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Genetic alterations in chronic pancreatitis: evidence for early occurrence of p53 but not K-ras mutations.
Br J Surg 1998; 85(3):337-40BJ

Abstract

BACKGROUND

In patients suffering from chronic pancreatitis the risk for the development of pancreatic cancer ranges from 4 to 6 per cent. Various mutations are associated with pancreatic cancer, especially of p53 and K-ras. The incidence of these mutations in resected chronic pancreatitic tissue was investigated.

METHODS

In the present study DNA from 80 samples of tissue from patients with chronic pancreatitis was isolated and subjected to single-strand conformation polymorphism (SSCP) analysis of p53 exons 5-9 and restriction fragment length polymorphism analysis of K-ras (codon 12).

RESULTS

No mutations in the K-ras gene were detected. On SSCP analysis, eight of 80 cases of chronic pancreatitis showed alterations (two in exon 5, four in exon 6, two in exon 7). DNA sequence analysis revealed one deletion of 21 amino acids (exon 5), four polymorphisms in exon 6 with no change in the amino acid sequence, one point mutation in exon 5, and two point mutations located in the intron between exons 6 and 7.

CONCLUSION

These data show that in some cases of chronic pancreatitis mutations in the p53 gene occur without morphological evidence of pancreatic cancer.

Authors+Show Affiliations

Department of General Surgery, University of Ulm, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9529487

Citation

Gansauge, S, et al. "Genetic Alterations in Chronic Pancreatitis: Evidence for Early Occurrence of P53 but Not K-ras Mutations." The British Journal of Surgery, vol. 85, no. 3, 1998, pp. 337-40.
Gansauge S, Schmid RM, Muller J, et al. Genetic alterations in chronic pancreatitis: evidence for early occurrence of p53 but not K-ras mutations. Br J Surg. 1998;85(3):337-40.
Gansauge, S., Schmid, R. M., Muller, J., Adler, G., Mattfeldt, T., & Beger, H. G. (1998). Genetic alterations in chronic pancreatitis: evidence for early occurrence of p53 but not K-ras mutations. The British Journal of Surgery, 85(3), pp. 337-40.
Gansauge S, et al. Genetic Alterations in Chronic Pancreatitis: Evidence for Early Occurrence of P53 but Not K-ras Mutations. Br J Surg. 1998;85(3):337-40. PubMed PMID: 9529487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic alterations in chronic pancreatitis: evidence for early occurrence of p53 but not K-ras mutations. AU - Gansauge,S, AU - Schmid,R M, AU - Muller,J, AU - Adler,G, AU - Mattfeldt,T, AU - Beger,H G, PY - 1998/4/8/pubmed PY - 1998/4/8/medline PY - 1998/4/8/entrez SP - 337 EP - 40 JF - The British journal of surgery JO - Br J Surg VL - 85 IS - 3 N2 - BACKGROUND: In patients suffering from chronic pancreatitis the risk for the development of pancreatic cancer ranges from 4 to 6 per cent. Various mutations are associated with pancreatic cancer, especially of p53 and K-ras. The incidence of these mutations in resected chronic pancreatitic tissue was investigated. METHODS: In the present study DNA from 80 samples of tissue from patients with chronic pancreatitis was isolated and subjected to single-strand conformation polymorphism (SSCP) analysis of p53 exons 5-9 and restriction fragment length polymorphism analysis of K-ras (codon 12). RESULTS: No mutations in the K-ras gene were detected. On SSCP analysis, eight of 80 cases of chronic pancreatitis showed alterations (two in exon 5, four in exon 6, two in exon 7). DNA sequence analysis revealed one deletion of 21 amino acids (exon 5), four polymorphisms in exon 6 with no change in the amino acid sequence, one point mutation in exon 5, and two point mutations located in the intron between exons 6 and 7. CONCLUSION: These data show that in some cases of chronic pancreatitis mutations in the p53 gene occur without morphological evidence of pancreatic cancer. SN - 0007-1323 UR - https://www.unboundmedicine.com/medline/citation/9529487/Genetic_alterations_in_chronic_pancreatitis:_evidence_for_early_occurrence_of_p53_but_not_K_ras_mutations_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1323&date=1998&volume=85&issue=3&spage=337 DB - PRIME DP - Unbound Medicine ER -