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Estrogen-induced keratinocyte growth factor mRNA expression in normal and cancerous human breast cells.
Oncol Rep. 1998 May-Jun; 5(3):577-83.OR

Abstract

The local recurrence rate of breast cancer has been reported to be unusually high at the surgical scar. Such breast cancer recurrence is believed to be triggered by the release of growth factors into the healing wound. Observations from an animal model have also demonstrated that KGF expression is dramatically induced by creation of full thickness wounds in mouse skin. Since KGF is an epithelial cell-specific mitogen in rat mammary epithelium, it is reasonable to speculate that KGF may be also involved in regulating human breast cancer cell growth. The purpose of the present study was to determine the effect of estradiol-17 on KGF gene expression in normal human breast stromal cells, as well as in human breast cancer stromal cells, and the mechanisms by which estradiol-17 regulates breast epithelial proliferation. Our results show that KGF expression was not effected by estradiol-17 treatment in normal human breast stromal cells. In contrast, KGF expression was stimulated by estradiol-17 in human breast cancer stromal cells. KGF mRNA levels have also been examined in normal human breast stromal cells and human breast cancer stromal cells. An interesting correlation was found between KGF expression and estradiol-17 regulation in these cell types. Normal human breast stromal cells which do not response to estradiol-17 have lower KGF mRNA level than the cancer cells which KGF expression is stimulated by estradiol-17. Our data also demonstrate that recombinant human KGF significantly stimulate normal human breast and human breast cancer epithelial cell proliferation in a dose-dependent manner. Since we have shown that estradiol-17 induces KGF mRNA expression in human breast cancer stromal cells, KGF may be involved at least in part in the stimulatory pathway that is initiated by estradiol-17 in human breast cancer epithelial cells.

Authors+Show Affiliations

Laboratory of Reproductive and Molecular Endocrinology, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9538155

Citation

Zhang, Y, et al. "Estrogen-induced Keratinocyte Growth Factor mRNA Expression in Normal and Cancerous Human Breast Cells." Oncology Reports, vol. 5, no. 3, 1998, pp. 577-83.
Zhang Y, Sugimoto Y, Kulp SK, et al. Estrogen-induced keratinocyte growth factor mRNA expression in normal and cancerous human breast cells. Oncol Rep. 1998;5(3):577-83.
Zhang, Y., Sugimoto, Y., Kulp, S. K., Farrar, W. B., Brueggemeier, R. W., & Lin, Y. C. (1998). Estrogen-induced keratinocyte growth factor mRNA expression in normal and cancerous human breast cells. Oncology Reports, 5(3), 577-83.
Zhang Y, et al. Estrogen-induced Keratinocyte Growth Factor mRNA Expression in Normal and Cancerous Human Breast Cells. Oncol Rep. 1998 May-Jun;5(3):577-83. PubMed PMID: 9538155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estrogen-induced keratinocyte growth factor mRNA expression in normal and cancerous human breast cells. AU - Zhang,Y, AU - Sugimoto,Y, AU - Kulp,S K, AU - Farrar,W B, AU - Brueggemeier,R W, AU - Lin,Y C, PY - 1998/5/9/pubmed PY - 1998/5/9/medline PY - 1998/5/9/entrez SP - 577 EP - 83 JF - Oncology reports JO - Oncol Rep VL - 5 IS - 3 N2 - The local recurrence rate of breast cancer has been reported to be unusually high at the surgical scar. Such breast cancer recurrence is believed to be triggered by the release of growth factors into the healing wound. Observations from an animal model have also demonstrated that KGF expression is dramatically induced by creation of full thickness wounds in mouse skin. Since KGF is an epithelial cell-specific mitogen in rat mammary epithelium, it is reasonable to speculate that KGF may be also involved in regulating human breast cancer cell growth. The purpose of the present study was to determine the effect of estradiol-17 on KGF gene expression in normal human breast stromal cells, as well as in human breast cancer stromal cells, and the mechanisms by which estradiol-17 regulates breast epithelial proliferation. Our results show that KGF expression was not effected by estradiol-17 treatment in normal human breast stromal cells. In contrast, KGF expression was stimulated by estradiol-17 in human breast cancer stromal cells. KGF mRNA levels have also been examined in normal human breast stromal cells and human breast cancer stromal cells. An interesting correlation was found between KGF expression and estradiol-17 regulation in these cell types. Normal human breast stromal cells which do not response to estradiol-17 have lower KGF mRNA level than the cancer cells which KGF expression is stimulated by estradiol-17. Our data also demonstrate that recombinant human KGF significantly stimulate normal human breast and human breast cancer epithelial cell proliferation in a dose-dependent manner. Since we have shown that estradiol-17 induces KGF mRNA expression in human breast cancer stromal cells, KGF may be involved at least in part in the stimulatory pathway that is initiated by estradiol-17 in human breast cancer epithelial cells. SN - 1021-335X UR - https://www.unboundmedicine.com/medline/citation/9538155/Estrogen_induced_keratinocyte_growth_factor_mRNA_expression_in_normal_and_cancerous_human_breast_cells_ L2 - http://www.spandidos-publications.com/or/5/3/577 DB - PRIME DP - Unbound Medicine ER -