Tags

Type your tag names separated by a space and hit enter

Nasopharyngeal colonization with nontypeable Haemophilus influenzae in chinchillas.
Infect Immun. 1998 May; 66(5):1973-80.II

Abstract

Colonization of the nasopharynx by a middle ear pathogen is the first step in the development of otitis media in humans. The establishment of an animal model of nasopharyngeal colonization would therefore be of great utility in assessing the potential protective ability of candidate vaccine antigens (especially adhesins) against otitis media. A chinchilla nasopharyngeal colonization model for nontypeable Haemophilus influenzae (NTHI) was developed with antibiotic-resistant strains. This model does not require coinfection with a virus. There was no significant difference in the efficiency of NTHI colonization between adult (1- to 2-year-old) and young (2- to 3-month-old) animals. However, the incidence of middle ear infection following nasopharyngeal colonization was significantly higher in young animals (83 to 89%) than in adult chinchillas (10 to 30%). Chinchillas that had recovered either from a previous middle ear infection caused by NTHI or from an infection by intranasal inoculation with NTHI were completely protected against nasopharyngeal colonization with a homologous strain and were found to be the best positive controls in protection studies. Systemic immunization of chinchillas with inactivated whole-cell preparations significantly protected animals not only against homologous NTHI colonization but also partially against heterologous NTHI infection. In all protected animals, significant serum anti-P6 and anti-HMW antibody responses were observed. The outer membrane P6 and high-molecular-weight (HMW) proteins appear to be promising candidate vaccine antigens to prevent nasopharyngeal colonization and middle ear infection caused by NTHI.

Authors+Show Affiliations

Research Center, Pasteur Merieux Connaught Canada, North York, Ontario. ypyang@ca.pmc-vacc.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9573078

Citation

Yang, Y P., et al. "Nasopharyngeal Colonization With Nontypeable Haemophilus Influenzae in Chinchillas." Infection and Immunity, vol. 66, no. 5, 1998, pp. 1973-80.
Yang YP, Loosmore SM, Underdown BJ, et al. Nasopharyngeal colonization with nontypeable Haemophilus influenzae in chinchillas. Infect Immun. 1998;66(5):1973-80.
Yang, Y. P., Loosmore, S. M., Underdown, B. J., & Klein, M. H. (1998). Nasopharyngeal colonization with nontypeable Haemophilus influenzae in chinchillas. Infection and Immunity, 66(5), 1973-80.
Yang YP, et al. Nasopharyngeal Colonization With Nontypeable Haemophilus Influenzae in Chinchillas. Infect Immun. 1998;66(5):1973-80. PubMed PMID: 9573078.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nasopharyngeal colonization with nontypeable Haemophilus influenzae in chinchillas. AU - Yang,Y P, AU - Loosmore,S M, AU - Underdown,B J, AU - Klein,M H, PY - 1998/5/9/pubmed PY - 1998/5/9/medline PY - 1998/5/9/entrez SP - 1973 EP - 80 JF - Infection and immunity JO - Infect Immun VL - 66 IS - 5 N2 - Colonization of the nasopharynx by a middle ear pathogen is the first step in the development of otitis media in humans. The establishment of an animal model of nasopharyngeal colonization would therefore be of great utility in assessing the potential protective ability of candidate vaccine antigens (especially adhesins) against otitis media. A chinchilla nasopharyngeal colonization model for nontypeable Haemophilus influenzae (NTHI) was developed with antibiotic-resistant strains. This model does not require coinfection with a virus. There was no significant difference in the efficiency of NTHI colonization between adult (1- to 2-year-old) and young (2- to 3-month-old) animals. However, the incidence of middle ear infection following nasopharyngeal colonization was significantly higher in young animals (83 to 89%) than in adult chinchillas (10 to 30%). Chinchillas that had recovered either from a previous middle ear infection caused by NTHI or from an infection by intranasal inoculation with NTHI were completely protected against nasopharyngeal colonization with a homologous strain and were found to be the best positive controls in protection studies. Systemic immunization of chinchillas with inactivated whole-cell preparations significantly protected animals not only against homologous NTHI colonization but also partially against heterologous NTHI infection. In all protected animals, significant serum anti-P6 and anti-HMW antibody responses were observed. The outer membrane P6 and high-molecular-weight (HMW) proteins appear to be promising candidate vaccine antigens to prevent nasopharyngeal colonization and middle ear infection caused by NTHI. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/9573078/Nasopharyngeal_colonization_with_nontypeable_Haemophilus_influenzae_in_chinchillas_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=9573078 DB - PRIME DP - Unbound Medicine ER -