Tags

Type your tag names separated by a space and hit enter

A bolus of recombinant human follicle stimulating hormone at midcycle induces periovulatory events following multiple follicular development in macaques.
Hum Reprod. 1998 Mar; 13(3):554-60.HR

Abstract

The efficacy of follicle stimulating hormone (FSH) as an alternative to luteinizing hormone (LH)/human chorionic gonadotrophin (HCG) for the initiation of periovulatory events in primate follicles is unknown. A single bolus of 2500 IU recombinant (r)-hFSH was compared to 1000 IU r-HCG for its ability to promote oocyte nuclear maturation and fertilization, granulosa cell luteinization and corpus luteum function following r-hFSH (60 IU/day) induction of multiple follicular development in rhesus monkeys. Following the r-hFSH bolus, bioactive luteinizing hormone concentrations were <3 ng/ml. Peak concentrations of serum FSH (1455+/-314 mIU/ml; mean+/-SEM) were attained 2-8 h after r-hFSH, and declined by 96 h. Bioactive HCG concentrations peaked between 2-8 h after r-HCG and remained > or = 100 ng/ml for >48 h, while immunoreactive FSH concentrations were at baseline. The proportion of oocytes resuming meiosis and undergoing in-vitro fertilization (IVF) were comparable for r-hFSH (89%; 47+/-19%) and r-HCG (88%; 50+/-17%). In-vitro progesterone production and expression of progesterone receptors in granulosa cells did not differ between groups. Peak concentrations of serum progesterone in the luteal phase were similar, but were lower 6-9 days post-FSH relative to HCG. Thus, a bolus of r-hFSH was equivalent to r-HCG for the reinitiation of oocyte meiosis, fertilization and granulosa cell luteinization, but a midcycle FSH surge did not sustain normal luteal function in primates.

Authors+Show Affiliations

Oregon Regional Primate Research Center, Beaverton 97006, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9572409

Citation

Zelinski-Wooten, M B., et al. "A Bolus of Recombinant Human Follicle Stimulating Hormone at Midcycle Induces Periovulatory Events Following Multiple Follicular Development in Macaques." Human Reproduction (Oxford, England), vol. 13, no. 3, 1998, pp. 554-60.
Zelinski-Wooten MB, Hutchison JS, Hess DL, et al. A bolus of recombinant human follicle stimulating hormone at midcycle induces periovulatory events following multiple follicular development in macaques. Hum Reprod. 1998;13(3):554-60.
Zelinski-Wooten, M. B., Hutchison, J. S., Hess, D. L., Wolf, D. P., & Stouffer, R. L. (1998). A bolus of recombinant human follicle stimulating hormone at midcycle induces periovulatory events following multiple follicular development in macaques. Human Reproduction (Oxford, England), 13(3), 554-60.
Zelinski-Wooten MB, et al. A Bolus of Recombinant Human Follicle Stimulating Hormone at Midcycle Induces Periovulatory Events Following Multiple Follicular Development in Macaques. Hum Reprod. 1998;13(3):554-60. PubMed PMID: 9572409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A bolus of recombinant human follicle stimulating hormone at midcycle induces periovulatory events following multiple follicular development in macaques. AU - Zelinski-Wooten,M B, AU - Hutchison,J S, AU - Hess,D L, AU - Wolf,D P, AU - Stouffer,R L, PY - 1998/5/8/pubmed PY - 1998/5/8/medline PY - 1998/5/8/entrez SP - 554 EP - 60 JF - Human reproduction (Oxford, England) JO - Hum Reprod VL - 13 IS - 3 N2 - The efficacy of follicle stimulating hormone (FSH) as an alternative to luteinizing hormone (LH)/human chorionic gonadotrophin (HCG) for the initiation of periovulatory events in primate follicles is unknown. A single bolus of 2500 IU recombinant (r)-hFSH was compared to 1000 IU r-HCG for its ability to promote oocyte nuclear maturation and fertilization, granulosa cell luteinization and corpus luteum function following r-hFSH (60 IU/day) induction of multiple follicular development in rhesus monkeys. Following the r-hFSH bolus, bioactive luteinizing hormone concentrations were <3 ng/ml. Peak concentrations of serum FSH (1455+/-314 mIU/ml; mean+/-SEM) were attained 2-8 h after r-hFSH, and declined by 96 h. Bioactive HCG concentrations peaked between 2-8 h after r-HCG and remained > or = 100 ng/ml for >48 h, while immunoreactive FSH concentrations were at baseline. The proportion of oocytes resuming meiosis and undergoing in-vitro fertilization (IVF) were comparable for r-hFSH (89%; 47+/-19%) and r-HCG (88%; 50+/-17%). In-vitro progesterone production and expression of progesterone receptors in granulosa cells did not differ between groups. Peak concentrations of serum progesterone in the luteal phase were similar, but were lower 6-9 days post-FSH relative to HCG. Thus, a bolus of r-hFSH was equivalent to r-HCG for the reinitiation of oocyte meiosis, fertilization and granulosa cell luteinization, but a midcycle FSH surge did not sustain normal luteal function in primates. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/9572409/A_bolus_of_recombinant_human_follicle_stimulating_hormone_at_midcycle_induces_periovulatory_events_following_multiple_follicular_development_in_macaques_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/13.3.554 DB - PRIME DP - Unbound Medicine ER -