Tags

Type your tag names separated by a space and hit enter

Expression and immunolocalization of functional cytochrome P450 aromatase in mature rat testicular cells.
Biol Reprod. 1998 Apr; 58(4):919-26.BR

Abstract

Aromatase activity has been measured in Leydig cells and Sertoli cells from both immature and mature rats. Cytochrome P450 aromatase (P450arom) has been immunolocalized in germ cells of the rodent, bear, and rooster. Our purpose was to investigate expression of and to immunolocalize P450arom in adult rat testicular cells. After Western blotting with a specific anti-cytochrome P450arom antibody, we demonstrated the presence of a 55-kDa protein in mature rat seminiferous tubules and crude germ cell preparations. Immunoreactive aromatase was detected both in cultured rat Leydig cells and in testis sections (interstitial tissue and elongated spermatids showed positive immunoreactivity for P450arom). We next used reverse transcription-polymerase chain reaction to localize and quantify the P450arom mRNA in the various testicular cells. In rat Leydig cells, the amount of P450arom mRNA was 15 times higher than in Sertoli cells (34.1+/-3.2 to 2.3 +/-0.2 x 10(-3) amol/10(6) cells, respectively). In pachytene spermatocytes, round spermatids, and testicular spermatozoa the P450arom mRNA levels were 38.7+/-8.1, 20.4+/-3.8, and < 1.3 x 10(-3) amol/10(6) cells, respectively. The aromatase activity was 2.5-4 times higher in testicular spermatozoa (8.48+/-1.98 fmol/10(6) cells per hour) than in other germ cells. These results indicate that in mature rats, not only Leydig cells and Sertoli cells but also germ cells have the capacity to express functional P450arom. According to the germ cell maturation state, there was an inverse relationship between P450arom mRNA content and the biological activity of the protein. The expression of the functional P450arom in mature rat germ cells confirms the existence of an additional source of estrogens within the genital tract of the male.

Authors+Show Affiliations

Department of Biochemistry, University of Caen, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9546721

Citation

Levallet, J, et al. "Expression and Immunolocalization of Functional Cytochrome P450 Aromatase in Mature Rat Testicular Cells." Biology of Reproduction, vol. 58, no. 4, 1998, pp. 919-26.
Levallet J, Bilinska B, Mittre H, et al. Expression and immunolocalization of functional cytochrome P450 aromatase in mature rat testicular cells. Biol Reprod. 1998;58(4):919-26.
Levallet, J., Bilinska, B., Mittre, H., Genissel, C., Fresnel, J., & Carreau, S. (1998). Expression and immunolocalization of functional cytochrome P450 aromatase in mature rat testicular cells. Biology of Reproduction, 58(4), 919-26.
Levallet J, et al. Expression and Immunolocalization of Functional Cytochrome P450 Aromatase in Mature Rat Testicular Cells. Biol Reprod. 1998;58(4):919-26. PubMed PMID: 9546721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and immunolocalization of functional cytochrome P450 aromatase in mature rat testicular cells. AU - Levallet,J, AU - Bilinska,B, AU - Mittre,H, AU - Genissel,C, AU - Fresnel,J, AU - Carreau,S, PY - 1998/4/18/pubmed PY - 1998/4/18/medline PY - 1998/4/18/entrez SP - 919 EP - 26 JF - Biology of reproduction JO - Biol Reprod VL - 58 IS - 4 N2 - Aromatase activity has been measured in Leydig cells and Sertoli cells from both immature and mature rats. Cytochrome P450 aromatase (P450arom) has been immunolocalized in germ cells of the rodent, bear, and rooster. Our purpose was to investigate expression of and to immunolocalize P450arom in adult rat testicular cells. After Western blotting with a specific anti-cytochrome P450arom antibody, we demonstrated the presence of a 55-kDa protein in mature rat seminiferous tubules and crude germ cell preparations. Immunoreactive aromatase was detected both in cultured rat Leydig cells and in testis sections (interstitial tissue and elongated spermatids showed positive immunoreactivity for P450arom). We next used reverse transcription-polymerase chain reaction to localize and quantify the P450arom mRNA in the various testicular cells. In rat Leydig cells, the amount of P450arom mRNA was 15 times higher than in Sertoli cells (34.1+/-3.2 to 2.3 +/-0.2 x 10(-3) amol/10(6) cells, respectively). In pachytene spermatocytes, round spermatids, and testicular spermatozoa the P450arom mRNA levels were 38.7+/-8.1, 20.4+/-3.8, and < 1.3 x 10(-3) amol/10(6) cells, respectively. The aromatase activity was 2.5-4 times higher in testicular spermatozoa (8.48+/-1.98 fmol/10(6) cells per hour) than in other germ cells. These results indicate that in mature rats, not only Leydig cells and Sertoli cells but also germ cells have the capacity to express functional P450arom. According to the germ cell maturation state, there was an inverse relationship between P450arom mRNA content and the biological activity of the protein. The expression of the functional P450arom in mature rat germ cells confirms the existence of an additional source of estrogens within the genital tract of the male. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/9546721/Expression_and_immunolocalization_of_functional_cytochrome_P450_aromatase_in_mature_rat_testicular_cells_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod58.4.919 DB - PRIME DP - Unbound Medicine ER -