophile chromosomal protein Sac7d sharply kinks DNA. Nature, 392(6672), 202-5.
Robinson H, et al. The Hyperthermophile Chromosomal Protein Sac7d Sharply Kinks DNA. Nature. 1998 Mar 12;392(6672):202-5. PubMed PMID: 9515968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The hyperthermophile chromosomal protein Sac7d sharply kinks DNA. AU - Robinson,H, AU - Gao,Y G, AU - McCrary,B S, AU - Edmondson,S P, AU - Shriver,J W, AU - Wang,A H, PY - 1998/3/27/pubmed PY - 2001/3/23/medline PY - 1998/3/27/entrez SP - 202 EP - 5 JF - Nature JO - Nature VL - 392 IS - 6672 N2 - The proteins Sac7d and Sso7d belong to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius and S. solfactaricus, respectively. These proteins are extremely stable to heat, acid and chemical agents. Sac7d binds to DNA without any particular sequence preference and thereby increases its melting temperature by approximately 40 degrees C. We have now solved and refined the crystal structure of Sac7d in complex with two DNA sequences to high resolution. The structures are examples of a nonspecific DNA-binding protein bound to DNA, and reveal that Sac7d binds in the minor groove, causing a sharp kinking of the DNA helix that is more marked than that induced by any sequence-specific DNA-binding proteins. The kink results from the intercalation of specific hydrophobic side chains of Sac7d into the DNA structure, but without causing any significant distortion of the protein structure relative to the uncomplexed protein in solution. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/9515968/The_hyperthermophile_chromosomal_protein_Sac7d_sharply_kinks_DNA_ L2 - https://doi.org/10.1038/32455 DB - PRIME DP - Unbound Medicine ER -