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Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings.
J Biochem. 1998 Mar; 123(3):386-91.JB

Abstract

Oleosins are hydrophobic proteins localized abundantly in the oil bodies of plant seeds. Two distinct oleosin isoforms of molecular masses 18 and 16 kDa are present in rice oil bodies. These isoforms were found in similar ratio in rice embryos and aleurone layers. To survey potential DNA sequences involved in the activation of oleosin genes, a genomic clone of rice 18 kDa oleosin was sequenced, and its 5'-flanking region was compared with that of the known rice 16 kDa oleosin gene. Corresponding mRNAs of the two rice oleosin isoforms appeared seven days after pollination and vanished in mature seeds. Triacylglycerols and oleosins were accumulated concomitantly in maturing rice reeds in accord with the active assembly of oil bodies, and partly mobilized in postgerminative seedlings. Approximately 60% of the stored triacylglycerols in rice were not utilized: while the majority of oil bodies in embryos were mobilized in five days after imbibition, those in aleurone layers remained intact in postgerminative seedlings.

Authors+Show Affiliations

Graduate Institute of Agricultural Biotechnology National Chung-Hsing University, Taichung, Taiwan 40227.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9538219

Citation

Wu, L S., et al. "Genomic Cloning of 18 kDa Oleosin and Detection of Triacylglycerols and Oleosin Isoforms in Maturing Rice and Postgerminative Seedlings." Journal of Biochemistry, vol. 123, no. 3, 1998, pp. 386-91.
Wu LS, Wang LD, Chen PW, et al. Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings. J Biochem. 1998;123(3):386-91.
Wu, L. S., Wang, L. D., Chen, P. W., Chen, L. J., & Tzen, J. T. (1998). Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings. Journal of Biochemistry, 123(3), 386-91.
Wu LS, et al. Genomic Cloning of 18 kDa Oleosin and Detection of Triacylglycerols and Oleosin Isoforms in Maturing Rice and Postgerminative Seedlings. J Biochem. 1998;123(3):386-91. PubMed PMID: 9538219.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genomic cloning of 18 kDa oleosin and detection of triacylglycerols and oleosin isoforms in maturing rice and postgerminative seedlings. AU - Wu,L S, AU - Wang,L D, AU - Chen,P W, AU - Chen,L J, AU - Tzen,J T, PY - 1998/5/30/pubmed PY - 1998/5/30/medline PY - 1998/5/30/entrez SP - 386 EP - 91 JF - Journal of biochemistry JO - J Biochem VL - 123 IS - 3 N2 - Oleosins are hydrophobic proteins localized abundantly in the oil bodies of plant seeds. Two distinct oleosin isoforms of molecular masses 18 and 16 kDa are present in rice oil bodies. These isoforms were found in similar ratio in rice embryos and aleurone layers. To survey potential DNA sequences involved in the activation of oleosin genes, a genomic clone of rice 18 kDa oleosin was sequenced, and its 5'-flanking region was compared with that of the known rice 16 kDa oleosin gene. Corresponding mRNAs of the two rice oleosin isoforms appeared seven days after pollination and vanished in mature seeds. Triacylglycerols and oleosins were accumulated concomitantly in maturing rice reeds in accord with the active assembly of oil bodies, and partly mobilized in postgerminative seedlings. Approximately 60% of the stored triacylglycerols in rice were not utilized: while the majority of oil bodies in embryos were mobilized in five days after imbibition, those in aleurone layers remained intact in postgerminative seedlings. SN - 0021-924X UR - https://www.unboundmedicine.com/medline/citation/9538219/Genomic_cloning_of_18_kDa_oleosin_and_detection_of_triacylglycerols_and_oleosin_isoforms_in_maturing_rice_and_postgerminative_seedlings_ L2 - https://joi.jlc.jst.go.jp/JST.Journalarchive/biochemistry1922/123.386?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -