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IL-10-mediated suppression of TNF-alpha production is independent of its ability to inhibit NF kappa B activity.
Eur J Immunol. 1998 May; 28(5):1719-26.EJ

Abstract

IL-10 has a well-characterized anti-inflammatory role that includes the suppression of inflammatory cytokine (e.g. TNF-alpha) production by monocytic/macrophage cells. Both transcriptional and post-transcriptional/translational mechanisms have been proposed to explain this process. In this study we observed that IL-10 inhibited nuclear NF kappa B DNA binding activity without affecting I kappa B degradation or translocation of NF kappa B subunits to the nucleus. While the suppression of NF kappa B in 70Z/3 pre-B cells correlated with suppression of NF kappa B transcriptional activity and expression of surface IgM, it did not correlate with the production of TNF-alpha mRNA or protein in RAW 264.7 macrophages. Similar observations in the macrophages were made with a second anti-inflammatory cytokine, IL-4. Therefore we conclude that although IL-10 or IL-4 can suppress NF kappa B activity, this appears to have little effect on the expression of the TNF-alpha gene and is unlikely to be the basis of the anti-inflammatory effects of these cytokines.

Authors+Show Affiliations

Kennedy Institute of Rheumatology, Hammersmith, London, GB.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9603479

Citation

Clarke, C J., et al. "IL-10-mediated Suppression of TNF-alpha Production Is Independent of Its Ability to Inhibit NF Kappa B Activity." European Journal of Immunology, vol. 28, no. 5, 1998, pp. 1719-26.
Clarke CJ, Hales A, Hunt A, et al. IL-10-mediated suppression of TNF-alpha production is independent of its ability to inhibit NF kappa B activity. Eur J Immunol. 1998;28(5):1719-26.
Clarke, C. J., Hales, A., Hunt, A., & Foxwell, B. M. (1998). IL-10-mediated suppression of TNF-alpha production is independent of its ability to inhibit NF kappa B activity. European Journal of Immunology, 28(5), 1719-26.
Clarke CJ, et al. IL-10-mediated Suppression of TNF-alpha Production Is Independent of Its Ability to Inhibit NF Kappa B Activity. Eur J Immunol. 1998;28(5):1719-26. PubMed PMID: 9603479.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-10-mediated suppression of TNF-alpha production is independent of its ability to inhibit NF kappa B activity. AU - Clarke,C J, AU - Hales,A, AU - Hunt,A, AU - Foxwell,B M, PY - 1998/5/29/pubmed PY - 2000/6/20/medline PY - 1998/5/29/entrez SP - 1719 EP - 26 JF - European journal of immunology JO - Eur J Immunol VL - 28 IS - 5 N2 - IL-10 has a well-characterized anti-inflammatory role that includes the suppression of inflammatory cytokine (e.g. TNF-alpha) production by monocytic/macrophage cells. Both transcriptional and post-transcriptional/translational mechanisms have been proposed to explain this process. In this study we observed that IL-10 inhibited nuclear NF kappa B DNA binding activity without affecting I kappa B degradation or translocation of NF kappa B subunits to the nucleus. While the suppression of NF kappa B in 70Z/3 pre-B cells correlated with suppression of NF kappa B transcriptional activity and expression of surface IgM, it did not correlate with the production of TNF-alpha mRNA or protein in RAW 264.7 macrophages. Similar observations in the macrophages were made with a second anti-inflammatory cytokine, IL-4. Therefore we conclude that although IL-10 or IL-4 can suppress NF kappa B activity, this appears to have little effect on the expression of the TNF-alpha gene and is unlikely to be the basis of the anti-inflammatory effects of these cytokines. SN - 0014-2980 UR - https://www.unboundmedicine.com/medline/citation/9603479/IL_10_mediated_suppression_of_TNF_alpha_production_is_independent_of_its_ability_to_inhibit_NF_kappa_B_activity_ L2 - https://doi.org/10.1002/(SICI)1521-4141(199805)28:05<1719::AID-IMMU1719>3.0.CO;2-Q DB - PRIME DP - Unbound Medicine ER -