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Rat testicular germ cells and epididymal sperm contain active P450 aromatase.
J Androl. 1998 Jan-Feb; 19(1):65-71.JA

Abstract

Although testosterone is the principal sex steroid produced by the testis, estrogen is known to be produced by both Leydig and Sertoli cells during different developmental periods. Additionally, evidence is unfolding to suggest that germ cells might also participate in the synthesis of estrogen within the male reproductive tract. We have recently reported that the messenger ribonucleic acid (mRNA) for P450 aromatase (P450arom), the enzyme that converts androgen to estrogen, is synthesized by rat germ cells. Therefore, the present study was conducted to determine which germ cell types synthesize active P450arom and to measure the activity of this enzyme in germ cells throughout spermatogenesis and in maturing sperm during epididymal transit. First, P450arom activity was measured in pachytene spermatocytes, round spermatids, and a mixture of round spermatids, elongating spermatids, and residual bodies using the tritiated water (3H2O) assay. Second, sperm isolated from different regions of the epididymis were assayed for P450arom activity. Sperm isolated from the caput epididymis with attached efferent ductules had the higher P450arom activity, whereas sperm isolated from the corpus and cauda epididymides had lower P450arom activity. The decrease in P450arom activity in cauda sperm was further confirmed by immunocytochemistry. On the basis of these observations, we conclude that rat testicular germ cells from pachytene spermatocytes through elongating spermatids and epididymal sperm contain active P450arom and that sperm lose aromatase activity as they mature during epididymal transit. Therefore, both post-pachytene rat germ cells and epididymal sperm are capable of estrogen synthesis and are an additional, potentially significant, source of estrogen in the male reproductive tract.

Authors+Show Affiliations

Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana 61802, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9537293

Citation

Janulis, L, et al. "Rat Testicular Germ Cells and Epididymal Sperm Contain Active P450 Aromatase." Journal of Andrology, vol. 19, no. 1, 1998, pp. 65-71.
Janulis L, Bahr JM, Hess RA, et al. Rat testicular germ cells and epididymal sperm contain active P450 aromatase. J Androl. 1998;19(1):65-71.
Janulis, L., Bahr, J. M., Hess, R. A., Janssen, S., Osawa, Y., & Bunick, D. (1998). Rat testicular germ cells and epididymal sperm contain active P450 aromatase. Journal of Andrology, 19(1), 65-71.
Janulis L, et al. Rat Testicular Germ Cells and Epididymal Sperm Contain Active P450 Aromatase. J Androl. 1998 Jan-Feb;19(1):65-71. PubMed PMID: 9537293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rat testicular germ cells and epididymal sperm contain active P450 aromatase. AU - Janulis,L, AU - Bahr,J M, AU - Hess,R A, AU - Janssen,S, AU - Osawa,Y, AU - Bunick,D, PY - 1998/4/16/pubmed PY - 1998/4/16/medline PY - 1998/4/16/entrez SP - 65 EP - 71 JF - Journal of andrology JO - J Androl VL - 19 IS - 1 N2 - Although testosterone is the principal sex steroid produced by the testis, estrogen is known to be produced by both Leydig and Sertoli cells during different developmental periods. Additionally, evidence is unfolding to suggest that germ cells might also participate in the synthesis of estrogen within the male reproductive tract. We have recently reported that the messenger ribonucleic acid (mRNA) for P450 aromatase (P450arom), the enzyme that converts androgen to estrogen, is synthesized by rat germ cells. Therefore, the present study was conducted to determine which germ cell types synthesize active P450arom and to measure the activity of this enzyme in germ cells throughout spermatogenesis and in maturing sperm during epididymal transit. First, P450arom activity was measured in pachytene spermatocytes, round spermatids, and a mixture of round spermatids, elongating spermatids, and residual bodies using the tritiated water (3H2O) assay. Second, sperm isolated from different regions of the epididymis were assayed for P450arom activity. Sperm isolated from the caput epididymis with attached efferent ductules had the higher P450arom activity, whereas sperm isolated from the corpus and cauda epididymides had lower P450arom activity. The decrease in P450arom activity in cauda sperm was further confirmed by immunocytochemistry. On the basis of these observations, we conclude that rat testicular germ cells from pachytene spermatocytes through elongating spermatids and epididymal sperm contain active P450arom and that sperm lose aromatase activity as they mature during epididymal transit. Therefore, both post-pachytene rat germ cells and epididymal sperm are capable of estrogen synthesis and are an additional, potentially significant, source of estrogen in the male reproductive tract. SN - 0196-3635 UR - https://www.unboundmedicine.com/medline/citation/9537293/Rat_testicular_germ_cells_and_epididymal_sperm_contain_active_P450_aromatase_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0196-3635&date=1998&volume=19&issue=1&spage=65 DB - PRIME DP - Unbound Medicine ER -