Citation
Wang, P, et al. "Selective Inhibition of IL-5 Receptor Alpha-chain Gene Transcription By IL-5, IL-3, and Granulocyte-macrophage Colony-stimulating Factor in Human Blood Eosinophils." Journal of Immunology (Baltimore, Md. : 1950), vol. 160, no. 9, 1998, pp. 4427-32.
Wang P, Wu P, Cheewatrakoolpong B, et al. Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils. J Immunol. 1998;160(9):4427-32.
Wang, P., Wu, P., Cheewatrakoolpong, B., Myers, J. G., Egan, R. W., & Billah, M. M. (1998). Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils. Journal of Immunology (Baltimore, Md. : 1950), 160(9), 4427-32.
Wang P, et al. Selective Inhibition of IL-5 Receptor Alpha-chain Gene Transcription By IL-5, IL-3, and Granulocyte-macrophage Colony-stimulating Factor in Human Blood Eosinophils. J Immunol. 1998 May 1;160(9):4427-32. PubMed PMID: 9574547.
TY - JOUR
T1 - Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils.
AU - Wang,P,
AU - Wu,P,
AU - Cheewatrakoolpong,B,
AU - Myers,J G,
AU - Egan,R W,
AU - Billah,M M,
PY - 1998/5/9/pubmed
PY - 1998/5/9/medline
PY - 1998/5/9/entrez
SP - 4427
EP - 32
JF - Journal of immunology (Baltimore, Md. : 1950)
JO - J Immunol
VL - 160
IS - 9
N2 - High affinity receptor for IL-5 (IL-5R), a predominant eosinophil maturation factor, is composed of an IL-5-binding alpha-chain (IL-5R alpha) and a signal-transducing beta-chain that is shared by IL-3 and granulocyte-macrophage CSF (GM-CSF) receptors (IL-3R and GM-CSFR). By Northern blot analysis of mRNAs obtained from normal human blood eosinophils, we show in this report that the hematopoietic cytokines IL-5, IL-3, and GM-CSF down-regulate IL-5R alpha mRNA while up-regulating alpha-chain mRNAs for both IL-3R and GM-CSFR as well as the beta-chain mRNA. More detailed characterization reveals that the down-regulation of IL-5R alpha mRNA is specific to IL-3, IL-5, and GM-CSF; occurs very rapidly (reaching maximum inhibition within 2 h); is cytokine dose dependent; and does not require protein synthesis. Nuclear run-on and mRNA stability experiments demonstrate that cytokine-induced inhibition of IL-5R alpha mRNA accumulation occurs at the level of IL-5R alpha gene transcription, whereas enhanced accumulation of mRNAs for IL-3R alpha and the beta-chain results from reduced mRNA degradation. We suggest from these experiments that in human blood eosinophils, IL-5R alpha gene transcription and IL-5R alpha mRNA metabolism can be regulated by mechanisms that are distinct from those used for IL-3R alpha and GM-CSFR alpha.
SN - 0022-1767
UR - https://www.unboundmedicine.com/medline/citation/9574547/Selective_inhibition_of_IL_5_receptor_alpha_chain_gene_transcription_by_IL_5_IL_3_and_granulocyte_macrophage_colony_stimulating_factor_in_human_blood_eosinophils_
L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9574547
DB - PRIME
DP - Unbound Medicine
ER -