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Increased frequencies of interleukin-2- and interferon-gamma-producing T cells in patients with active Behçet's disease.
Invest Ophthalmol Vis Sci. 1998 May; 39(6):996-1004.IO

Abstract

PURPOSE

To elucidate the profile of cytokine-producing T cells in patients with active or inactive Behçet's disease (BD), the frequencies of type 1 (Interleukin- [IL] 2, interferon-gamma [IFN-gamma]) and type 2 (IL-4) cytokine-producing CD4+ and CD8+ cells in peripheral blood were investigated, and the effect of immunosuppressive drugs on the profile of cytokine-producing cells was evaluated.

METHODS

Fifty-two patients with BD (32 with active and 20 with inactive BD) and 33 healthy control subjects were the subjects in this study. Patients were or were not treated with immunosuppressive drugs. Peripheral blood mononuclear cells were fixed, permeabilized, and stained for intracellular cytokines in combination with cell surface markers CD4 and CD8 for flow cytometric analysis.

RESULTS

In nontreated patients with BD, the frequencies of IL-2- and IFN-gamma-producing CD4 and CD8+ cells in active patients were significantly higher than those in inactive patients. Conversely, the frequencies of IL-4 producing CD4+ and CD8+ cells in nontreated patients with active BD were comparable with those in patients with inactive disease and in control subjects. Patients with inactive BD who were treated with immunosuppressive drugs showed significantly lower frequencies of IL-2- and IFN-gamma-producing CD4+ and CD8+ cells than did treated patients with active BD.

CONCLUSIONS

The frequencies of type 1 cytokine-producing CD4+ and CD8+ cells increased in patients with active BD. Effective immunosuppressive treatments decreased the population of type 1 cytokine-producing CD4+ and CD8+ cells. These results suggest that type 1 cytokine-producing cells play an important role in the immunopathogenesis of the inflammation in BD.

Authors+Show Affiliations

Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9579479

Citation

Sugi-Ikai, N, et al. "Increased Frequencies of Interleukin-2- and Interferon-gamma-producing T Cells in Patients With Active Behçet's Disease." Investigative Ophthalmology & Visual Science, vol. 39, no. 6, 1998, pp. 996-1004.
Sugi-Ikai N, Nakazawa M, Nakamura S, et al. Increased frequencies of interleukin-2- and interferon-gamma-producing T cells in patients with active Behçet's disease. Invest Ophthalmol Vis Sci. 1998;39(6):996-1004.
Sugi-Ikai, N., Nakazawa, M., Nakamura, S., Ohno, S., & Minami, M. (1998). Increased frequencies of interleukin-2- and interferon-gamma-producing T cells in patients with active Behçet's disease. Investigative Ophthalmology & Visual Science, 39(6), 996-1004.
Sugi-Ikai N, et al. Increased Frequencies of Interleukin-2- and Interferon-gamma-producing T Cells in Patients With Active Behçet's Disease. Invest Ophthalmol Vis Sci. 1998;39(6):996-1004. PubMed PMID: 9579479.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased frequencies of interleukin-2- and interferon-gamma-producing T cells in patients with active Behçet's disease. AU - Sugi-Ikai,N, AU - Nakazawa,M, AU - Nakamura,S, AU - Ohno,S, AU - Minami,M, PY - 1998/5/14/pubmed PY - 1998/5/14/medline PY - 1998/5/14/entrez SP - 996 EP - 1004 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 39 IS - 6 N2 - PURPOSE: To elucidate the profile of cytokine-producing T cells in patients with active or inactive Behçet's disease (BD), the frequencies of type 1 (Interleukin- [IL] 2, interferon-gamma [IFN-gamma]) and type 2 (IL-4) cytokine-producing CD4+ and CD8+ cells in peripheral blood were investigated, and the effect of immunosuppressive drugs on the profile of cytokine-producing cells was evaluated. METHODS: Fifty-two patients with BD (32 with active and 20 with inactive BD) and 33 healthy control subjects were the subjects in this study. Patients were or were not treated with immunosuppressive drugs. Peripheral blood mononuclear cells were fixed, permeabilized, and stained for intracellular cytokines in combination with cell surface markers CD4 and CD8 for flow cytometric analysis. RESULTS: In nontreated patients with BD, the frequencies of IL-2- and IFN-gamma-producing CD4 and CD8+ cells in active patients were significantly higher than those in inactive patients. Conversely, the frequencies of IL-4 producing CD4+ and CD8+ cells in nontreated patients with active BD were comparable with those in patients with inactive disease and in control subjects. Patients with inactive BD who were treated with immunosuppressive drugs showed significantly lower frequencies of IL-2- and IFN-gamma-producing CD4+ and CD8+ cells than did treated patients with active BD. CONCLUSIONS: The frequencies of type 1 cytokine-producing CD4+ and CD8+ cells increased in patients with active BD. Effective immunosuppressive treatments decreased the population of type 1 cytokine-producing CD4+ and CD8+ cells. These results suggest that type 1 cytokine-producing cells play an important role in the immunopathogenesis of the inflammation in BD. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/9579479/Increased_frequencies_of_interleukin_2__and_interferon_gamma_producing_T_cells_in_patients_with_active_Behçet's_disease_ L2 - https://iovs.arvojournals.org/article.aspx?volume=39&issue=6&page=996 DB - PRIME DP - Unbound Medicine ER -