TY - JOUR T1 - Molecular and serologic survey of Orientia tsutsugamushi infection among field rodents in southern Cholla Province, Korea. AU - Song,H J, AU - Seong,S Y, AU - Huh,M S, AU - Park,S G, AU - Jang,W J, AU - Kee,S H, AU - Kim,K H, AU - Kim,S C, AU - Choi,M S, AU - Kim,I S, AU - Chang,W H, PY - 1998/5/9/pubmed PY - 1998/5/9/medline PY - 1998/5/9/entrez SP - 513 EP - 8 JF - The American journal of tropical medicine and hygiene JO - Am J Trop Med Hyg VL - 58 IS - 4 N2 - Field rodents were collected from six areas in southern Cholla Province, Korea from October to December 1993. Twenty-eight (24%) of the 119 Apodemus agrarius were seropositive (> 1:10) for Orientia tsutsugamushi by the passive hemagglutination assay (PHA). Of the seropositive cases, 11 specimens had antibody titers greater than 1:80. No seropositive specimens were found among the eight Crocidura lasiura collected. On the other hand, the polymerase chain reaction (PCR) amplified about 520 basepairs of a gene encoding the 56-kD protein from the genomic DNA of 12 strains of O. tsutsugamushi tested. This target DNA sequence was amplified from the 11 (8.7%) blood specimens of A. agrarius, and one of the eight C. lasiura also showed evidence of O. tsutsugamushi infection by PCR. Only one of the PCR-positive samples was also PHA-positive. These results suggest that the PCR combined with a serologic assay more accurately detects the degree of infection of rodents with rickettsiae-causing scrub typhus in epidemiologic surveys. SN - 0002-9637 UR - https://www.unboundmedicine.com/medline/citation/9574801/Molecular_and_serologic_survey_of_Orientia_tsutsugamushi_infection_among_field_rodents_in_southern_Cholla_Province_Korea_ L2 - https://ajtmh.org/doi/10.4269/ajtmh.1998.58.513 DB - PRIME DP - Unbound Medicine ER -