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Discoordinate surface expression of IFN-gamma-induced HLA class II proteins in nonprofessional antigen-presenting cells with absence of DM and class II colocalization.
J Immunol. 1998 Apr 01; 160(7):3207-16.JI

Abstract

We compared HLA class II expression in a human melanoma line (a nonprofessional APC), induced by IFN-gamma or by stable transfection with CIITA, with constitutive class II expression in an EBV-transformed B lymphoblastoid cell line (a professional APC) from the same donor. IFN-gamma-induced and CIITA-transfected melanoma cells expressed DR, DP, and DQ at levels similar to those expressed by the professional APC; however, DP and DQ proteins and DM-dependent DR epitopes were delayed in appearing on the cell surface when induced by IFN-gamma. The delay in cell surface expression of some IFN-gamma-induced class II epitopes was observed even though Northern blots demonstrated class II and DM genes to be coordinately transcribed and their mRNA levels to be equivalent to that in B lymphoblastoid cells. Confocal microscopy suggests that discoordinate cell surface expression of class II results from different intracellular trafficking for IFN-gamma-induced class II proteins in the melanoma line compared with that in professional APCs. Specifically, although DR and DM proteins were present 2 days after IFN-gamma induction, colocalization of DR and DM proteins intracellularly was not apparent in cells at any time after induction. Failure of DR and DM proteins to colocalize suggests that IFN-gamma-induced cells lack an intracellular MIIC-like compartment. The absence of a compartment containing DR and DM to facilitate interaction between the two proteins may account for the delayed surface expression of class II epitopes whose formation requires both class II and DM.

Authors+Show Affiliations

Department of Pediatrics, University of Washington, Seattle 98195, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9531276

Citation

Muczynski, K A., et al. "Discoordinate Surface Expression of IFN-gamma-induced HLA Class II Proteins in Nonprofessional Antigen-presenting Cells With Absence of DM and Class II Colocalization." Journal of Immunology (Baltimore, Md. : 1950), vol. 160, no. 7, 1998, pp. 3207-16.
Muczynski KA, Anderson SK, Pious D. Discoordinate surface expression of IFN-gamma-induced HLA class II proteins in nonprofessional antigen-presenting cells with absence of DM and class II colocalization. J Immunol. 1998;160(7):3207-16.
Muczynski, K. A., Anderson, S. K., & Pious, D. (1998). Discoordinate surface expression of IFN-gamma-induced HLA class II proteins in nonprofessional antigen-presenting cells with absence of DM and class II colocalization. Journal of Immunology (Baltimore, Md. : 1950), 160(7), 3207-16.
Muczynski KA, Anderson SK, Pious D. Discoordinate Surface Expression of IFN-gamma-induced HLA Class II Proteins in Nonprofessional Antigen-presenting Cells With Absence of DM and Class II Colocalization. J Immunol. 1998 Apr 1;160(7):3207-16. PubMed PMID: 9531276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discoordinate surface expression of IFN-gamma-induced HLA class II proteins in nonprofessional antigen-presenting cells with absence of DM and class II colocalization. AU - Muczynski,K A, AU - Anderson,S K, AU - Pious,D, PY - 1998/4/8/pubmed PY - 1998/4/8/medline PY - 1998/4/8/entrez SP - 3207 EP - 16 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 160 IS - 7 N2 - We compared HLA class II expression in a human melanoma line (a nonprofessional APC), induced by IFN-gamma or by stable transfection with CIITA, with constitutive class II expression in an EBV-transformed B lymphoblastoid cell line (a professional APC) from the same donor. IFN-gamma-induced and CIITA-transfected melanoma cells expressed DR, DP, and DQ at levels similar to those expressed by the professional APC; however, DP and DQ proteins and DM-dependent DR epitopes were delayed in appearing on the cell surface when induced by IFN-gamma. The delay in cell surface expression of some IFN-gamma-induced class II epitopes was observed even though Northern blots demonstrated class II and DM genes to be coordinately transcribed and their mRNA levels to be equivalent to that in B lymphoblastoid cells. Confocal microscopy suggests that discoordinate cell surface expression of class II results from different intracellular trafficking for IFN-gamma-induced class II proteins in the melanoma line compared with that in professional APCs. Specifically, although DR and DM proteins were present 2 days after IFN-gamma induction, colocalization of DR and DM proteins intracellularly was not apparent in cells at any time after induction. Failure of DR and DM proteins to colocalize suggests that IFN-gamma-induced cells lack an intracellular MIIC-like compartment. The absence of a compartment containing DR and DM to facilitate interaction between the two proteins may account for the delayed surface expression of class II epitopes whose formation requires both class II and DM. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9531276/Discoordinate_surface_expression_of_IFN_gamma_induced_HLA_class_II_proteins_in_nonprofessional_antigen_presenting_cells_with_absence_of_DM_and_class_II_colocalization_ DB - PRIME DP - Unbound Medicine ER -