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Detection of circulating antigens of Parastrongylus cantonensis in human sera by dot-blot ELISA and sandwich ELISA using monoclonal antibody.

Abstract

A dot-blot ELISA was compared with a previously performed sandwich ELISA for the detection of Parastrongylus cantonensis antigens in sera from patients. Using the same monoclonal antibody and the same sera, 6 of 10 sera (60%) from parastronglyiasis patients were positive in dot-blot ELISA, whereas with sandwich ELISA, 5 of the same patient sera (50%) were positive. The specificity in both assays was 100% using 50 sera from patients with other parasitic diseases; of these, 10 each were from patients with cysticercosis, filariasis, gnathostomiasis, malaria and toxocariasis. The control group consisted of 53 sera from normal health Thais and Malaysians. The sensitivity of the assays was, however, slightly better with dot-blot ELISA and because it is simple, quick and cost-effective, it may be a test of choice for specific diagnosis of human parastrongyliasis.

Authors+Show Affiliations

Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bankok, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9561620

Citation

Eamsobhana, P, et al. "Detection of Circulating Antigens of Parastrongylus Cantonensis in Human Sera By Dot-blot ELISA and Sandwich ELISA Using Monoclonal Antibody." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 28, no. 3, 1997, pp. 624-8.
Eamsobhana P, Yong HS, Mak JW, et al. Detection of circulating antigens of Parastrongylus cantonensis in human sera by dot-blot ELISA and sandwich ELISA using monoclonal antibody. Southeast Asian J Trop Med Public Health. 1997;28(3):624-8.
Eamsobhana, P., Yong, H. S., Mak, J. W., & Wattanakulpanich, D. (1997). Detection of circulating antigens of Parastrongylus cantonensis in human sera by dot-blot ELISA and sandwich ELISA using monoclonal antibody. The Southeast Asian Journal of Tropical Medicine and Public Health, 28(3), 624-8.
Eamsobhana P, et al. Detection of Circulating Antigens of Parastrongylus Cantonensis in Human Sera By Dot-blot ELISA and Sandwich ELISA Using Monoclonal Antibody. Southeast Asian J Trop Med Public Health. 1997;28(3):624-8. PubMed PMID: 9561620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of circulating antigens of Parastrongylus cantonensis in human sera by dot-blot ELISA and sandwich ELISA using monoclonal antibody. AU - Eamsobhana,P, AU - Yong,H S, AU - Mak,J W, AU - Wattanakulpanich,D, PY - 1998/4/30/pubmed PY - 1998/4/30/medline PY - 1998/4/30/entrez SP - 624 EP - 8 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 28 IS - 3 N2 - A dot-blot ELISA was compared with a previously performed sandwich ELISA for the detection of Parastrongylus cantonensis antigens in sera from patients. Using the same monoclonal antibody and the same sera, 6 of 10 sera (60%) from parastronglyiasis patients were positive in dot-blot ELISA, whereas with sandwich ELISA, 5 of the same patient sera (50%) were positive. The specificity in both assays was 100% using 50 sera from patients with other parasitic diseases; of these, 10 each were from patients with cysticercosis, filariasis, gnathostomiasis, malaria and toxocariasis. The control group consisted of 53 sera from normal health Thais and Malaysians. The sensitivity of the assays was, however, slightly better with dot-blot ELISA and because it is simple, quick and cost-effective, it may be a test of choice for specific diagnosis of human parastrongyliasis. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/9561620/Detection_of_circulating_antigens_of_Parastrongylus_cantonensis_in_human_sera_by_dot_blot_ELISA_and_sandwich_ELISA_using_monoclonal_antibody_ DB - PRIME DP - Unbound Medicine ER -