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Functional analysis of copper homeostasis in cell culture models: a new perspective on internal copper transport.
Am J Clin Nutr. 1998 05; 67(5 Suppl):988S-995S.AJ

Abstract

The movement of copper ions across membrane barriers of vital organs and tissues is a priority topic in nutrition and one for which there continues to be little understanding of the mechanism. Reports of membrane-bound, copper-transporting adenosine triphosphatases (Cu-ATPases) selective for copper ions have brought new focus to the problem and prompted fresh ideas. Using a cell culture model approach, we attempted to learn whether transport into and out of cells depends on a Cu-ATPase. Measurement of transport kinetics in fibroblasts, brain glial cells, neuroblastoma cells, and placental cells showed differences in the rates of copper uptake and response to sulfhydryl reagents. BeWo cells, a human choriocarcinoma placental cell line, behaved as did Menkes fibroblasts by avidly absorbing copper but not releasing copper to the immediate environment. Further tests showed that BeWo cells did not express the transcript for the membrane-bound Cu-ATPase that has been identified with Menkes syndrome. Transcript induction, however, was achieved by growing BeWo cells on porous filters that allowed apical and basolateral surfaces to form. With transcript expression, the cells showed a capacity to release copper into the medium. BeWo cells also synthesized a form of ceruloplasmin whose structure differed from that of the plasma protein and hence may be a product of a different gene. BeWo cells may also express the gene for Wilson disease, thus linking Menkes and Wilson proteins to maternal delivery of copper. We constructed a model in which both ATPases work in concert in a vesicle-based transport mechanism. The vesicle model may help us understand the transport of copper across the placenta and all cells in general.

Authors+Show Affiliations

Department of Biochemistry and Biophysics and the Faculty of Nutrition, Texas A&M University, College Station 77843-2128, USA. eharris@bioch.tamu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
Review

Language

eng

PubMed ID

9587141

Citation

Harris, E D., et al. "Functional Analysis of Copper Homeostasis in Cell Culture Models: a New Perspective On Internal Copper Transport." The American Journal of Clinical Nutrition, vol. 67, no. 5 Suppl, 1998, 988S-995S.
Harris ED, Qian Y, Tiffany-Castiglioni E, et al. Functional analysis of copper homeostasis in cell culture models: a new perspective on internal copper transport. Am J Clin Nutr. 1998;67(5 Suppl):988S-995S.
Harris, E. D., Qian, Y., Tiffany-Castiglioni, E., Lacy, A. R., & Reddy, M. C. (1998). Functional analysis of copper homeostasis in cell culture models: a new perspective on internal copper transport. The American Journal of Clinical Nutrition, 67(5 Suppl), 988S-995S. https://doi.org/10.1093/ajcn/67.5.988S
Harris ED, et al. Functional Analysis of Copper Homeostasis in Cell Culture Models: a New Perspective On Internal Copper Transport. Am J Clin Nutr. 1998;67(5 Suppl):988S-995S. PubMed PMID: 9587141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional analysis of copper homeostasis in cell culture models: a new perspective on internal copper transport. AU - Harris,E D, AU - Qian,Y, AU - Tiffany-Castiglioni,E, AU - Lacy,A R, AU - Reddy,M C, PY - 1998/5/20/pubmed PY - 1998/5/20/medline PY - 1998/5/20/entrez SP - 988S EP - 995S JF - The American journal of clinical nutrition JO - Am. J. Clin. Nutr. VL - 67 IS - 5 Suppl N2 - The movement of copper ions across membrane barriers of vital organs and tissues is a priority topic in nutrition and one for which there continues to be little understanding of the mechanism. Reports of membrane-bound, copper-transporting adenosine triphosphatases (Cu-ATPases) selective for copper ions have brought new focus to the problem and prompted fresh ideas. Using a cell culture model approach, we attempted to learn whether transport into and out of cells depends on a Cu-ATPase. Measurement of transport kinetics in fibroblasts, brain glial cells, neuroblastoma cells, and placental cells showed differences in the rates of copper uptake and response to sulfhydryl reagents. BeWo cells, a human choriocarcinoma placental cell line, behaved as did Menkes fibroblasts by avidly absorbing copper but not releasing copper to the immediate environment. Further tests showed that BeWo cells did not express the transcript for the membrane-bound Cu-ATPase that has been identified with Menkes syndrome. Transcript induction, however, was achieved by growing BeWo cells on porous filters that allowed apical and basolateral surfaces to form. With transcript expression, the cells showed a capacity to release copper into the medium. BeWo cells also synthesized a form of ceruloplasmin whose structure differed from that of the plasma protein and hence may be a product of a different gene. BeWo cells may also express the gene for Wilson disease, thus linking Menkes and Wilson proteins to maternal delivery of copper. We constructed a model in which both ATPases work in concert in a vesicle-based transport mechanism. The vesicle model may help us understand the transport of copper across the placenta and all cells in general. SN - 0002-9165 UR - https://www.unboundmedicine.com/medline/citation/9587141/Functional_analysis_of_copper_homeostasis_in_cell_culture_models:_a_new_perspective_on_internal_copper_transport_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.1093/ajcn/67.5.988S DB - PRIME DP - Unbound Medicine ER -