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Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching.
Genes Dev. 1998 Jun 01; 12(11):1726-37.GD

Abstract

Switching of Saccharomyces mating type by replacement of sequences at the MAT locus involves a choice between two donors, HML and HMR. MATalpha cells inhibit recombination along the entire left arm of chromosome III, including HML, whereas MATa cells activate this same region. MATa-dependent activation of HML depends on a small, cis-acting DNA sequence designated the recombination enhancer (RE), located 17 kb centromere-proximal to HML. A comparison of RE sequences interchangeable between Saccharomyces cerevisiae and Saccharomyces carlsbergensis defines a minimum RE of 244 bp. RE activity is repressed in MATalpha cells by binding of the Matalpha2-Mcm1 corepressor to a site within the RE. Mutation of the two Matalpha2 binding sites removes most, but not all, of this repression, and RE chromatin structure in MATalpha cells becomes indistinguishable from that seen in MATa. Surprisingly, a 2-bp mutation in the Mcm1 binding site completely abolishes RE activity in MATa cells; moreover, RE chromatin structure in the MATa mutant becomes very similar to that seen in MATalpha cells with a normal RE, displaying highly ordered nucleosomes despite the absence of Matalpha2. Further, a mutation that alters the ability of Mcm1 to act with Matalpha2 in repressing a-specific genes also alters donor preference in either mating type. Thus, Mcm1 is critically responsible for the activation as well as the Matalpha2-Mcm1-mediated repression of RE activity.

Authors+Show Affiliations

Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110 USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

9620858

Citation

Wu, C, et al. "Mcm1 Regulates Donor Preference Controlled By the Recombination Enhancer in Saccharomyces Mating-type Switching." Genes & Development, vol. 12, no. 11, 1998, pp. 1726-37.
Wu C, Weiss K, Yang C, et al. Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching. Genes Dev. 1998;12(11):1726-37.
Wu, C., Weiss, K., Yang, C., Harris, M. A., Tye, B. K., Newlon, C. S., Simpson, R. T., & Haber, J. E. (1998). Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching. Genes & Development, 12(11), 1726-37.
Wu C, et al. Mcm1 Regulates Donor Preference Controlled By the Recombination Enhancer in Saccharomyces Mating-type Switching. Genes Dev. 1998 Jun 1;12(11):1726-37. PubMed PMID: 9620858.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching. AU - Wu,C, AU - Weiss,K, AU - Yang,C, AU - Harris,M A, AU - Tye,B K, AU - Newlon,C S, AU - Simpson,R T, AU - Haber,J E, PY - 1998/6/17/pubmed PY - 1998/6/17/medline PY - 1998/6/17/entrez SP - 1726 EP - 37 JF - Genes & development JO - Genes Dev VL - 12 IS - 11 N2 - Switching of Saccharomyces mating type by replacement of sequences at the MAT locus involves a choice between two donors, HML and HMR. MATalpha cells inhibit recombination along the entire left arm of chromosome III, including HML, whereas MATa cells activate this same region. MATa-dependent activation of HML depends on a small, cis-acting DNA sequence designated the recombination enhancer (RE), located 17 kb centromere-proximal to HML. A comparison of RE sequences interchangeable between Saccharomyces cerevisiae and Saccharomyces carlsbergensis defines a minimum RE of 244 bp. RE activity is repressed in MATalpha cells by binding of the Matalpha2-Mcm1 corepressor to a site within the RE. Mutation of the two Matalpha2 binding sites removes most, but not all, of this repression, and RE chromatin structure in MATalpha cells becomes indistinguishable from that seen in MATa. Surprisingly, a 2-bp mutation in the Mcm1 binding site completely abolishes RE activity in MATa cells; moreover, RE chromatin structure in the MATa mutant becomes very similar to that seen in MATalpha cells with a normal RE, displaying highly ordered nucleosomes despite the absence of Matalpha2. Further, a mutation that alters the ability of Mcm1 to act with Matalpha2 in repressing a-specific genes also alters donor preference in either mating type. Thus, Mcm1 is critically responsible for the activation as well as the Matalpha2-Mcm1-mediated repression of RE activity. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/9620858/Mcm1_regulates_donor_preference_controlled_by_the_recombination_enhancer_in_Saccharomyces_mating_type_switching_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=9620858 DB - PRIME DP - Unbound Medicine ER -