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Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils.
J Immunol. 1998 May 01; 160(9):4427-32.JI

Abstract

High affinity receptor for IL-5 (IL-5R), a predominant eosinophil maturation factor, is composed of an IL-5-binding alpha-chain (IL-5R alpha) and a signal-transducing beta-chain that is shared by IL-3 and granulocyte-macrophage CSF (GM-CSF) receptors (IL-3R and GM-CSFR). By Northern blot analysis of mRNAs obtained from normal human blood eosinophils, we show in this report that the hematopoietic cytokines IL-5, IL-3, and GM-CSF down-regulate IL-5R alpha mRNA while up-regulating alpha-chain mRNAs for both IL-3R and GM-CSFR as well as the beta-chain mRNA. More detailed characterization reveals that the down-regulation of IL-5R alpha mRNA is specific to IL-3, IL-5, and GM-CSF; occurs very rapidly (reaching maximum inhibition within 2 h); is cytokine dose dependent; and does not require protein synthesis. Nuclear run-on and mRNA stability experiments demonstrate that cytokine-induced inhibition of IL-5R alpha mRNA accumulation occurs at the level of IL-5R alpha gene transcription, whereas enhanced accumulation of mRNAs for IL-3R alpha and the beta-chain results from reduced mRNA degradation. We suggest from these experiments that in human blood eosinophils, IL-5R alpha gene transcription and IL-5R alpha mRNA metabolism can be regulated by mechanisms that are distinct from those used for IL-3R alpha and GM-CSFR alpha.

Authors+Show Affiliations

Allergy Department, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA. peng.wang@spcorp.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9574547

Citation

Wang, P, et al. "Selective Inhibition of IL-5 Receptor Alpha-chain Gene Transcription By IL-5, IL-3, and Granulocyte-macrophage Colony-stimulating Factor in Human Blood Eosinophils." Journal of Immunology (Baltimore, Md. : 1950), vol. 160, no. 9, 1998, pp. 4427-32.
Wang P, Wu P, Cheewatrakoolpong B, et al. Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils. J Immunol. 1998;160(9):4427-32.
Wang, P., Wu, P., Cheewatrakoolpong, B., Myers, J. G., Egan, R. W., & Billah, M. M. (1998). Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils. Journal of Immunology (Baltimore, Md. : 1950), 160(9), 4427-32.
Wang P, et al. Selective Inhibition of IL-5 Receptor Alpha-chain Gene Transcription By IL-5, IL-3, and Granulocyte-macrophage Colony-stimulating Factor in Human Blood Eosinophils. J Immunol. 1998 May 1;160(9):4427-32. PubMed PMID: 9574547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils. AU - Wang,P, AU - Wu,P, AU - Cheewatrakoolpong,B, AU - Myers,J G, AU - Egan,R W, AU - Billah,M M, PY - 1998/5/9/pubmed PY - 1998/5/9/medline PY - 1998/5/9/entrez SP - 4427 EP - 32 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 160 IS - 9 N2 - High affinity receptor for IL-5 (IL-5R), a predominant eosinophil maturation factor, is composed of an IL-5-binding alpha-chain (IL-5R alpha) and a signal-transducing beta-chain that is shared by IL-3 and granulocyte-macrophage CSF (GM-CSF) receptors (IL-3R and GM-CSFR). By Northern blot analysis of mRNAs obtained from normal human blood eosinophils, we show in this report that the hematopoietic cytokines IL-5, IL-3, and GM-CSF down-regulate IL-5R alpha mRNA while up-regulating alpha-chain mRNAs for both IL-3R and GM-CSFR as well as the beta-chain mRNA. More detailed characterization reveals that the down-regulation of IL-5R alpha mRNA is specific to IL-3, IL-5, and GM-CSF; occurs very rapidly (reaching maximum inhibition within 2 h); is cytokine dose dependent; and does not require protein synthesis. Nuclear run-on and mRNA stability experiments demonstrate that cytokine-induced inhibition of IL-5R alpha mRNA accumulation occurs at the level of IL-5R alpha gene transcription, whereas enhanced accumulation of mRNAs for IL-3R alpha and the beta-chain results from reduced mRNA degradation. We suggest from these experiments that in human blood eosinophils, IL-5R alpha gene transcription and IL-5R alpha mRNA metabolism can be regulated by mechanisms that are distinct from those used for IL-3R alpha and GM-CSFR alpha. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9574547/Selective_inhibition_of_IL_5_receptor_alpha_chain_gene_transcription_by_IL_5_IL_3_and_granulocyte_macrophage_colony_stimulating_factor_in_human_blood_eosinophils_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9574547 DB - PRIME DP - Unbound Medicine ER -