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Potential retroviruses in plants: Tat1 is related to a group of Arabidopsis thaliana Ty3/gypsy retrotransposons that encode envelope-like proteins.
Genetics 1998; 149(2):703-15G

Abstract

Tat1 was originally identified as an insertion near the Arabidopsis thaliana SAM1 gene. We provide evidence that Tat1 is a retrotransposon and that previously described insertions are solo long terminal repeats (LTRs) left behind after the deletion of coding regions of full-length elements. Three Tat1 insertions were characterized that have retrotransposon features, including a primer binding site complementary to an A. thaliana asparagine tRNA and an open reading frame (ORF) with approximately 44% amino acid sequence similarity to the gag protein of the Zea mays retrotransposon Zeon-1. Tat1 elements have large, polymorphic 3' noncoding regions that may contain transduced DNA sequences; a 477-base insertion in the 3' noncoding region of the Tat1-3 element contains part of a related retrotransposon and sequences similar to the nontranslated leader sequence of AT-P5C1, a gene for pyrroline-5-carboxylate reductase. Analysis of DNA sequences generated by the A. thaliana genome project identified 10 families of Ty3/gypsy retrotransposons, which share up to 51 and 62% amino-acid similarity to the ORFs of Tat1 and the A. thaliana Athila element, respectively. Phylogenetic analyses resolved the plant Ty3/gypsy elements into two lineages, one of which includes homologs of Tat1 and Athila. Four families of A. thaliana elements within the Tat/Athila lineage encode a conserved ORF after integrase at a position occupied by the envelope gene in retroviruses and in some insect Ty3/gypsy retrotransposons. Like retroviral envelope genes, this ORF encodes a transmembrane domain and, in some insertions, a putative secretory signal sequence. This suggests that Tat/Athila retrotransposons may produce enveloped virions and may be infectious.

Authors+Show Affiliations

Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9611185

Citation

Wright, D A., and D F. Voytas. "Potential Retroviruses in Plants: Tat1 Is Related to a Group of Arabidopsis Thaliana Ty3/gypsy Retrotransposons That Encode Envelope-like Proteins." Genetics, vol. 149, no. 2, 1998, pp. 703-15.
Wright DA, Voytas DF. Potential retroviruses in plants: Tat1 is related to a group of Arabidopsis thaliana Ty3/gypsy retrotransposons that encode envelope-like proteins. Genetics. 1998;149(2):703-15.
Wright, D. A., & Voytas, D. F. (1998). Potential retroviruses in plants: Tat1 is related to a group of Arabidopsis thaliana Ty3/gypsy retrotransposons that encode envelope-like proteins. Genetics, 149(2), pp. 703-15.
Wright DA, Voytas DF. Potential Retroviruses in Plants: Tat1 Is Related to a Group of Arabidopsis Thaliana Ty3/gypsy Retrotransposons That Encode Envelope-like Proteins. Genetics. 1998;149(2):703-15. PubMed PMID: 9611185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential retroviruses in plants: Tat1 is related to a group of Arabidopsis thaliana Ty3/gypsy retrotransposons that encode envelope-like proteins. AU - Wright,D A, AU - Voytas,D F, PY - 1998/6/11/pubmed PY - 1998/6/11/medline PY - 1998/6/11/entrez SP - 703 EP - 15 JF - Genetics JO - Genetics VL - 149 IS - 2 N2 - Tat1 was originally identified as an insertion near the Arabidopsis thaliana SAM1 gene. We provide evidence that Tat1 is a retrotransposon and that previously described insertions are solo long terminal repeats (LTRs) left behind after the deletion of coding regions of full-length elements. Three Tat1 insertions were characterized that have retrotransposon features, including a primer binding site complementary to an A. thaliana asparagine tRNA and an open reading frame (ORF) with approximately 44% amino acid sequence similarity to the gag protein of the Zea mays retrotransposon Zeon-1. Tat1 elements have large, polymorphic 3' noncoding regions that may contain transduced DNA sequences; a 477-base insertion in the 3' noncoding region of the Tat1-3 element contains part of a related retrotransposon and sequences similar to the nontranslated leader sequence of AT-P5C1, a gene for pyrroline-5-carboxylate reductase. Analysis of DNA sequences generated by the A. thaliana genome project identified 10 families of Ty3/gypsy retrotransposons, which share up to 51 and 62% amino-acid similarity to the ORFs of Tat1 and the A. thaliana Athila element, respectively. Phylogenetic analyses resolved the plant Ty3/gypsy elements into two lineages, one of which includes homologs of Tat1 and Athila. Four families of A. thaliana elements within the Tat/Athila lineage encode a conserved ORF after integrase at a position occupied by the envelope gene in retroviruses and in some insect Ty3/gypsy retrotransposons. Like retroviral envelope genes, this ORF encodes a transmembrane domain and, in some insertions, a putative secretory signal sequence. This suggests that Tat/Athila retrotransposons may produce enveloped virions and may be infectious. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/9611185/Potential_retroviruses_in_plants:_Tat1_is_related_to_a_group_of_Arabidopsis_thaliana_Ty3/gypsy_retrotransposons_that_encode_envelope_like_proteins_ L2 - http://www.genetics.org/cgi/pmidlookup?view=long&pmid=9611185 DB - PRIME DP - Unbound Medicine ER -