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Growth characteristics and metastatic properties of human breast cancer xenografts in immunodeficient mice.
Am J Pathol. 1998 May; 152(5):1299-311.AJ

Abstract

We evaluated the growth and metastatic potential of two human breast cancer cell lines and 16 patient-derived biopsy specimens, representing the most common histological types of breast carcinomas, upon subcutaneous implantation into severe combined immunodeficient (SCID) mice. The method of engraftment we used, based on implantation of intact tissue specimens and complete immunosuppression of the host, provided an easier system to grow human breast carcinoma specimens in mouse models and resulted in a 50% success rate of tumor take. No correlation was found between growth in SCID mice and pathological diagnosis, grading, or estrogen/progesterone receptor expression by the tumor biopsy specimen. Serial passage of the tumor fragments in SCID mice resulted in increased metastasis rates and more rapid emergence of a palpable tumor mass. A tumor from a patient with infiltrating ductal carcinoma, which grew aggressively and metastasized in 100% of the female SCID mice, was also successfully engrafted in 100% of nonobese diabetic (NOD)/SCID female mice, but systemic spread was minimal. Fragments of the same tumor grew in only 33% of male SCID mice with very limited metastases. A strong correlation (r = 0.997) was observed between tumor burden and the presence of soluble (serum) interleukin-2 receptor, a marker associated with a subset of human breast tumors. All together, these data indicate the usefulness of SCID/human breast tumor xenografts for measuring tumor progression and evaluating novel therapeutic approaches to breast cancer.

Authors+Show Affiliations

The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9588898

Citation

Visonneau, S, et al. "Growth Characteristics and Metastatic Properties of Human Breast Cancer Xenografts in Immunodeficient Mice." The American Journal of Pathology, vol. 152, no. 5, 1998, pp. 1299-311.
Visonneau S, Cesano A, Torosian MH, et al. Growth characteristics and metastatic properties of human breast cancer xenografts in immunodeficient mice. Am J Pathol. 1998;152(5):1299-311.
Visonneau, S., Cesano, A., Torosian, M. H., Miller, E. J., & Santoli, D. (1998). Growth characteristics and metastatic properties of human breast cancer xenografts in immunodeficient mice. The American Journal of Pathology, 152(5), 1299-311.
Visonneau S, et al. Growth Characteristics and Metastatic Properties of Human Breast Cancer Xenografts in Immunodeficient Mice. Am J Pathol. 1998;152(5):1299-311. PubMed PMID: 9588898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Growth characteristics and metastatic properties of human breast cancer xenografts in immunodeficient mice. AU - Visonneau,S, AU - Cesano,A, AU - Torosian,M H, AU - Miller,E J, AU - Santoli,D, PY - 1998/5/20/pubmed PY - 1998/5/20/medline PY - 1998/5/20/entrez SP - 1299 EP - 311 JF - The American journal of pathology JO - Am J Pathol VL - 152 IS - 5 N2 - We evaluated the growth and metastatic potential of two human breast cancer cell lines and 16 patient-derived biopsy specimens, representing the most common histological types of breast carcinomas, upon subcutaneous implantation into severe combined immunodeficient (SCID) mice. The method of engraftment we used, based on implantation of intact tissue specimens and complete immunosuppression of the host, provided an easier system to grow human breast carcinoma specimens in mouse models and resulted in a 50% success rate of tumor take. No correlation was found between growth in SCID mice and pathological diagnosis, grading, or estrogen/progesterone receptor expression by the tumor biopsy specimen. Serial passage of the tumor fragments in SCID mice resulted in increased metastasis rates and more rapid emergence of a palpable tumor mass. A tumor from a patient with infiltrating ductal carcinoma, which grew aggressively and metastasized in 100% of the female SCID mice, was also successfully engrafted in 100% of nonobese diabetic (NOD)/SCID female mice, but systemic spread was minimal. Fragments of the same tumor grew in only 33% of male SCID mice with very limited metastases. A strong correlation (r = 0.997) was observed between tumor burden and the presence of soluble (serum) interleukin-2 receptor, a marker associated with a subset of human breast tumors. All together, these data indicate the usefulness of SCID/human breast tumor xenografts for measuring tumor progression and evaluating novel therapeutic approaches to breast cancer. SN - 0002-9440 UR - https://www.unboundmedicine.com/medline/citation/9588898/Growth_characteristics_and_metastatic_properties_of_human_breast_cancer_xenografts_in_immunodeficient_mice_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/9588898/ DB - PRIME DP - Unbound Medicine ER -