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Estimation of galactose-1-phosphate in blood spotted on filter paper.
J Lab Clin Med. 1976 Sep; 88(3):515-9.JL

Abstract

A method is described for the enzymic estimation of galactose-1-phosphate (Gal-1-P) in blood which has been applied to filter paper and allowed to dry. The successful clinical management of patients with galactosemia depends upon exclusion of galactose from their diet. Earlier studies demonstrated that red cell Gal-1-P is a sensitive indicator of exposure of such patients to galactose. These earlier methods, however, required venipuncture, preparation of washed, packed red cells, and shipment of the sample in dry ice to a central laboratory. With the present method, capillary blood can be drawn by a nonphysician, applied to filter paper and mailed in a conventional envelope at ambient temperature. From this sample, the Gal-1-P content of the red cells can be determined, if the hematocrit is known. These conveniences should allow estimates of Gal-1-P at a frequency more appropirate for optimal dietary control.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

956702

Citation

Kirkman, H N., et al. "Estimation of Galactose-1-phosphate in Blood Spotted On Filter Paper." The Journal of Laboratory and Clinical Medicine, vol. 88, no. 3, 1976, pp. 515-9.
Kirkman HN, Lanier DC, Clemons EH, et al. Estimation of galactose-1-phosphate in blood spotted on filter paper. J Lab Clin Med. 1976;88(3):515-9.
Kirkman, H. N., Lanier, D. C., Clemons, E. H., & Sanderford, J. L. (1976). Estimation of galactose-1-phosphate in blood spotted on filter paper. The Journal of Laboratory and Clinical Medicine, 88(3), 515-9.
Kirkman HN, et al. Estimation of Galactose-1-phosphate in Blood Spotted On Filter Paper. J Lab Clin Med. 1976;88(3):515-9. PubMed PMID: 956702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estimation of galactose-1-phosphate in blood spotted on filter paper. AU - Kirkman,H N, AU - Lanier,D C, AU - Clemons,E H, AU - Sanderford,J L, PY - 1976/9/1/pubmed PY - 1976/9/1/medline PY - 1976/9/1/entrez SP - 515 EP - 9 JF - The Journal of laboratory and clinical medicine JO - J Lab Clin Med VL - 88 IS - 3 N2 - A method is described for the enzymic estimation of galactose-1-phosphate (Gal-1-P) in blood which has been applied to filter paper and allowed to dry. The successful clinical management of patients with galactosemia depends upon exclusion of galactose from their diet. Earlier studies demonstrated that red cell Gal-1-P is a sensitive indicator of exposure of such patients to galactose. These earlier methods, however, required venipuncture, preparation of washed, packed red cells, and shipment of the sample in dry ice to a central laboratory. With the present method, capillary blood can be drawn by a nonphysician, applied to filter paper and mailed in a conventional envelope at ambient temperature. From this sample, the Gal-1-P content of the red cells can be determined, if the hematocrit is known. These conveniences should allow estimates of Gal-1-P at a frequency more appropirate for optimal dietary control. SN - 0022-2143 UR - https://www.unboundmedicine.com/medline/citation/956702/Estimation_of_galactose_1_phosphate_in_blood_spotted_on_filter_paper_ DB - PRIME DP - Unbound Medicine ER -