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Multiple fimbrial adhesins are required for full virulence of Salmonella typhimurium in mice.
Infect Immun. 1998 Jun; 66(6):2803-8.II

Abstract

Adhesion is an important initial step during bacterial colonization of the intestinal mucosa. However, mutations in the Salmonella typhimurium fimbrial operons lpf, pef, or fim only moderately alter mouse virulence. The respective adhesins may thus play only a minor role during infection or S. typhimurium may encode alternative virulence factors that can functionally compensate for their loss. To address this question, we constructed mutations in all four known fimbrial operons of S. typhimurium: fim, lpf, pef, and agf. A mutation in the agfB gene resulted in a threefold increase in the oral 50% lethal dose (LD50) of S. typhimurium for mice. In contrast, an S. typhimurium strain carrying mutations in all four fimbrial operons (quadruple mutant) had a 26-fold increased oral LD50. The quadruple mutant, but not the agfB mutant, was recovered in reduced numbers from murine fecal pellets, suggesting that a reduced ability to colonize the intestinal lumen contributed to its attenuation. These data are evidence for a synergistic action of fimbrial operons during colonization of the mouse intestine and the development of murine typhoid fever.

Authors+Show Affiliations

Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9596751

Citation

van der Velden, A W., et al. "Multiple Fimbrial Adhesins Are Required for Full Virulence of Salmonella Typhimurium in Mice." Infection and Immunity, vol. 66, no. 6, 1998, pp. 2803-8.
van der Velden AW, Bäumler AJ, Tsolis RM, et al. Multiple fimbrial adhesins are required for full virulence of Salmonella typhimurium in mice. Infect Immun. 1998;66(6):2803-8.
van der Velden, A. W., Bäumler, A. J., Tsolis, R. M., & Heffron, F. (1998). Multiple fimbrial adhesins are required for full virulence of Salmonella typhimurium in mice. Infection and Immunity, 66(6), 2803-8.
van der Velden AW, et al. Multiple Fimbrial Adhesins Are Required for Full Virulence of Salmonella Typhimurium in Mice. Infect Immun. 1998;66(6):2803-8. PubMed PMID: 9596751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple fimbrial adhesins are required for full virulence of Salmonella typhimurium in mice. AU - van der Velden,A W, AU - Bäumler,A J, AU - Tsolis,R M, AU - Heffron,F, PY - 1998/5/29/pubmed PY - 1998/5/29/medline PY - 1998/5/29/entrez SP - 2803 EP - 8 JF - Infection and immunity JO - Infect Immun VL - 66 IS - 6 N2 - Adhesion is an important initial step during bacterial colonization of the intestinal mucosa. However, mutations in the Salmonella typhimurium fimbrial operons lpf, pef, or fim only moderately alter mouse virulence. The respective adhesins may thus play only a minor role during infection or S. typhimurium may encode alternative virulence factors that can functionally compensate for their loss. To address this question, we constructed mutations in all four known fimbrial operons of S. typhimurium: fim, lpf, pef, and agf. A mutation in the agfB gene resulted in a threefold increase in the oral 50% lethal dose (LD50) of S. typhimurium for mice. In contrast, an S. typhimurium strain carrying mutations in all four fimbrial operons (quadruple mutant) had a 26-fold increased oral LD50. The quadruple mutant, but not the agfB mutant, was recovered in reduced numbers from murine fecal pellets, suggesting that a reduced ability to colonize the intestinal lumen contributed to its attenuation. These data are evidence for a synergistic action of fimbrial operons during colonization of the mouse intestine and the development of murine typhoid fever. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/9596751/Multiple_fimbrial_adhesins_are_required_for_full_virulence_of_Salmonella_typhimurium_in_mice_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=9596751 DB - PRIME DP - Unbound Medicine ER -