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[Cellular immunophenotypes in 97 adults with acute leukemia].
Rev Invest Clin. 1997 Nov-Dec; 49(6):457-64.RI

Abstract

OBJECTIVE

To analyze hematopoietic cell surface antigen reactivity in acute leukemia (AL) by flow cytometry and identify acute mixed-lineage leukemias (AMLL) employing the most widely accepted criteria.

MATERIAL AND METHODS

Ninety seven patients with de novo AL were studied. Cell surface antigens were investigated with monoclonal antibodies directed to: B lymphoid (CD10, CD19, CD20, CD21, CD22); T lymphoid (CD2, CD3, CD5, CD7); and myeloid (CD13, CD14, CD15, CD33, CD41) cell lineages. Maturation cell-associated antigens (CD34, HLA-DR and TdT) were also studied.

RESULTS

Twelve patients unclassified by cytomorphology could be classified by immunophenotype. Using cytomorphologic, cytochemical and immunophenotypic data, 54 cases corresponded to acute lymphoblastic leukemia (ALL) and 43 were acute myeloblastic leukemia (AML). In All there were 63% B lineage, 15% T, 7% T/B, 6% undifferentiated and 9% mixed-lineage (coexpression of two or more myeloid-associated antigens). In AML, myeloid immunophenotype was observed in 86% undifferentiated in 2%, and mixed-lineage in 12% (coexpression of two or more lymphoid-associated antigens). In addition, 26% of ALL cases and 12% of AML cases expressed a single myeloid and lymphoid antigen respectively. The most common aberrant antigens in ALL and AML were CD13 and CD7 respectively. The highest frequency of CD34 antigen expression (90%) was detected in patients with AMLL.

CONCLUSIONS

Flow cytometric immunophenotypic analysis allowed to: a) establish diagnosis in cytomorphologically unclassified cases; b) identify AMLL with a frequency similar to that reported in other series; and c) confirm the heterogeneity of AL.

Authors+Show Affiliations

Departamento de Hematología-Oncología, Laboratorio de Biología Celular, Instituto Nacional de la Nutrición Salvador Zubirán, Vasco de Quiroga, México, D.F.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

9528305

Citation

Piedras, J, et al. "[Cellular Immunophenotypes in 97 Adults With Acute Leukemia]." Revista De Investigacion Clinica; Organo Del Hospital De Enfermedades De La Nutricion, vol. 49, no. 6, 1997, pp. 457-64.
Piedras J, López-Karpovitch X, Cárdenas MR. [Cellular immunophenotypes in 97 adults with acute leukemia]. Rev Invest Clin. 1997;49(6):457-64.
Piedras, J., López-Karpovitch, X., & Cárdenas, M. R. (1997). [Cellular immunophenotypes in 97 adults with acute leukemia]. Revista De Investigacion Clinica; Organo Del Hospital De Enfermedades De La Nutricion, 49(6), 457-64.
Piedras J, López-Karpovitch X, Cárdenas MR. [Cellular Immunophenotypes in 97 Adults With Acute Leukemia]. Rev Invest Clin. 1997 Nov-Dec;49(6):457-64. PubMed PMID: 9528305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Cellular immunophenotypes in 97 adults with acute leukemia]. AU - Piedras,J, AU - López-Karpovitch,X, AU - Cárdenas,M R, PY - 1998/4/7/pubmed PY - 1998/4/7/medline PY - 1998/4/7/entrez SP - 457 EP - 64 JF - Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion JO - Rev Invest Clin VL - 49 IS - 6 N2 - OBJECTIVE: To analyze hematopoietic cell surface antigen reactivity in acute leukemia (AL) by flow cytometry and identify acute mixed-lineage leukemias (AMLL) employing the most widely accepted criteria. MATERIAL AND METHODS: Ninety seven patients with de novo AL were studied. Cell surface antigens were investigated with monoclonal antibodies directed to: B lymphoid (CD10, CD19, CD20, CD21, CD22); T lymphoid (CD2, CD3, CD5, CD7); and myeloid (CD13, CD14, CD15, CD33, CD41) cell lineages. Maturation cell-associated antigens (CD34, HLA-DR and TdT) were also studied. RESULTS: Twelve patients unclassified by cytomorphology could be classified by immunophenotype. Using cytomorphologic, cytochemical and immunophenotypic data, 54 cases corresponded to acute lymphoblastic leukemia (ALL) and 43 were acute myeloblastic leukemia (AML). In All there were 63% B lineage, 15% T, 7% T/B, 6% undifferentiated and 9% mixed-lineage (coexpression of two or more myeloid-associated antigens). In AML, myeloid immunophenotype was observed in 86% undifferentiated in 2%, and mixed-lineage in 12% (coexpression of two or more lymphoid-associated antigens). In addition, 26% of ALL cases and 12% of AML cases expressed a single myeloid and lymphoid antigen respectively. The most common aberrant antigens in ALL and AML were CD13 and CD7 respectively. The highest frequency of CD34 antigen expression (90%) was detected in patients with AMLL. CONCLUSIONS: Flow cytometric immunophenotypic analysis allowed to: a) establish diagnosis in cytomorphologically unclassified cases; b) identify AMLL with a frequency similar to that reported in other series; and c) confirm the heterogeneity of AL. SN - 0034-8376 UR - https://www.unboundmedicine.com/medline/citation/9528305/[Cellular_immunophenotypes_in_97_adults_with_acute_leukemia]_ L2 - https://antibodies.cancer.gov/detail/CPTC-HLA-DPB1-2 DB - PRIME DP - Unbound Medicine ER -