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A prospective study of K-ras mutations in the plasma of pancreatic cancer patients.
Clin Cancer Res. 1998 Feb; 4(2):271-5.CC

Abstract

K-ras mutations are frequently found in primary pancreatic adenocarcinomas. In this prospective study, we looked for K-ras mutations in the plasma of patients with pancreatic cancer. We isolated plasma DNA from 21 pancreatic cancer patients using a simple and rapid extraction technique and detected K-ras alterations with a PCR assay and subsequent product sequencing. Patients were followed up to determine their clinical outcome. We found K-ras mutations in the plasma of 17 patients (81%). In cases in which both plasma and pancreatic tissue were available, DNA mutations were similar in corresponding plasma and tissue samples. Plasma DNA alterations were found 5-14 months before clinical diagnosis in four patients. Mutant DNA was not found in the plasma of two patients with chronic pancreatitis or in five healthy controls. Our results indicate that K-ras mutations are often found in DNA isolated from the plasma of pancreatic cancer patients and that a noninvasive plasma-based assay may provide qualitative diagnostic information to clinicians in the future. Larger studies are required to further assess the relevance of our findings to clinical practice.

Authors+Show Affiliations

Digestive Diseases Research Centre, St. Bartholomew's, London, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9516910

Citation

Mulcahy, H E., et al. "A Prospective Study of K-ras Mutations in the Plasma of Pancreatic Cancer Patients." Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, vol. 4, no. 2, 1998, pp. 271-5.
Mulcahy HE, Lyautey J, Lederrey C, et al. A prospective study of K-ras mutations in the plasma of pancreatic cancer patients. Clin Cancer Res. 1998;4(2):271-5.
Mulcahy, H. E., Lyautey, J., Lederrey, C., qi Chen, X., Anker, P., Alstead, E. M., Ballinger, A., Farthing, M. J., & Stroun, M. (1998). A prospective study of K-ras mutations in the plasma of pancreatic cancer patients. Clinical Cancer Research : an Official Journal of the American Association for Cancer Research, 4(2), 271-5.
Mulcahy HE, et al. A Prospective Study of K-ras Mutations in the Plasma of Pancreatic Cancer Patients. Clin Cancer Res. 1998;4(2):271-5. PubMed PMID: 9516910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A prospective study of K-ras mutations in the plasma of pancreatic cancer patients. AU - Mulcahy,H E, AU - Lyautey,J, AU - Lederrey,C, AU - qi Chen,X, AU - Anker,P, AU - Alstead,E M, AU - Ballinger,A, AU - Farthing,M J, AU - Stroun,M, PY - 1998/5/14/pubmed PY - 1998/5/14/medline PY - 1998/5/14/entrez SP - 271 EP - 5 JF - Clinical cancer research : an official journal of the American Association for Cancer Research JO - Clin Cancer Res VL - 4 IS - 2 N2 - K-ras mutations are frequently found in primary pancreatic adenocarcinomas. In this prospective study, we looked for K-ras mutations in the plasma of patients with pancreatic cancer. We isolated plasma DNA from 21 pancreatic cancer patients using a simple and rapid extraction technique and detected K-ras alterations with a PCR assay and subsequent product sequencing. Patients were followed up to determine their clinical outcome. We found K-ras mutations in the plasma of 17 patients (81%). In cases in which both plasma and pancreatic tissue were available, DNA mutations were similar in corresponding plasma and tissue samples. Plasma DNA alterations were found 5-14 months before clinical diagnosis in four patients. Mutant DNA was not found in the plasma of two patients with chronic pancreatitis or in five healthy controls. Our results indicate that K-ras mutations are often found in DNA isolated from the plasma of pancreatic cancer patients and that a noninvasive plasma-based assay may provide qualitative diagnostic information to clinicians in the future. Larger studies are required to further assess the relevance of our findings to clinical practice. SN - 1078-0432 UR - https://www.unboundmedicine.com/medline/citation/9516910/A_prospective_study_of_K_ras_mutations_in_the_plasma_of_pancreatic_cancer_patients_ L2 - http://clincancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=9516910 DB - PRIME DP - Unbound Medicine ER -