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GABAA and GABAC receptors on mammalian rod bipolar cells.
J Comp Neurol 1998; 396(3):351-65JC

Abstract

Rod bipolar (RB) cells of mammalian retinae receive synapses from different gamma-aminobutyric acid (GABAergic) amacrine cells in the inner plexiform layer (IPL). We addressed the question whether RB cells of the rabbit and of the rat retina express different types of GABA receptors at these synapses. RB cells were immunolabeled in vertical sections of rat retinae with an antibody against protein kinase C (PKC). The sections were double-labeled for the alpha 1, alpha 2, alpha 3, or gamma 2 subunits of the GABAA receptor. Punctate immunofluorescence, which represents synaptic localization, was found for all four subunits. Many of the alpha 1-, alpha 3-, or gamma 2-immunoreactive puncta coincided with the axon terminals of the PKC-immunolabeled RB cells. Sections and wholemounts of rabbit retinae were also double labeled for PKC and the rho subunits of the GABAC receptor. Rabbit RB cells were decorated by many rho-immunoreactive puncta, which were shown by electron microscopy to represent synaptic localization. Previous work from our laboratory has shown that the alpha 1, alpha 2, alpha 3, and rho subunits are not found within the same synapse but are expressed at different synaptic sites. Taken together, these results suggest that RB cells of mammalian retinae express at least three different types of GABA receptors at synaptic sites in the IPL: GABAC receptors, GABAA receptors containing the alpha 1 subunit, and GABAA receptors containing the alpha 3 subunit.

Authors+Show Affiliations

Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

9624589

Citation

Fletcher, E L., et al. "GABAA and GABAC Receptors On Mammalian Rod Bipolar Cells." The Journal of Comparative Neurology, vol. 396, no. 3, 1998, pp. 351-65.
Fletcher EL, Koulen P, Wässle H. GABAA and GABAC receptors on mammalian rod bipolar cells. J Comp Neurol. 1998;396(3):351-65.
Fletcher, E. L., Koulen, P., & Wässle, H. (1998). GABAA and GABAC receptors on mammalian rod bipolar cells. The Journal of Comparative Neurology, 396(3), pp. 351-65.
Fletcher EL, Koulen P, Wässle H. GABAA and GABAC Receptors On Mammalian Rod Bipolar Cells. J Comp Neurol. 1998 Jul 6;396(3):351-65. PubMed PMID: 9624589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GABAA and GABAC receptors on mammalian rod bipolar cells. AU - Fletcher,E L, AU - Koulen,P, AU - Wässle,H, PY - 1998/6/13/pubmed PY - 2000/6/20/medline PY - 1998/6/13/entrez SP - 351 EP - 65 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 396 IS - 3 N2 - Rod bipolar (RB) cells of mammalian retinae receive synapses from different gamma-aminobutyric acid (GABAergic) amacrine cells in the inner plexiform layer (IPL). We addressed the question whether RB cells of the rabbit and of the rat retina express different types of GABA receptors at these synapses. RB cells were immunolabeled in vertical sections of rat retinae with an antibody against protein kinase C (PKC). The sections were double-labeled for the alpha 1, alpha 2, alpha 3, or gamma 2 subunits of the GABAA receptor. Punctate immunofluorescence, which represents synaptic localization, was found for all four subunits. Many of the alpha 1-, alpha 3-, or gamma 2-immunoreactive puncta coincided with the axon terminals of the PKC-immunolabeled RB cells. Sections and wholemounts of rabbit retinae were also double labeled for PKC and the rho subunits of the GABAC receptor. Rabbit RB cells were decorated by many rho-immunoreactive puncta, which were shown by electron microscopy to represent synaptic localization. Previous work from our laboratory has shown that the alpha 1, alpha 2, alpha 3, and rho subunits are not found within the same synapse but are expressed at different synaptic sites. Taken together, these results suggest that RB cells of mammalian retinae express at least three different types of GABA receptors at synaptic sites in the IPL: GABAC receptors, GABAA receptors containing the alpha 1 subunit, and GABAA receptors containing the alpha 3 subunit. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/9624589/GABAA_and_GABAC_receptors_on_mammalian_rod_bipolar_cells_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1998&volume=396&issue=3&spage=351 DB - PRIME DP - Unbound Medicine ER -