Tags

Type your tag names separated by a space and hit enter

Refined mapping of the human dentin sialophosphoprotein (DSPP) gene within the critical dentinogenesis imperfecta type II and dentin dysplasia type II loci.
Eur J Oral Sci. 1998 Jan; 106 Suppl 1:227-33.EJ

Abstract

Dentinogenesis imperfecta type II and dentin dysplasia type II are diseases resulting in abnormal dentin formation, which have been mapped to overlapping regions of human chromosome 4q defined by markers D4S2691 and D4S2692 (6.6 cM) and D4S3291 and SPP1 (14.1 cM), respectively. Recently, two of the major non-collagenous proteins of dentin, dentin sialoprotein (DSP) and dentin phosphoprotein (DPP, phosphophoryn) have been shown to be encoded by a single gene, termed dentin sialophosphoprotein (DSPP), which has been mapped to human chromosome 4. The purpose of this study was to perform refined mapping of DSPP related to these disease loci by gene content mapping, as well as to place the DSPP gene on the physical map of human chromosome 4 by sequence tagged site (STS) content mapping. Human genomic DSPP clones were isolated, and gene content mapping performed with specific primers for dentin matrix protein 1 (DMP1), bone sialoprotein (BSP) and osteopontin (secreted phosphoprotein 1, SPP1). STS content mapping was then performed with flanking STS markers to these dentin/bone gene loci. Our results demonstrate that the DSPP and DMP1 genes are within a maximum distance of 110 kb. Both DSPP and DMP-1 have been placed on the physical map of human chromosome 4 within the interval defined by markers D4S564 and D4S1292. DSPP is thereby strengthened as a candidate gene for both DGI-II and DD-II.

Authors+Show Affiliations

Department of Pediatric Dentistry, University of Texas Health Science Center at San Antonio, 78284-8888, USA. macdougall@uthscsa.edu

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9541230

Citation

MacDougall, M. "Refined Mapping of the Human Dentin Sialophosphoprotein (DSPP) Gene Within the Critical Dentinogenesis Imperfecta Type II and Dentin Dysplasia Type II Loci." European Journal of Oral Sciences, vol. 106 Suppl 1, 1998, pp. 227-33.
MacDougall M. Refined mapping of the human dentin sialophosphoprotein (DSPP) gene within the critical dentinogenesis imperfecta type II and dentin dysplasia type II loci. Eur J Oral Sci. 1998;106 Suppl 1:227-33.
MacDougall, M. (1998). Refined mapping of the human dentin sialophosphoprotein (DSPP) gene within the critical dentinogenesis imperfecta type II and dentin dysplasia type II loci. European Journal of Oral Sciences, 106 Suppl 1, 227-33.
MacDougall M. Refined Mapping of the Human Dentin Sialophosphoprotein (DSPP) Gene Within the Critical Dentinogenesis Imperfecta Type II and Dentin Dysplasia Type II Loci. Eur J Oral Sci. 1998;106 Suppl 1:227-33. PubMed PMID: 9541230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Refined mapping of the human dentin sialophosphoprotein (DSPP) gene within the critical dentinogenesis imperfecta type II and dentin dysplasia type II loci. A1 - MacDougall,M, PY - 1998/4/16/pubmed PY - 1998/4/16/medline PY - 1998/4/16/entrez SP - 227 EP - 33 JF - European journal of oral sciences JO - Eur J Oral Sci VL - 106 Suppl 1 N2 - Dentinogenesis imperfecta type II and dentin dysplasia type II are diseases resulting in abnormal dentin formation, which have been mapped to overlapping regions of human chromosome 4q defined by markers D4S2691 and D4S2692 (6.6 cM) and D4S3291 and SPP1 (14.1 cM), respectively. Recently, two of the major non-collagenous proteins of dentin, dentin sialoprotein (DSP) and dentin phosphoprotein (DPP, phosphophoryn) have been shown to be encoded by a single gene, termed dentin sialophosphoprotein (DSPP), which has been mapped to human chromosome 4. The purpose of this study was to perform refined mapping of DSPP related to these disease loci by gene content mapping, as well as to place the DSPP gene on the physical map of human chromosome 4 by sequence tagged site (STS) content mapping. Human genomic DSPP clones were isolated, and gene content mapping performed with specific primers for dentin matrix protein 1 (DMP1), bone sialoprotein (BSP) and osteopontin (secreted phosphoprotein 1, SPP1). STS content mapping was then performed with flanking STS markers to these dentin/bone gene loci. Our results demonstrate that the DSPP and DMP1 genes are within a maximum distance of 110 kb. Both DSPP and DMP-1 have been placed on the physical map of human chromosome 4 within the interval defined by markers D4S564 and D4S1292. DSPP is thereby strengthened as a candidate gene for both DGI-II and DD-II. SN - 0909-8836 UR - https://www.unboundmedicine.com/medline/citation/9541230/Refined_mapping_of_the_human_dentin_sialophosphoprotein__DSPP__gene_within_the_critical_dentinogenesis_imperfecta_type_II_and_dentin_dysplasia_type_II_loci_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0909-8836&date=1998&volume=106&issue=&spage=227 DB - PRIME DP - Unbound Medicine ER -