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In situ immune response in gut-associated lymphoid tissue (GALT) following oral antigen in TCR-transgenic mice.
J Immunol. 1998 May 15; 160(10):4708-18.JI

Abstract

Oral administration of Ag results in systemic hyporesponsiveness termed oral tolerance. The regulatory cells induced by oral Ag are effective in the suppression of Th1-type autoimmune diseases. We examined the cytokine microenvironment in gut-associated lymphoid tissue in response to orally administered OVA in OVA TCR-transgenic mice. Mice were fed a low (0.5 mg) or high (500 mg) dose of OVA one time or five times. Immunohistochemical analysis demonstrated increased IL-4, IL-10, and TGF-beta in the gut within 6 h of a low-dose feeding and after five low-dose or high-dose feedings. IFN-gamma and IL-2 either decreased or showed no change, with the exception of a small transient increase in IL-2 at 6 h after a low dose. Increases in IL-4 and IL-10 were found in the dome of the Peyer's patch, and increases in TGF-beta were observed in the interfollicular region and the villi. IL-10 was also substantially increased in the villi. IL-4 and IL-10 were produced predominately by CD4+ T cells. TGF-beta was found predominately in macrophages and CD4+ T cells. Peyer's patches had a marked up-regulation of TGF-beta mRNA as measured by RT-PCR. These results demonstrate the differential activation of cytokine production in discrete regions of gut-associated lymphoid tissue. The induction of cytokines known to inhibit autoimmune disease at the site of Ag absorption indicates an important role for the mucosal immune system in the establishment of oral tolerance.

Authors+Show Affiliations

Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA. gonnella@cnd.bwh.harvard.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9590216

Citation

Gonnella, P A., et al. "In Situ Immune Response in Gut-associated Lymphoid Tissue (GALT) Following Oral Antigen in TCR-transgenic Mice." Journal of Immunology (Baltimore, Md. : 1950), vol. 160, no. 10, 1998, pp. 4708-18.
Gonnella PA, Chen Y, Inobe J, et al. In situ immune response in gut-associated lymphoid tissue (GALT) following oral antigen in TCR-transgenic mice. J Immunol. 1998;160(10):4708-18.
Gonnella, P. A., Chen, Y., Inobe, J., Komagata, Y., Quartulli, M., & Weiner, H. L. (1998). In situ immune response in gut-associated lymphoid tissue (GALT) following oral antigen in TCR-transgenic mice. Journal of Immunology (Baltimore, Md. : 1950), 160(10), 4708-18.
Gonnella PA, et al. In Situ Immune Response in Gut-associated Lymphoid Tissue (GALT) Following Oral Antigen in TCR-transgenic Mice. J Immunol. 1998 May 15;160(10):4708-18. PubMed PMID: 9590216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In situ immune response in gut-associated lymphoid tissue (GALT) following oral antigen in TCR-transgenic mice. AU - Gonnella,P A, AU - Chen,Y, AU - Inobe,J, AU - Komagata,Y, AU - Quartulli,M, AU - Weiner,H L, PY - 1998/5/20/pubmed PY - 1998/5/20/medline PY - 1998/5/20/entrez SP - 4708 EP - 18 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 160 IS - 10 N2 - Oral administration of Ag results in systemic hyporesponsiveness termed oral tolerance. The regulatory cells induced by oral Ag are effective in the suppression of Th1-type autoimmune diseases. We examined the cytokine microenvironment in gut-associated lymphoid tissue in response to orally administered OVA in OVA TCR-transgenic mice. Mice were fed a low (0.5 mg) or high (500 mg) dose of OVA one time or five times. Immunohistochemical analysis demonstrated increased IL-4, IL-10, and TGF-beta in the gut within 6 h of a low-dose feeding and after five low-dose or high-dose feedings. IFN-gamma and IL-2 either decreased or showed no change, with the exception of a small transient increase in IL-2 at 6 h after a low dose. Increases in IL-4 and IL-10 were found in the dome of the Peyer's patch, and increases in TGF-beta were observed in the interfollicular region and the villi. IL-10 was also substantially increased in the villi. IL-4 and IL-10 were produced predominately by CD4+ T cells. TGF-beta was found predominately in macrophages and CD4+ T cells. Peyer's patches had a marked up-regulation of TGF-beta mRNA as measured by RT-PCR. These results demonstrate the differential activation of cytokine production in discrete regions of gut-associated lymphoid tissue. The induction of cytokines known to inhibit autoimmune disease at the site of Ag absorption indicates an important role for the mucosal immune system in the establishment of oral tolerance. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/9590216/In_situ_immune_response_in_gut_associated_lymphoid_tissue__GALT__following_oral_antigen_in_TCR_transgenic_mice_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=9590216 DB - PRIME DP - Unbound Medicine ER -