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Characterization of an unusual sensor gene (virA) of Agrobacterium.
Gene. 1998 Apr 14; 210(2):307-14.GENE

Abstract

Previous studies have shown that the virulence(vir) genes of Agrobacterium tumefaciens strain KU12 are induced by a unique set of phenolic compounds that are non-functional in most strains of Agrobacterium. Further, strain KU12 is not induced by phenolic compounds that induce the vir genes in other strains. Previous studies have shown that these differences in inducing activity result from differences in the sensor protein for these signal molecules, the VirA protein. To gain some understanding of the basis for these differences in sensing ability, we sequenced the entire virA locus of pTiKU12, including its promoter region and compared this sequence with five different published virA sequences that respond in different ways to inducing compounds. The virA gene of KU12 is composed of an open single reading frame coding for 851 aa. At the aa level, the VirA protein of pTiKU12 is 45, 45, 49, 49 and 64% identical to the VirA proteins from pTiA6, pTi15955, pRiA4, pTiC58 and pTiAg162, respectively. The transcription start sites of pTiKU12 and pTiA6 virA genes differ significantly when mapped by primer extension. Unlike all other vir genes, except the virA gene of pTiAg162, pTiKU12 virA is constitutively expressed, and its synthesis is not induced by phenolic compounds. The lack of induction is accounted for by the fact that the promoter region does not have the conserved VirG-binding dodecadeoxynucleotide sequence (vir-box) that was previously identified in all promoter regions of inducible vir genes.

Authors+Show Affiliations

Department of Microbiology, University of Washington, Seattle 98195-7242, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9573388

Citation

Lee, Y W., et al. "Characterization of an Unusual Sensor Gene (virA) of Agrobacterium." Gene, vol. 210, no. 2, 1998, pp. 307-14.
Lee YW, Ha UH, Sim WS, et al. Characterization of an unusual sensor gene (virA) of Agrobacterium. Gene. 1998;210(2):307-14.
Lee, Y. W., Ha, U. H., Sim, W. S., & Nester, E. W. (1998). Characterization of an unusual sensor gene (virA) of Agrobacterium. Gene, 210(2), 307-14.
Lee YW, et al. Characterization of an Unusual Sensor Gene (virA) of Agrobacterium. Gene. 1998 Apr 14;210(2):307-14. PubMed PMID: 9573388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of an unusual sensor gene (virA) of Agrobacterium. AU - Lee,Y W, AU - Ha,U H, AU - Sim,W S, AU - Nester,E W, PY - 1998/5/9/pubmed PY - 1998/5/9/medline PY - 1998/5/9/entrez SP - 307 EP - 14 JF - Gene JO - Gene VL - 210 IS - 2 N2 - Previous studies have shown that the virulence(vir) genes of Agrobacterium tumefaciens strain KU12 are induced by a unique set of phenolic compounds that are non-functional in most strains of Agrobacterium. Further, strain KU12 is not induced by phenolic compounds that induce the vir genes in other strains. Previous studies have shown that these differences in inducing activity result from differences in the sensor protein for these signal molecules, the VirA protein. To gain some understanding of the basis for these differences in sensing ability, we sequenced the entire virA locus of pTiKU12, including its promoter region and compared this sequence with five different published virA sequences that respond in different ways to inducing compounds. The virA gene of KU12 is composed of an open single reading frame coding for 851 aa. At the aa level, the VirA protein of pTiKU12 is 45, 45, 49, 49 and 64% identical to the VirA proteins from pTiA6, pTi15955, pRiA4, pTiC58 and pTiAg162, respectively. The transcription start sites of pTiKU12 and pTiA6 virA genes differ significantly when mapped by primer extension. Unlike all other vir genes, except the virA gene of pTiAg162, pTiKU12 virA is constitutively expressed, and its synthesis is not induced by phenolic compounds. The lack of induction is accounted for by the fact that the promoter region does not have the conserved VirG-binding dodecadeoxynucleotide sequence (vir-box) that was previously identified in all promoter regions of inducible vir genes. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/9573388/Characterization_of_an_unusual_sensor_gene__virA__of_Agrobacterium_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378111998000821 DB - PRIME DP - Unbound Medicine ER -