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[Species identification of intestinal microsporidiosis in HIV-positive patients using the polymerase chain reaction].
Enferm Infecc Microbiol Clin. 1997 Nov; 15(9):456-61.EI

Abstract

BACKGROUND

Microsporidia are opportunistic parasites which, due to their morphologic characteristics, continue presenting diagnostic problems. Species-specific identification of microsporidia has become important because of varying levels of response to albendazole, which is the only effective treatment for some kinds of intestinal microsporidiosis. Although these parasites cause up to 50% of otherwise unexplained chronic diarrhea in HIV-positive patients, the number of reported cases is still very scarce in our country when compared to the existing HIV-positive population.

METHODS

Intestinal microsporidiosis in HIV-positive patients with diarrhea was investigated using the modified trichrome staining technique. Microsporidia species identification was done by indirect immunofluorescence (IIF) and polymerase chain reaction (PCR) with specific primers.

RESULTS

Six new cases of intestinal microsporidiosis caused by Enterocytozoon bieneusi were diagnosed in Madrid (Spain). All patients were in an advanced state of the HIV infection and they presented CD4+ values equal or inferior to 100 x 10(6)/I.

CONCLUSIONS

Due to the number of cases that are accumulating, microsporidia must be included among the enteropathogens responsible for chronic diarrhea in HIV-positive individuals in Spain. The PCR technique using specific primers is a suitable determinator of the microsporidia species implicated in this intestinal pathology.

Authors+Show Affiliations

Sección de Parasitología, Facultad de Ciencias Experimentales y Técnicas, Universidad San Pablo-CEU, Madrid.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

9527369

Citation

del Aguila, C, et al. "[Species Identification of Intestinal Microsporidiosis in HIV-positive Patients Using the Polymerase Chain Reaction]." Enfermedades Infecciosas Y Microbiologia Clinica, vol. 15, no. 9, 1997, pp. 456-61.
del Aguila C, Soriano V, Navajas R, et al. [Species identification of intestinal microsporidiosis in HIV-positive patients using the polymerase chain reaction]. Enferm Infecc Microbiol Clin. 1997;15(9):456-61.
del Aguila, C., Soriano, V., Navajas, R., Subirats, M., Fenoy, S., Valencia, E., Baquero, M., & Pieniazek, N. J. (1997). [Species identification of intestinal microsporidiosis in HIV-positive patients using the polymerase chain reaction]. Enfermedades Infecciosas Y Microbiologia Clinica, 15(9), 456-61.
del Aguila C, et al. [Species Identification of Intestinal Microsporidiosis in HIV-positive Patients Using the Polymerase Chain Reaction]. Enferm Infecc Microbiol Clin. 1997;15(9):456-61. PubMed PMID: 9527369.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Species identification of intestinal microsporidiosis in HIV-positive patients using the polymerase chain reaction]. AU - del Aguila,C, AU - Soriano,V, AU - Navajas,R, AU - Subirats,M, AU - Fenoy,S, AU - Valencia,E, AU - Baquero,M, AU - Pieniazek,N J, PY - 1998/4/4/pubmed PY - 1998/4/4/medline PY - 1998/4/4/entrez SP - 456 EP - 61 JF - Enfermedades infecciosas y microbiologia clinica JO - Enferm. Infecc. Microbiol. Clin. VL - 15 IS - 9 N2 - BACKGROUND: Microsporidia are opportunistic parasites which, due to their morphologic characteristics, continue presenting diagnostic problems. Species-specific identification of microsporidia has become important because of varying levels of response to albendazole, which is the only effective treatment for some kinds of intestinal microsporidiosis. Although these parasites cause up to 50% of otherwise unexplained chronic diarrhea in HIV-positive patients, the number of reported cases is still very scarce in our country when compared to the existing HIV-positive population. METHODS: Intestinal microsporidiosis in HIV-positive patients with diarrhea was investigated using the modified trichrome staining technique. Microsporidia species identification was done by indirect immunofluorescence (IIF) and polymerase chain reaction (PCR) with specific primers. RESULTS: Six new cases of intestinal microsporidiosis caused by Enterocytozoon bieneusi were diagnosed in Madrid (Spain). All patients were in an advanced state of the HIV infection and they presented CD4+ values equal or inferior to 100 x 10(6)/I. CONCLUSIONS: Due to the number of cases that are accumulating, microsporidia must be included among the enteropathogens responsible for chronic diarrhea in HIV-positive individuals in Spain. The PCR technique using specific primers is a suitable determinator of the microsporidia species implicated in this intestinal pathology. SN - 0213-005X UR - https://www.unboundmedicine.com/medline/citation/9527369/[Species_identification_of_intestinal_microsporidiosis_in_HIV_positive_patients_using_the_polymerase_chain_reaction]_ L2 - http://www.diseaseinfosearch.org/result/4799 DB - PRIME DP - Unbound Medicine ER -