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Protection against glutamate neurotoxicity in retinal cultures by acidic conditions.
Jpn J Pharmacol. 1998 Jan; 76(1):87-95.JJ

Abstract

We evaluated the effects of extracellular acidic conditions on glutamate-induced death in cultured retinal neurons. Primary retinal cultures, obtained from 3- to 5-day-old Wistar rats, were estimated to be consisted of mainly amacrine cells (90%) together with a small population of horizontal (8%) and ganglion cells (2%). We examined the effects of acidic pH (pH 6.0 to 7.0) on glutamate neurotoxicity by monitoring the delayed death of retinal neurons induced by brief (10 min) exposure to 1 mM glutamate followed by a 24-hr incubation. The glutamate-induced delayed death of cultured retinal neurons was attenuated with an acidic pH between 6.0 and 7.0. Furthermore, whole-cell patch-clamp recordings were taken from retinal neurons to examine the effects of acidic pH on N-methyl-D-aspartate (NMDA) or kainate receptor-mediated currents. NMDA- and kainate-induced currents were suppressed at pH 6.0 to 7.0 and pH 6.0 to 6.5, respectively. The acidity of the medium protected the retinal neurons from glutamate-induced delayed death, probably by inhibiting NMDA and/or kainate receptor activation.

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Authors+Show Affiliations

Saitoh T
Department of Ophthalmology, Hiroshima University School of Medicine, Japan.
Mishima HK
No affiliation info available
Shoge K
No affiliation info available
Ishihara K
No affiliation info available
Sasa M
No affiliation info available

MeSH

6-Cyano-7-nitroquinoxaline-2,3-dioneAcidsAnimalsCell DeathCells, CulturedDizocilpine MaleateDose-Response Relationship, DrugExcitatory Amino Acid AntagonistsGlutamic AcidHydrogen-Ion ConcentrationImmunohistochemistryNeuronsPatch-Clamp TechniquesRatsRats, WistarReceptors, Kainic AcidReceptors, N-Methyl-D-AspartateRetinaRetinal Ganglion Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9517408

Citation

Saitoh, T, et al. "Protection Against Glutamate Neurotoxicity in Retinal Cultures By Acidic Conditions." Japanese Journal of Pharmacology, vol. 76, no. 1, 1998, pp. 87-95.
Saitoh T, Mishima HK, Shoge K, et al. Protection against glutamate neurotoxicity in retinal cultures by acidic conditions. Jpn J Pharmacol. 1998;76(1):87-95.
Saitoh, T., Mishima, H. K., Shoge, K., Ishihara, K., & Sasa, M. (1998). Protection against glutamate neurotoxicity in retinal cultures by acidic conditions. Japanese Journal of Pharmacology, 76(1), 87-95.
Saitoh T, et al. Protection Against Glutamate Neurotoxicity in Retinal Cultures By Acidic Conditions. Jpn J Pharmacol. 1998;76(1):87-95. PubMed PMID: 9517408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection against glutamate neurotoxicity in retinal cultures by acidic conditions. AU - Saitoh,T, AU - Mishima,H K, AU - Shoge,K, AU - Ishihara,K, AU - Sasa,M, PY - 1998/3/28/pubmed PY - 1998/3/28/medline PY - 1998/3/28/entrez SP - 87 EP - 95 JF - Japanese journal of pharmacology JO - Jpn J Pharmacol VL - 76 IS - 1 N2 - We evaluated the effects of extracellular acidic conditions on glutamate-induced death in cultured retinal neurons. Primary retinal cultures, obtained from 3- to 5-day-old Wistar rats, were estimated to be consisted of mainly amacrine cells (90%) together with a small population of horizontal (8%) and ganglion cells (2%). We examined the effects of acidic pH (pH 6.0 to 7.0) on glutamate neurotoxicity by monitoring the delayed death of retinal neurons induced by brief (10 min) exposure to 1 mM glutamate followed by a 24-hr incubation. The glutamate-induced delayed death of cultured retinal neurons was attenuated with an acidic pH between 6.0 and 7.0. Furthermore, whole-cell patch-clamp recordings were taken from retinal neurons to examine the effects of acidic pH on N-methyl-D-aspartate (NMDA) or kainate receptor-mediated currents. NMDA- and kainate-induced currents were suppressed at pH 6.0 to 7.0 and pH 6.0 to 6.5, respectively. The acidity of the medium protected the retinal neurons from glutamate-induced delayed death, probably by inhibiting NMDA and/or kainate receptor activation. SN - 0021-5198 UR - https://www.unboundmedicine.com/medline/citation/9517408/Protection_against_glutamate_neurotoxicity_in_retinal_cultures_by_acidic_conditions_ L2 - https://joi.jlc.jst.go.jp/JST.JSTAGE/jjp/76.87?from=PubMed DB - PRIME DP - Unbound Medicine ER -