Tags

Type your tag names separated by a space and hit enter

Expression of pro-inflammatory cytokines by flow-sorted alveolar macrophages in severe pneumonia.
Eur Respir J. 1998 Mar; 11(3):534-41.ER

Abstract

The aim of the present study was to further characterize the role of alveolar macrophages (AM) in acute human lung inflammation by evaluating their capacity to produce pro-inflammatory cytokines such as tumour necrosis factor (TNF)-alpha, interleukin (IL)-6 and IL-8. Patients with severe community-acquired pneumonia (CAP; n=12) and healthy volunteers (n=10) underwent bronchoalveolar lavage (BAL). AM were separated to high purity (>96%) using fluorescence-activated cell sorting. We determined the TNF-alpha, IL-6 and IL-8 cytokine gene expression in AM ex vivo using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). Moreover, we measured in vitro unstimulated, lipopolysaccharide (LPS)- and LPS/interferon-gamma inducible TNF-alpha, IL-6 and IL-8 cytokine release and evaluated samples of BAL fluids for the same pro-inflammatory cytokines using an enzyme-linked immunosorbent assay (ELISA). We found increased TNF-alpha, IL-6 and IL-8 messenger ribonucleic acid (mRNA) levels in AM from CAP patients that were significantly elevated only for IL-8. When challenged with endotoxin in vitro, AM obtained from CAP patients showed a strongly reduced potential to release TNF-alpha and IL-6 compared to healthy controls, whereas IL-8 secretion did not differ significantly between groups. Moreover, stimulation of AM from CAP patients with LPS plus IFN-gamma augmented TNF-alpha and IL-6 cytokine release to near normal levels. Interestingly, no TNF-alpha protein was measured in BAL samples from CAP patients, whereas IL-6 and IL-8 protein levels were found to be significantly increased. Together, highly purified alveolar macrophages from community-acquired pneumonia patients show relatively low ex vivo tumour necrosis factor-alpha and interleukin-6 but not interleukin-8 messenger ribonucleic acid levels that are associated with a decreased pro-inflammatory cytokine release in vitro which, however, can be restored by concurrent interferon-gamma stimulation.

Authors+Show Affiliations

Dept of Internal Medicine, Justus-Liebig-University, Giessen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9596098

Citation

Maus, U, et al. "Expression of Pro-inflammatory Cytokines By Flow-sorted Alveolar Macrophages in Severe Pneumonia." The European Respiratory Journal, vol. 11, no. 3, 1998, pp. 534-41.
Maus U, Rosseau S, Knies U, et al. Expression of pro-inflammatory cytokines by flow-sorted alveolar macrophages in severe pneumonia. Eur Respir J. 1998;11(3):534-41.
Maus, U., Rosseau, S., Knies, U., Seeger, W., & Lohmeyer, J. (1998). Expression of pro-inflammatory cytokines by flow-sorted alveolar macrophages in severe pneumonia. The European Respiratory Journal, 11(3), 534-41.
Maus U, et al. Expression of Pro-inflammatory Cytokines By Flow-sorted Alveolar Macrophages in Severe Pneumonia. Eur Respir J. 1998;11(3):534-41. PubMed PMID: 9596098.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of pro-inflammatory cytokines by flow-sorted alveolar macrophages in severe pneumonia. AU - Maus,U, AU - Rosseau,S, AU - Knies,U, AU - Seeger,W, AU - Lohmeyer,J, PY - 1998/5/22/pubmed PY - 1998/5/22/medline PY - 1998/5/22/entrez SP - 534 EP - 41 JF - The European respiratory journal JO - Eur Respir J VL - 11 IS - 3 N2 - The aim of the present study was to further characterize the role of alveolar macrophages (AM) in acute human lung inflammation by evaluating their capacity to produce pro-inflammatory cytokines such as tumour necrosis factor (TNF)-alpha, interleukin (IL)-6 and IL-8. Patients with severe community-acquired pneumonia (CAP; n=12) and healthy volunteers (n=10) underwent bronchoalveolar lavage (BAL). AM were separated to high purity (>96%) using fluorescence-activated cell sorting. We determined the TNF-alpha, IL-6 and IL-8 cytokine gene expression in AM ex vivo using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). Moreover, we measured in vitro unstimulated, lipopolysaccharide (LPS)- and LPS/interferon-gamma inducible TNF-alpha, IL-6 and IL-8 cytokine release and evaluated samples of BAL fluids for the same pro-inflammatory cytokines using an enzyme-linked immunosorbent assay (ELISA). We found increased TNF-alpha, IL-6 and IL-8 messenger ribonucleic acid (mRNA) levels in AM from CAP patients that were significantly elevated only for IL-8. When challenged with endotoxin in vitro, AM obtained from CAP patients showed a strongly reduced potential to release TNF-alpha and IL-6 compared to healthy controls, whereas IL-8 secretion did not differ significantly between groups. Moreover, stimulation of AM from CAP patients with LPS plus IFN-gamma augmented TNF-alpha and IL-6 cytokine release to near normal levels. Interestingly, no TNF-alpha protein was measured in BAL samples from CAP patients, whereas IL-6 and IL-8 protein levels were found to be significantly increased. Together, highly purified alveolar macrophages from community-acquired pneumonia patients show relatively low ex vivo tumour necrosis factor-alpha and interleukin-6 but not interleukin-8 messenger ribonucleic acid levels that are associated with a decreased pro-inflammatory cytokine release in vitro which, however, can be restored by concurrent interferon-gamma stimulation. SN - 0903-1936 UR - https://www.unboundmedicine.com/medline/citation/9596098/Expression_of_pro_inflammatory_cytokines_by_flow_sorted_alveolar_macrophages_in_severe_pneumonia_ L2 - http://erj.ersjournals.com/cgi/pmidlookup?view=long&pmid=9596098 DB - PRIME DP - Unbound Medicine ER -