Tags

Type your tag names separated by a space and hit enter

No significant role of Epstein-Barr virus in the tumorigenesis of Warthin tumor.
J Med Dent Sci. 1997 Jun; 44(2):45-52.JM

Abstract

To determine the significance of Epstein-Barr virus (EBV) in the tumorigenesis of Warthin tumor, formaldehyde-fixed and paraffin-embedded specimens of 21 tumors from 18 patients were examined by polymerase chain reaction (PCR)-Southern hybridization, in situ hybridization (ISH), and immunohistochemistry. PCR-Southern hybridization revealed the presence of EBV DNA in 13 of the 21 tumors (61.9%). However, ISH for EBV RNA showed that nuclei of the neoplastic epithelial cells of all tumors were negative. Although ISH for EBV DNA revealed that the nuclei of the neoplastic epithelial cells were positive in 4 of the 21 tumors (19.0%), the positive cells were sparsely distributed and there was no evidence of monoclonal proliferation of EBV positive neoplastic epithelial cells. Moreover, immunohistochemical reactions with antibodies against latent membrane protein 1 (LMP1) and EBV nuclear antigen 2 (EBNA2), were negative in all cases. Judging from these findings, we conclude that EBV does not play a major role in the tumorigenesis of Warthin tumor.

Authors+Show Affiliations

Department of Oral Pathology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

9608281

Citation

Ogata, T, et al. "No Significant Role of Epstein-Barr Virus in the Tumorigenesis of Warthin Tumor." Journal of Medical and Dental Sciences, vol. 44, no. 2, 1997, pp. 45-52.
Ogata T, Hongfang Y, Kayano T, et al. No significant role of Epstein-Barr virus in the tumorigenesis of Warthin tumor. J Med Dent Sci. 1997;44(2):45-52.
Ogata, T., Hongfang, Y., Kayano, T., & Hirai, K. (1997). No significant role of Epstein-Barr virus in the tumorigenesis of Warthin tumor. Journal of Medical and Dental Sciences, 44(2), 45-52.
Ogata T, et al. No Significant Role of Epstein-Barr Virus in the Tumorigenesis of Warthin Tumor. J Med Dent Sci. 1997;44(2):45-52. PubMed PMID: 9608281.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - No significant role of Epstein-Barr virus in the tumorigenesis of Warthin tumor. AU - Ogata,T, AU - Hongfang,Y, AU - Kayano,T, AU - Hirai,K, PY - 1997/6/1/pubmed PY - 1998/6/3/medline PY - 1997/6/1/entrez SP - 45 EP - 52 JF - Journal of medical and dental sciences JO - J. Med. Dent. Sci. VL - 44 IS - 2 N2 - To determine the significance of Epstein-Barr virus (EBV) in the tumorigenesis of Warthin tumor, formaldehyde-fixed and paraffin-embedded specimens of 21 tumors from 18 patients were examined by polymerase chain reaction (PCR)-Southern hybridization, in situ hybridization (ISH), and immunohistochemistry. PCR-Southern hybridization revealed the presence of EBV DNA in 13 of the 21 tumors (61.9%). However, ISH for EBV RNA showed that nuclei of the neoplastic epithelial cells of all tumors were negative. Although ISH for EBV DNA revealed that the nuclei of the neoplastic epithelial cells were positive in 4 of the 21 tumors (19.0%), the positive cells were sparsely distributed and there was no evidence of monoclonal proliferation of EBV positive neoplastic epithelial cells. Moreover, immunohistochemical reactions with antibodies against latent membrane protein 1 (LMP1) and EBV nuclear antigen 2 (EBNA2), were negative in all cases. Judging from these findings, we conclude that EBV does not play a major role in the tumorigenesis of Warthin tumor. SN - 1342-8810 UR - https://www.unboundmedicine.com/medline/citation/9608281/No_significant_role_of_Epstein_Barr_virus_in_the_tumorigenesis_of_Warthin_tumor_ DB - PRIME DP - Unbound Medicine ER -