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Independent regulation of cutaneous lymphocyte-associated antigen expression and cytokine synthesis phenotype during human CD4+ memory T cell differentiation.
J Immunol. 1997 Dec 15; 159(12):6018-29.JI

Abstract

Although considerable attention has been paid to the development of cytokine synthesis heterogeneity during memory T cell differentiation, little information is available on how this function is coregulated with homing receptor expression. The development of skin-homing, CD4+ memory T cells in the human provides an excellent model for such investigation, since 1) the skin supports both Th1- and Th2-predominant responses in different settings, and 2) the skin-homing capability of human memory T cells correlates with and appears to depend on expression of the skin-selective homing receptor cutaneous lymphocyte-associated Ag (CLA). In this study, we used multiparameter FACS analysis to examine expression of CLA vs IFN-gamma, IL-4, and IL-2 synthesis capabilities among fresh peripheral blood CD4+ memory T cells, and Th1 vs Th2 memory T cells generated in vitro from purified CD4+ naive precursors by cyclic activation in polarizing culture conditions. Among normal peripheral blood T cells, CLA expression was essentially identical among the IFN-gamma- vs IL-4-producing CD4+ memory subsets, clearly indicating the existence of in vivo mechanisms capable of producing both Th1 vs Th2 skin-homing T cells. In vitro differentiation of naive CD4+ T cells confirmed the independent regulation of CLA and all three cytokines examined, regulation that allowed differential production of IFN-gamma-, IL-4-, and IL-2-producing, CLA+ memory subsets. These studies also 1) demonstrated differences in regulatory factor activity depending on the differentiation status of the responding cell, and 2) revealed CLA expression to be much more rapidly reversible on established memory cells than cytokine synthesis capabilities.

Authors+Show Affiliations

Department of Pathology, The University of Texas Southwestern Medical Center, Dallas 75235, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9550400

Citation

Teraki, Y, and L J. Picker. "Independent Regulation of Cutaneous Lymphocyte-associated Antigen Expression and Cytokine Synthesis Phenotype During Human CD4+ Memory T Cell Differentiation." Journal of Immunology (Baltimore, Md. : 1950), vol. 159, no. 12, 1997, pp. 6018-29.
Teraki Y, Picker LJ. Independent regulation of cutaneous lymphocyte-associated antigen expression and cytokine synthesis phenotype during human CD4+ memory T cell differentiation. J Immunol. 1997;159(12):6018-29.
Teraki, Y., & Picker, L. J. (1997). Independent regulation of cutaneous lymphocyte-associated antigen expression and cytokine synthesis phenotype during human CD4+ memory T cell differentiation. Journal of Immunology (Baltimore, Md. : 1950), 159(12), 6018-29.
Teraki Y, Picker LJ. Independent Regulation of Cutaneous Lymphocyte-associated Antigen Expression and Cytokine Synthesis Phenotype During Human CD4+ Memory T Cell Differentiation. J Immunol. 1997 Dec 15;159(12):6018-29. PubMed PMID: 9550400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Independent regulation of cutaneous lymphocyte-associated antigen expression and cytokine synthesis phenotype during human CD4+ memory T cell differentiation. AU - Teraki,Y, AU - Picker,L J, PY - 1998/4/29/pubmed PY - 1998/4/29/medline PY - 1998/4/29/entrez SP - 6018 EP - 29 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 159 IS - 12 N2 - Although considerable attention has been paid to the development of cytokine synthesis heterogeneity during memory T cell differentiation, little information is available on how this function is coregulated with homing receptor expression. The development of skin-homing, CD4+ memory T cells in the human provides an excellent model for such investigation, since 1) the skin supports both Th1- and Th2-predominant responses in different settings, and 2) the skin-homing capability of human memory T cells correlates with and appears to depend on expression of the skin-selective homing receptor cutaneous lymphocyte-associated Ag (CLA). In this study, we used multiparameter FACS analysis to examine expression of CLA vs IFN-gamma, IL-4, and IL-2 synthesis capabilities among fresh peripheral blood CD4+ memory T cells, and Th1 vs Th2 memory T cells generated in vitro from purified CD4+ naive precursors by cyclic activation in polarizing culture conditions. Among normal peripheral blood T cells, CLA expression was essentially identical among the IFN-gamma- vs IL-4-producing CD4+ memory subsets, clearly indicating the existence of in vivo mechanisms capable of producing both Th1 vs Th2 skin-homing T cells. In vitro differentiation of naive CD4+ T cells confirmed the independent regulation of CLA and all three cytokines examined, regulation that allowed differential production of IFN-gamma-, IL-4-, and IL-2-producing, CLA+ memory subsets. These studies also 1) demonstrated differences in regulatory factor activity depending on the differentiation status of the responding cell, and 2) revealed CLA expression to be much more rapidly reversible on established memory cells than cytokine synthesis capabilities. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9550400/Independent_regulation_of_cutaneous_lymphocyte_associated_antigen_expression_and_cytokine_synthesis_phenotype_during_human_CD4+_memory_T_cell_differentiation_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9550400 DB - PRIME DP - Unbound Medicine ER -