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A mechanism for unsplicing and exon skipping in human alpha- and beta-globin mutant pre-mRNA splicing.

Abstract

In both of normal human alpha- and beta-globin pre-mRNAs, two introns are spliced correctly, giving mRNA products of (exon 1)-(exon 2)-(exon 3). If 5'-splice site of the second intron is mutated, mRNA of the beta-globin is composed of (exon 1)-(exon 3), skipping the whole exon 2 sequence. However, if 5'-splice site of the second intron is mutated in the alpha-globin, mRNA is (exon 1)-(exon 2)-(intron 2)-(exon 3), unsplicing intron 2. In both of the alpha- and beta-globin pre-mRNAs, strengths of 3'-splice signals of the first and second introns were calculated by the quantification method reported previously, which explains a reason for exon skipping and unsplicing in terms of relative strengths of those signals.

Authors+Show Affiliations

Department of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9586060

Citation

Iida, Y. "A Mechanism for Unsplicing and Exon Skipping in Human Alpha- and Beta-globin Mutant pre-mRNA Splicing." Nucleic Acids Symposium Series, 1997, pp. 183-4.
Iida Y. A mechanism for unsplicing and exon skipping in human alpha- and beta-globin mutant pre-mRNA splicing. Nucleic Acids Symp Ser. 1997.
Iida, Y. (1997). A mechanism for unsplicing and exon skipping in human alpha- and beta-globin mutant pre-mRNA splicing. Nucleic Acids Symposium Series, (37), 183-4.
Iida Y. A Mechanism for Unsplicing and Exon Skipping in Human Alpha- and Beta-globin Mutant pre-mRNA Splicing. Nucleic Acids Symp Ser. 1997;(37)183-4. PubMed PMID: 9586060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A mechanism for unsplicing and exon skipping in human alpha- and beta-globin mutant pre-mRNA splicing. A1 - Iida,Y, PY - 1997/1/1/pubmed PY - 1998/5/20/medline PY - 1997/1/1/entrez SP - 183 EP - 4 JF - Nucleic acids symposium series JO - Nucleic Acids Symp Ser IS - 37 N2 - In both of normal human alpha- and beta-globin pre-mRNAs, two introns are spliced correctly, giving mRNA products of (exon 1)-(exon 2)-(exon 3). If 5'-splice site of the second intron is mutated, mRNA of the beta-globin is composed of (exon 1)-(exon 3), skipping the whole exon 2 sequence. However, if 5'-splice site of the second intron is mutated in the alpha-globin, mRNA is (exon 1)-(exon 2)-(intron 2)-(exon 3), unsplicing intron 2. In both of the alpha- and beta-globin pre-mRNAs, strengths of 3'-splice signals of the first and second introns were calculated by the quantification method reported previously, which explains a reason for exon skipping and unsplicing in terms of relative strengths of those signals. SN - 0261-3166 UR - https://www.unboundmedicine.com/medline/citation/9586060/A_mechanism_for_unsplicing_and_exon_skipping_in_human_alpha__and_beta_globin_mutant_pre_mRNA_splicing_ DB - PRIME DP - Unbound Medicine ER -