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A T-DNA from the Agrobacterium tumefaciens limited-host-range strain AB2/73 contains a single oncogene.
Mol Plant Microbe Interact. 1998 May; 11(5):335-42.MP

Abstract

Agrobacterium tumefaciens strain AB2/73 isolated from Lippia canescens has been described as a limited-host-range strain. Its tumor-inducing (Ti) plasmid has been found to lack DNA homology to known T-DNAs (L. Unger, S. F. Ziegler, G. A. Huffman, V. C. Knauf, R. Peet, L. W. Moore, M. P. Gordon, and E. W. Nester. J. Bacteriol. 164:723-730, 1985). We have isolated a T-DNA from AB2/73 by using a heterologous border sequence as a probe. The AB2/73 T-DNA sequence (3,504 bp) is flanked by canonical border sequences, has no detectable DNA homology with other T-DNAs, and contains only two genes: lsn (Lippia strain nopaline synthaselike gene) and lso (Lippia strain oncogene). The lso gene induces nondifferentiating tumors on a limited number of hosts when transferred by a Ti plasmid from a wide-host-range strain. Part of the predicted Lso protein is weakly homologous to other Agrobacterium oncoproteins encoded by rolB, rolB, orf13, gene e, gene 5, and gene 3'. A 28-kb fragment corresponding to the virA to virE region was cloned by using a heterologous vir fragment as probe. The AB2/73 vir region is homologous to most of the C58 virulence region; however, the virA gene is most related to the virA gene of the Agrobacterium vitis limited-host-range strain Ag162.

Authors+Show Affiliations

Department of Cell Biology, Plant Molecular Biology Institute of the C.N.R.S., Strasbourg, France. leon.otten@ibmp-ulp.u-strasbg.frNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9574502

Citation

Otten, L, and J Schmidt. "A T-DNA From the Agrobacterium Tumefaciens Limited-host-range Strain AB2/73 Contains a Single Oncogene." Molecular Plant-microbe Interactions : MPMI, vol. 11, no. 5, 1998, pp. 335-42.
Otten L, Schmidt J. A T-DNA from the Agrobacterium tumefaciens limited-host-range strain AB2/73 contains a single oncogene. Mol Plant Microbe Interact. 1998;11(5):335-42.
Otten, L., & Schmidt, J. (1998). A T-DNA from the Agrobacterium tumefaciens limited-host-range strain AB2/73 contains a single oncogene. Molecular Plant-microbe Interactions : MPMI, 11(5), 335-42.
Otten L, Schmidt J. A T-DNA From the Agrobacterium Tumefaciens Limited-host-range Strain AB2/73 Contains a Single Oncogene. Mol Plant Microbe Interact. 1998;11(5):335-42. PubMed PMID: 9574502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A T-DNA from the Agrobacterium tumefaciens limited-host-range strain AB2/73 contains a single oncogene. AU - Otten,L, AU - Schmidt,J, PY - 1998/5/9/pubmed PY - 1998/5/9/medline PY - 1998/5/9/entrez SP - 335 EP - 42 JF - Molecular plant-microbe interactions : MPMI JO - Mol. Plant Microbe Interact. VL - 11 IS - 5 N2 - Agrobacterium tumefaciens strain AB2/73 isolated from Lippia canescens has been described as a limited-host-range strain. Its tumor-inducing (Ti) plasmid has been found to lack DNA homology to known T-DNAs (L. Unger, S. F. Ziegler, G. A. Huffman, V. C. Knauf, R. Peet, L. W. Moore, M. P. Gordon, and E. W. Nester. J. Bacteriol. 164:723-730, 1985). We have isolated a T-DNA from AB2/73 by using a heterologous border sequence as a probe. The AB2/73 T-DNA sequence (3,504 bp) is flanked by canonical border sequences, has no detectable DNA homology with other T-DNAs, and contains only two genes: lsn (Lippia strain nopaline synthaselike gene) and lso (Lippia strain oncogene). The lso gene induces nondifferentiating tumors on a limited number of hosts when transferred by a Ti plasmid from a wide-host-range strain. Part of the predicted Lso protein is weakly homologous to other Agrobacterium oncoproteins encoded by rolB, rolB, orf13, gene e, gene 5, and gene 3'. A 28-kb fragment corresponding to the virA to virE region was cloned by using a heterologous vir fragment as probe. The AB2/73 vir region is homologous to most of the C58 virulence region; however, the virA gene is most related to the virA gene of the Agrobacterium vitis limited-host-range strain Ag162. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/9574502/A_T_DNA_from_the_Agrobacterium_tumefaciens_limited_host_range_strain_AB2/73_contains_a_single_oncogene_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/MPMI.1998.11.5.335?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -