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Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53.
Mol Pharmacol. 1998 May; 53(5):819-26.MP

Abstract

We investigated the roles of p53 and Bcl-2 homologues in the induction of apoptosis by cisplatin and paclitaxel in wild-type p53-expressing human ovarian carcinoma cells and cisplatin-resistant derivatives that have lost p53 function. Cisplatin induced apoptosis in parental A2780 but not in cisplatin-resistant A2780/cp70 cells, whereas paclitaxel induced apoptosis in both cell lines. Immunoprecipitation of p53 using antibodies specific for p53 conformation (pAb 1620 and pAb 240) showed that there were no relative changes in p53 conformation before and after cisplatin treatment in either cell line. A2780/cp70 cells have lost p53 function, yet they have wild-type p53 gene sequence. However, A2780/cp70 cells constitutively express more p53 in a form detected by pAb 240, an antibody that also detects mutant conformations of p53 that are transcriptionally inactive. There were no changes in levels of Bcl-2, Bcl-XL, or 24-kDa Bax over 72 hr after exposure to cisplatin or paclitaxel, but each agent led to up-regulation of Bak and 21-kDa Bax in A2780 cells. Paclitaxel, but not cisplatin, increased Bak and 21-kDa Bax levels in A2780/cp70 cells. These data suggest that apoptosis in A2780 and A2780/cp70 is associated with an increased level of Bak and 21 kDa Bax after drug-induced damage and that functional p53 may be required for this effect after cisplatin but not after paclitaxel.

Authors+Show Affiliations

Cancer Research Campaign Molecular and Cellular Pharmacology Research Group, School of Biological Sciences, University of Manchester, Manchester, M13 9PT, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9584207

Citation

Jones, N A., et al. "Cisplatin- and Paclitaxel-induced Apoptosis of Ovarian Carcinoma Cells and the Relationship Between Bax and Bak Up-regulation and the Functional Status of P53." Molecular Pharmacology, vol. 53, no. 5, 1998, pp. 819-26.
Jones NA, Turner J, McIlwrath AJ, et al. Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53. Mol Pharmacol. 1998;53(5):819-26.
Jones, N. A., Turner, J., McIlwrath, A. J., Brown, R., & Dive, C. (1998). Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53. Molecular Pharmacology, 53(5), 819-26.
Jones NA, et al. Cisplatin- and Paclitaxel-induced Apoptosis of Ovarian Carcinoma Cells and the Relationship Between Bax and Bak Up-regulation and the Functional Status of P53. Mol Pharmacol. 1998;53(5):819-26. PubMed PMID: 9584207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53. AU - Jones,N A, AU - Turner,J, AU - McIlwrath,A J, AU - Brown,R, AU - Dive,C, PY - 1998/6/20/pubmed PY - 1998/6/20/medline PY - 1998/6/20/entrez SP - 819 EP - 26 JF - Molecular pharmacology JO - Mol Pharmacol VL - 53 IS - 5 N2 - We investigated the roles of p53 and Bcl-2 homologues in the induction of apoptosis by cisplatin and paclitaxel in wild-type p53-expressing human ovarian carcinoma cells and cisplatin-resistant derivatives that have lost p53 function. Cisplatin induced apoptosis in parental A2780 but not in cisplatin-resistant A2780/cp70 cells, whereas paclitaxel induced apoptosis in both cell lines. Immunoprecipitation of p53 using antibodies specific for p53 conformation (pAb 1620 and pAb 240) showed that there were no relative changes in p53 conformation before and after cisplatin treatment in either cell line. A2780/cp70 cells have lost p53 function, yet they have wild-type p53 gene sequence. However, A2780/cp70 cells constitutively express more p53 in a form detected by pAb 240, an antibody that also detects mutant conformations of p53 that are transcriptionally inactive. There were no changes in levels of Bcl-2, Bcl-XL, or 24-kDa Bax over 72 hr after exposure to cisplatin or paclitaxel, but each agent led to up-regulation of Bak and 21-kDa Bax in A2780 cells. Paclitaxel, but not cisplatin, increased Bak and 21-kDa Bax levels in A2780/cp70 cells. These data suggest that apoptosis in A2780 and A2780/cp70 is associated with an increased level of Bak and 21 kDa Bax after drug-induced damage and that functional p53 may be required for this effect after cisplatin but not after paclitaxel. SN - 0026-895X UR - https://www.unboundmedicine.com/medline/citation/9584207/Cisplatin__and_paclitaxel_induced_apoptosis_of_ovarian_carcinoma_cells_and_the_relationship_between_bax_and_bak_up_regulation_and_the_functional_status_of_p53_ L2 - http://molpharm.aspetjournals.org/cgi/pmidlookup?view=long&pmid=9584207 DB - PRIME DP - Unbound Medicine ER -