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Expression of HNF-1 alpha and HNF-1 beta in various histological differentiations of hepatocellular carcinoma.
J Pathol. 1998 Mar; 184(3):272-8.JP

Abstract

Hepatic nuclear factor 1 (HNF-1) regulates genes in a hepatocyte-specific manner. It has been previously reported that the ratio of HNF-1 alpha and HNF-1 beta mRNA is related to histological differentiation hepatocellular carcinoma (HCC). In this study, the expression levels of the HNF-1 alpha and HNF-1 beta proteins were analysed relatively and quantitatively in various histologically differentiated HCC and surrounding non-cancerous tissues, and HNF-1 alpha binding activity for the AT element of the B domain of the human alpha-fetoprotein enhancer was examined. Western blot analysis demonstrated that HNF-1 alpha protein was expressed at a higher level in well-differentiated HCC tissues than in the surrounding non-HCC tissues; on the other hand, the HNF-1 alpha protein was expressed at lower levels in moderately and poorly differentiated HCCs than in the surrounding non-HCC tissues. The levels of HNF-1 beta expression in well-differentiated and poorly differentiated HCCs were similar to and higher than those found in the respective surrounding non-cancerous portions. In binding assays, HNF-1 binding activity was high in well-differentiated HCC and lower in moderately and poorly differentiated HCCs. Most well-differentiated HCC cases showed immunohistochemical expression of HNF-1 alpha. These findings show that poor histological differentiation of HCC correlates with decreases in the level and activity of HNF-1 alpha proteins.

Authors+Show Affiliations

First Division of Pathology, Kobe University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9614379

Citation

Wang, W, et al. "Expression of HNF-1 Alpha and HNF-1 Beta in Various Histological Differentiations of Hepatocellular Carcinoma." The Journal of Pathology, vol. 184, no. 3, 1998, pp. 272-8.
Wang W, Hayashi Y, Ninomiya T, et al. Expression of HNF-1 alpha and HNF-1 beta in various histological differentiations of hepatocellular carcinoma. J Pathol. 1998;184(3):272-8.
Wang, W., Hayashi, Y., Ninomiya, T., Ohta, K., Nakabayashi, H., Tamaoki, T., & Itoh, H. (1998). Expression of HNF-1 alpha and HNF-1 beta in various histological differentiations of hepatocellular carcinoma. The Journal of Pathology, 184(3), 272-8.
Wang W, et al. Expression of HNF-1 Alpha and HNF-1 Beta in Various Histological Differentiations of Hepatocellular Carcinoma. J Pathol. 1998;184(3):272-8. PubMed PMID: 9614379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of HNF-1 alpha and HNF-1 beta in various histological differentiations of hepatocellular carcinoma. AU - Wang,W, AU - Hayashi,Y, AU - Ninomiya,T, AU - Ohta,K, AU - Nakabayashi,H, AU - Tamaoki,T, AU - Itoh,H, PY - 1998/6/6/pubmed PY - 2000/6/20/medline PY - 1998/6/6/entrez SP - 272 EP - 8 JF - The Journal of pathology JO - J Pathol VL - 184 IS - 3 N2 - Hepatic nuclear factor 1 (HNF-1) regulates genes in a hepatocyte-specific manner. It has been previously reported that the ratio of HNF-1 alpha and HNF-1 beta mRNA is related to histological differentiation hepatocellular carcinoma (HCC). In this study, the expression levels of the HNF-1 alpha and HNF-1 beta proteins were analysed relatively and quantitatively in various histologically differentiated HCC and surrounding non-cancerous tissues, and HNF-1 alpha binding activity for the AT element of the B domain of the human alpha-fetoprotein enhancer was examined. Western blot analysis demonstrated that HNF-1 alpha protein was expressed at a higher level in well-differentiated HCC tissues than in the surrounding non-HCC tissues; on the other hand, the HNF-1 alpha protein was expressed at lower levels in moderately and poorly differentiated HCCs than in the surrounding non-HCC tissues. The levels of HNF-1 beta expression in well-differentiated and poorly differentiated HCCs were similar to and higher than those found in the respective surrounding non-cancerous portions. In binding assays, HNF-1 binding activity was high in well-differentiated HCC and lower in moderately and poorly differentiated HCCs. Most well-differentiated HCC cases showed immunohistochemical expression of HNF-1 alpha. These findings show that poor histological differentiation of HCC correlates with decreases in the level and activity of HNF-1 alpha proteins. SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/9614379/Expression_of_HNF_1_alpha_and_HNF_1_beta_in_various_histological_differentiations_of_hepatocellular_carcinoma_ L2 - https://doi.org/10.1002/(SICI)1096-9896(199803)184:3<272::AID-PATH4>3.0.CO;2-K DB - PRIME DP - Unbound Medicine ER -