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Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues.
J Bone Miner Res 1998; 13(3):422-31JB

Abstract

Dentin matrix protein 1 (Dmp1) is an acidic phosphoprotein first identified by cDNA cloning from a rat tooth library. Northern blot hybridization of a variety of tissues detected Dmp1 mRNAs only in odontoblasts, suggesting that this protein was odontoblast specific. In situ hybridization studies showed expression of Dmp1 in odontoblasts with transient expression in secretory ameloblasts. The purpose of this study was to isolate and characterize a mouse Dmp1 cDNA and determine its spatial expression pattern related to other mineralizing tissues. A mouse molar cDNA library was screened with a 32P-labeled Dmp1 polymerase chain reaction amplification product in order to isolate a full-length clone. DNA sequence analysis of the largest mouse Dmp1 cDNA (2802 base pairs [bp]) revealed an open reading frame of 1509 nucleotides encoding a 503 amino acid protein with a single polyadenylation signal. Comparison with rat and bovine Dmp1 sequence showed high homology and the identification of a 45 bp (15 amino acid) insert, representing an alternative spliced mRNA. This 45 bp segment was shown to represent a small exon by DNA analysis of a mouse genomic Dmp1 clone. In situ hybridization studies revealed a much broader Dmp1 tissue expression pattern than previously reported. Dmp1 transcripts were detected in the odontoblast and ameloblasts, osteoblasts, and cementoblasts. Our data indicate that Dmp1 is alternatively spliced, and the primary full-length transcript contains a 45 bp insert which is encoded by a small exon. Therefore, Dmp1 is not a tooth-specific protein but rather is expressed in a number of mineralizing tissues including enamel, bone, and cementum.

Authors+Show Affiliations

Department of Pediatric Dentistry, Dental School, University of Texas Health Science Center at San Antonio, 78284-7888, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9525343

Citation

MacDougall, M, et al. "Identification of a Novel Isoform of Mouse Dentin Matrix Protein 1: Spatial Expression in Mineralized Tissues." Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research, vol. 13, no. 3, 1998, pp. 422-31.
MacDougall M, Gu TT, Luan X, et al. Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues. J Bone Miner Res. 1998;13(3):422-31.
MacDougall, M., Gu, T. T., Luan, X., Simmons, D., & Chen, J. (1998). Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues. Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research, 13(3), pp. 422-31.
MacDougall M, et al. Identification of a Novel Isoform of Mouse Dentin Matrix Protein 1: Spatial Expression in Mineralized Tissues. J Bone Miner Res. 1998;13(3):422-31. PubMed PMID: 9525343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues. AU - MacDougall,M, AU - Gu,T T, AU - Luan,X, AU - Simmons,D, AU - Chen,J, PY - 1998/4/3/pubmed PY - 1998/4/3/medline PY - 1998/4/3/entrez SP - 422 EP - 31 JF - Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research JO - J. Bone Miner. Res. VL - 13 IS - 3 N2 - Dentin matrix protein 1 (Dmp1) is an acidic phosphoprotein first identified by cDNA cloning from a rat tooth library. Northern blot hybridization of a variety of tissues detected Dmp1 mRNAs only in odontoblasts, suggesting that this protein was odontoblast specific. In situ hybridization studies showed expression of Dmp1 in odontoblasts with transient expression in secretory ameloblasts. The purpose of this study was to isolate and characterize a mouse Dmp1 cDNA and determine its spatial expression pattern related to other mineralizing tissues. A mouse molar cDNA library was screened with a 32P-labeled Dmp1 polymerase chain reaction amplification product in order to isolate a full-length clone. DNA sequence analysis of the largest mouse Dmp1 cDNA (2802 base pairs [bp]) revealed an open reading frame of 1509 nucleotides encoding a 503 amino acid protein with a single polyadenylation signal. Comparison with rat and bovine Dmp1 sequence showed high homology and the identification of a 45 bp (15 amino acid) insert, representing an alternative spliced mRNA. This 45 bp segment was shown to represent a small exon by DNA analysis of a mouse genomic Dmp1 clone. In situ hybridization studies revealed a much broader Dmp1 tissue expression pattern than previously reported. Dmp1 transcripts were detected in the odontoblast and ameloblasts, osteoblasts, and cementoblasts. Our data indicate that Dmp1 is alternatively spliced, and the primary full-length transcript contains a 45 bp insert which is encoded by a small exon. Therefore, Dmp1 is not a tooth-specific protein but rather is expressed in a number of mineralizing tissues including enamel, bone, and cementum. SN - 0884-0431 UR - https://www.unboundmedicine.com/medline/citation/9525343/Identification_of_a_novel_isoform_of_mouse_dentin_matrix_protein_1:_spatial_expression_in_mineralized_tissues_ L2 - https://doi.org/10.1359/jbmr.1998.13.3.422 DB - PRIME DP - Unbound Medicine ER -