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In vitro characteristics of myogenic satellite cells derived from the pectoralis major and biceps femoris muscles of the chicken.
Cytobios. 1997; 91(364):45-52.C

Abstract

Satellite cells were isolated from the pectoralis major (PM) and biceps femoris (BF) muscles of 5-week-old broiler chickens to compare growth and differentiation characteristics in vitro. BF cells proliferated at greater rates in the growth medium and were more responsive to the mitogenic effects of chicken serum than PM cells at all levels tested (p < or = 0.05). When low serum-containing medium was administered, the levels of creatine kinase, a marker of differentiation, increased at greater rates in PM cultures than in BF cultures (p < or = 0.05). Administration of increasing levels of fibroblast growth factor in serum-free medium resulted in similar responsiveness of the two lines to this mitogen. No differences were detected in rates of protein synthesis or degradation in myotube cultures from the two muscle sources. The results suggest that satellite cells derived from PM and BF muscles of the chicken have different responsiveness to serum mitogens.

Authors+Show Affiliations

McFarland DC
Department of Animal and Range Sciences, South Dakota State University, Brookings, South Dakota 57007, USA.
Gilkerson KK
No affiliation info available
Pesall JE
No affiliation info available
Ferrin NH
No affiliation info available
Wellenreiter RH
No affiliation info available

MeSH

AnimalsBlood ProteinsCell DifferentiationCell DivisionChickensCulture MediaFibroblast Growth FactorsInsulin-Like Growth Factor IMuscle Fibers, SkeletalPectoralis Muscles

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

9569620

Citation

McFarland, D C., et al. "In Vitro Characteristics of Myogenic Satellite Cells Derived From the Pectoralis Major and Biceps Femoris Muscles of the Chicken." Cytobios, vol. 91, no. 364, 1997, pp. 45-52.
McFarland DC, Gilkerson KK, Pesall JE, et al. In vitro characteristics of myogenic satellite cells derived from the pectoralis major and biceps femoris muscles of the chicken. Cytobios. 1997;91(364):45-52.
McFarland, D. C., Gilkerson, K. K., Pesall, J. E., Ferrin, N. H., & Wellenreiter, R. H. (1997). In vitro characteristics of myogenic satellite cells derived from the pectoralis major and biceps femoris muscles of the chicken. Cytobios, 91(364), 45-52.
McFarland DC, et al. In Vitro Characteristics of Myogenic Satellite Cells Derived From the Pectoralis Major and Biceps Femoris Muscles of the Chicken. Cytobios. 1997;91(364):45-52. PubMed PMID: 9569620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro characteristics of myogenic satellite cells derived from the pectoralis major and biceps femoris muscles of the chicken. AU - McFarland,D C, AU - Gilkerson,K K, AU - Pesall,J E, AU - Ferrin,N H, AU - Wellenreiter,R H, PY - 1997/1/1/pubmed PY - 1998/5/7/medline PY - 1997/1/1/entrez SP - 45 EP - 52 JF - Cytobios JO - Cytobios VL - 91 IS - 364 N2 - Satellite cells were isolated from the pectoralis major (PM) and biceps femoris (BF) muscles of 5-week-old broiler chickens to compare growth and differentiation characteristics in vitro. BF cells proliferated at greater rates in the growth medium and were more responsive to the mitogenic effects of chicken serum than PM cells at all levels tested (p < or = 0.05). When low serum-containing medium was administered, the levels of creatine kinase, a marker of differentiation, increased at greater rates in PM cultures than in BF cultures (p < or = 0.05). Administration of increasing levels of fibroblast growth factor in serum-free medium resulted in similar responsiveness of the two lines to this mitogen. No differences were detected in rates of protein synthesis or degradation in myotube cultures from the two muscle sources. The results suggest that satellite cells derived from PM and BF muscles of the chicken have different responsiveness to serum mitogens. SN - 0011-4529 UR - https://www.unboundmedicine.com/medline/citation/9569620/In_vitro_characteristics_of_myogenic_satellite_cells_derived_from_the_pectoralis_major_and_biceps_femoris_muscles_of_the_chicken_ DB - PRIME DP - Unbound Medicine ER -