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Absorption-enhancing effects of sodium caprate and palmitoyl carnitine in rat and human colons.
Dig Dis Sci. 1998 Mar; 43(3):641-5.DD

Abstract

We examined the enhancing action of sodium caprate and palmitoylcarnitine on the permeability of fluorescein isothiocyanate dextran 4000 as a paracellular permeant compound in isolated rat and human colon samples using the Ussing-type chamber method. In the absence of an enhancer, the permeation clearance of fluorescein isothiocyanate dextran 4000 was not significantly different in the rat and human colons, but the electric membrane resistance was smaller in the rat colon than in the human colon. Sodium caprate and palmitoylcarnitine increased permeation clearance and decreased electric membrane resistance in both types of colonic membrane, showing that the rat colon can be used as a model of the human colon for studies of enhancer effects. A calmodulin antagonist significantly inhibited the action of sodium caprate in both colonic membranes. However, it tended to promote the effects of palmitoylcarnitine on permeation clearance and electric membrane resistance. These results suggest that sodium caprate induces the contraction of the perijunctional actomyosin ring to widen the tight junction and that the mechanism of palmitoylcarnitine is different from that of sodium caprate in the human colon, as reported previously for Caco-2 cell monolayers.

Authors+Show Affiliations

Department of Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9539662

Citation

Shimazaki, T, et al. "Absorption-enhancing Effects of Sodium Caprate and Palmitoyl Carnitine in Rat and Human Colons." Digestive Diseases and Sciences, vol. 43, no. 3, 1998, pp. 641-5.
Shimazaki T, Tomita M, Sadahiro S, et al. Absorption-enhancing effects of sodium caprate and palmitoyl carnitine in rat and human colons. Dig Dis Sci. 1998;43(3):641-5.
Shimazaki, T., Tomita, M., Sadahiro, S., Hayashi, M., & Awazu, S. (1998). Absorption-enhancing effects of sodium caprate and palmitoyl carnitine in rat and human colons. Digestive Diseases and Sciences, 43(3), 641-5.
Shimazaki T, et al. Absorption-enhancing Effects of Sodium Caprate and Palmitoyl Carnitine in Rat and Human Colons. Dig Dis Sci. 1998;43(3):641-5. PubMed PMID: 9539662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absorption-enhancing effects of sodium caprate and palmitoyl carnitine in rat and human colons. AU - Shimazaki,T, AU - Tomita,M, AU - Sadahiro,S, AU - Hayashi,M, AU - Awazu,S, PY - 1998/4/16/pubmed PY - 1998/4/16/medline PY - 1998/4/16/entrez SP - 641 EP - 5 JF - Digestive diseases and sciences JO - Dig Dis Sci VL - 43 IS - 3 N2 - We examined the enhancing action of sodium caprate and palmitoylcarnitine on the permeability of fluorescein isothiocyanate dextran 4000 as a paracellular permeant compound in isolated rat and human colon samples using the Ussing-type chamber method. In the absence of an enhancer, the permeation clearance of fluorescein isothiocyanate dextran 4000 was not significantly different in the rat and human colons, but the electric membrane resistance was smaller in the rat colon than in the human colon. Sodium caprate and palmitoylcarnitine increased permeation clearance and decreased electric membrane resistance in both types of colonic membrane, showing that the rat colon can be used as a model of the human colon for studies of enhancer effects. A calmodulin antagonist significantly inhibited the action of sodium caprate in both colonic membranes. However, it tended to promote the effects of palmitoylcarnitine on permeation clearance and electric membrane resistance. These results suggest that sodium caprate induces the contraction of the perijunctional actomyosin ring to widen the tight junction and that the mechanism of palmitoylcarnitine is different from that of sodium caprate in the human colon, as reported previously for Caco-2 cell monolayers. SN - 0163-2116 UR - https://www.unboundmedicine.com/medline/citation/9539662/Absorption_enhancing_effects_of_sodium_caprate_and_palmitoyl_carnitine_in_rat_and_human_colons_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=9539662.ui DB - PRIME DP - Unbound Medicine ER -