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The presence and characterization of a virF gene on Agrobacterium vitis Ti plasmids.
Mol Plant Microbe Interact. 1998 May; 11(5):429-33.MP

Abstract

Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plasmids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the pTiAg57 virF is inducible by the plant phenolic compound acetosyringone through the presence of a vir-box consensus sequence in its promoter region. The VirF protein from pTiAg57 can complement octopine A. tumefaciens strains deleted for virF as shown by tumor formation on N. glauca.

Authors+Show Affiliations

Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9574510

Citation

Schrammeijer, B, et al. "The Presence and Characterization of a virF Gene On Agrobacterium Vitis Ti Plasmids." Molecular Plant-microbe Interactions : MPMI, vol. 11, no. 5, 1998, pp. 429-33.
Schrammeijer B, Hemelaar J, Hooykaas PJ. The presence and characterization of a virF gene on Agrobacterium vitis Ti plasmids. Mol Plant Microbe Interact. 1998;11(5):429-33.
Schrammeijer, B., Hemelaar, J., & Hooykaas, P. J. (1998). The presence and characterization of a virF gene on Agrobacterium vitis Ti plasmids. Molecular Plant-microbe Interactions : MPMI, 11(5), 429-33.
Schrammeijer B, Hemelaar J, Hooykaas PJ. The Presence and Characterization of a virF Gene On Agrobacterium Vitis Ti Plasmids. Mol Plant Microbe Interact. 1998;11(5):429-33. PubMed PMID: 9574510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The presence and characterization of a virF gene on Agrobacterium vitis Ti plasmids. AU - Schrammeijer,B, AU - Hemelaar,J, AU - Hooykaas,P J, PY - 1998/5/9/pubmed PY - 1998/5/9/medline PY - 1998/5/9/entrez SP - 429 EP - 33 JF - Molecular plant-microbe interactions : MPMI JO - Mol. Plant Microbe Interact. VL - 11 IS - 5 N2 - Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N. glauca a host plant for these strains, indicating that the VirF protein is a host-range determinant. Here we show the presence of a virF locus not only on the Agrobacterium vitis octopine/cucumopine plasmids pTiAg57 and pTiTm4, but also on the nopaline Ti plasmids pTiAT1, pTiAT66a, and pTiAT66b. On the octopine Ti plasmids from A. tumefaciens the virF gene is located between the virE locus and the left border of the T-region. In contrast, the virF gene on Ti plasmids of A. vitis is located at the very left end of the vir-region near the virA locus. The virF gene of pTiAg57 has been sequenced and codes for a protein of 202 amino acids with a molecular mass of 22,280 Da. Comparison showed that the virF gene from A. vitis strain Ag57 is almost identical to that from A. tumefaciens octopine strains. The transcription of the pTiAg57 virF is inducible by the plant phenolic compound acetosyringone through the presence of a vir-box consensus sequence in its promoter region. The VirF protein from pTiAg57 can complement octopine A. tumefaciens strains deleted for virF as shown by tumor formation on N. glauca. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/9574510/The_presence_and_characterization_of_a_virF_gene_on_Agrobacterium_vitis_Ti_plasmids_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/MPMI.1998.11.5.429?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -