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Hydroxyl radical activity in thermo-catalytically bleached root-filled teeth.
Endod Dent Traumatol. 1997 Jun; 13(3):119-25.ED

Abstract

Intra-coronal bleaching of root-filled teeth has been associated with invasive cervical root resorption. It is considered that during bleaching hydrogen peroxide diffuses through the tooth structure into the cervical periodontium, resulting in periodontal tissue destruction and initiating a resorptive process. Hydrogen peroxide is capable of generating hydroxyl radical, an oxygen-derived free radical, in the presence of ferrous salts. Hydroxyl radicals are extremely reactive and have been shown to degrade components of connective tissue, particularly collagen and hyaluronic acid. The aim of the present study was to determine whether hydroxyl radicals are generated during the bleaching of root-filled teeth which have been discoloured by blood. Forty extracted human premolar teeth were root-filled with gutta-percha and AH26 sealer cement. Twenty of the teeth were experimentally discoloured by blood. All teeth were then thermo-catalytically bleached using 30% hydrogen peroxide while tooth roots were seated in a test solution of sodium salicylate. Hydroxyl radical generation was determined by the detection of reaction products of this radical with salicylate using high performance liquid chromatography with electrochemical detection (HPLC-ECD). The presence of hydroxyl radicals was detected in twenty-five of the teeth. There was a significant association between the production of hydroxyl radicals and the presence of tooth discolouration caused by blood components. Greatest yields of hydroxyl radicals occurred in teeth in which EDTA had been used to clean the pulp chamber prior to bleaching. It was concluded that hydroxyl radicals are generated during the thermo-catalytic bleaching of root-filled teeth. Generation of this toxic chemical species may be one mechanism underlying periodontal tissue destruction and root resorption after intra-coronal bleaching.

Authors+Show Affiliations

Department of Dentistry, University of Adelaide, South Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9550025

Citation

Dahlstrom, S W., et al. "Hydroxyl Radical Activity in Thermo-catalytically Bleached Root-filled Teeth." Endodontics & Dental Traumatology, vol. 13, no. 3, 1997, pp. 119-25.
Dahlstrom SW, Heithersay GS, Bridges TE. Hydroxyl radical activity in thermo-catalytically bleached root-filled teeth. Endod Dent Traumatol. 1997;13(3):119-25.
Dahlstrom, S. W., Heithersay, G. S., & Bridges, T. E. (1997). Hydroxyl radical activity in thermo-catalytically bleached root-filled teeth. Endodontics & Dental Traumatology, 13(3), 119-25.
Dahlstrom SW, Heithersay GS, Bridges TE. Hydroxyl Radical Activity in Thermo-catalytically Bleached Root-filled Teeth. Endod Dent Traumatol. 1997;13(3):119-25. PubMed PMID: 9550025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydroxyl radical activity in thermo-catalytically bleached root-filled teeth. AU - Dahlstrom,S W, AU - Heithersay,G S, AU - Bridges,T E, PY - 1997/6/1/pubmed PY - 1998/4/29/medline PY - 1997/6/1/entrez SP - 119 EP - 25 JF - Endodontics & dental traumatology JO - Endod Dent Traumatol VL - 13 IS - 3 N2 - Intra-coronal bleaching of root-filled teeth has been associated with invasive cervical root resorption. It is considered that during bleaching hydrogen peroxide diffuses through the tooth structure into the cervical periodontium, resulting in periodontal tissue destruction and initiating a resorptive process. Hydrogen peroxide is capable of generating hydroxyl radical, an oxygen-derived free radical, in the presence of ferrous salts. Hydroxyl radicals are extremely reactive and have been shown to degrade components of connective tissue, particularly collagen and hyaluronic acid. The aim of the present study was to determine whether hydroxyl radicals are generated during the bleaching of root-filled teeth which have been discoloured by blood. Forty extracted human premolar teeth were root-filled with gutta-percha and AH26 sealer cement. Twenty of the teeth were experimentally discoloured by blood. All teeth were then thermo-catalytically bleached using 30% hydrogen peroxide while tooth roots were seated in a test solution of sodium salicylate. Hydroxyl radical generation was determined by the detection of reaction products of this radical with salicylate using high performance liquid chromatography with electrochemical detection (HPLC-ECD). The presence of hydroxyl radicals was detected in twenty-five of the teeth. There was a significant association between the production of hydroxyl radicals and the presence of tooth discolouration caused by blood components. Greatest yields of hydroxyl radicals occurred in teeth in which EDTA had been used to clean the pulp chamber prior to bleaching. It was concluded that hydroxyl radicals are generated during the thermo-catalytic bleaching of root-filled teeth. Generation of this toxic chemical species may be one mechanism underlying periodontal tissue destruction and root resorption after intra-coronal bleaching. SN - 0109-2502 UR - https://www.unboundmedicine.com/medline/citation/9550025/Hydroxyl_radical_activity_in_thermo_catalytically_bleached_root_filled_teeth_ DB - PRIME DP - Unbound Medicine ER -