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Effect of serotonin on ouabain-sensitive, K+-dependent, p-nitrophenylphosphatase activity in strial marginal cells of normal and reserpinized guinea pigs.
Histochem J. 1998 Apr; 30(4):263-6.HJ

Abstract

Na+,K+-ATPase activity is abundant on the basolateral infoldings of the strial marginal cells and contributes to the maintenance of the characteristic electrolyte composition of the endolymph. However, the stria vascularis of the cochlea is known not to be innervated. In order to clarify its humoral regulation by serotonin, the K+-p-nitrophenylphosphatase activity of strial marginal cells was investigated with a cerium-based method in normal guinea pigs and in guinea pigs treated with reserpine, 5-hydroxytryptamine or reserpine plus 5-hydroxytryptamine. K+-p-nitrophenylphosphatase activity was almost completely depressed 3-20 days after reserpine administration. Ten days after reserpinization, followed by repeated 5-hydroxytryptamine treatment, the enzyme activity was detectable. These results suggest that 5-hydroxytryptamine increases the phosphatase activity. Thus, the function of the stria vascularis in producing cochlear endolymph may be regulated by 5-hydroxytryptamine.

Authors+Show Affiliations

Department of Otolaryngology, Hyogo College of Medicine, Nishinomiya, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9610817

Citation

Kanoh, N, et al. "Effect of Serotonin On Ouabain-sensitive, K+-dependent, P-nitrophenylphosphatase Activity in Strial Marginal Cells of Normal and Reserpinized Guinea Pigs." The Histochemical Journal, vol. 30, no. 4, 1998, pp. 263-6.
Kanoh N, Hori K, Ishigaki T, et al. Effect of serotonin on ouabain-sensitive, K+-dependent, p-nitrophenylphosphatase activity in strial marginal cells of normal and reserpinized guinea pigs. Histochem J. 1998;30(4):263-6.
Kanoh, N., Hori, K., Ishigaki, T., & Hori, S. (1998). Effect of serotonin on ouabain-sensitive, K+-dependent, p-nitrophenylphosphatase activity in strial marginal cells of normal and reserpinized guinea pigs. The Histochemical Journal, 30(4), 263-6.
Kanoh N, et al. Effect of Serotonin On Ouabain-sensitive, K+-dependent, P-nitrophenylphosphatase Activity in Strial Marginal Cells of Normal and Reserpinized Guinea Pigs. Histochem J. 1998;30(4):263-6. PubMed PMID: 9610817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of serotonin on ouabain-sensitive, K+-dependent, p-nitrophenylphosphatase activity in strial marginal cells of normal and reserpinized guinea pigs. AU - Kanoh,N, AU - Hori,K, AU - Ishigaki,T, AU - Hori,S, PY - 1998/6/4/pubmed PY - 1998/6/4/medline PY - 1998/6/4/entrez SP - 263 EP - 6 JF - The Histochemical journal JO - Histochem J VL - 30 IS - 4 N2 - Na+,K+-ATPase activity is abundant on the basolateral infoldings of the strial marginal cells and contributes to the maintenance of the characteristic electrolyte composition of the endolymph. However, the stria vascularis of the cochlea is known not to be innervated. In order to clarify its humoral regulation by serotonin, the K+-p-nitrophenylphosphatase activity of strial marginal cells was investigated with a cerium-based method in normal guinea pigs and in guinea pigs treated with reserpine, 5-hydroxytryptamine or reserpine plus 5-hydroxytryptamine. K+-p-nitrophenylphosphatase activity was almost completely depressed 3-20 days after reserpine administration. Ten days after reserpinization, followed by repeated 5-hydroxytryptamine treatment, the enzyme activity was detectable. These results suggest that 5-hydroxytryptamine increases the phosphatase activity. Thus, the function of the stria vascularis in producing cochlear endolymph may be regulated by 5-hydroxytryptamine. SN - 0018-2214 UR - https://www.unboundmedicine.com/medline/citation/9610817/Effect_of_serotonin_on_ouabain_sensitive_K+_dependent_p_nitrophenylphosphatase_activity_in_strial_marginal_cells_of_normal_and_reserpinized_guinea_pigs_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=9610817.ui DB - PRIME DP - Unbound Medicine ER -